614 Modulation of NK cell activation by calcium from alginate dressings in vitro

Adib, Yara; Boy, Maxime; Serror, Kévin; Dulphy, Nicolas; Courtils, C des; Duciel, Laura; Mimoun, M.; Samardzic, M.; Bagot, M.; Michel, Lise

doi:10.1016/j.jid.2022.09.631

Cited by 1 publication

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“…The amount of Ca 2+ released by ALG has been shown to induce calcium signalling and stimulate endothelial cell activation (Duciel et al., in preparation). Thus, the difference in Ca 2+ released between ALG and BIA could explain the differences observed in a previous study on NK cell activation 24 and in this study on fibroblast activation. Moreover, some dressings containing CMC have been reported to reduce fibroblast viability and proliferation and CMC inhibited NK cell cytolytic activity 16,24 …”

Section: Discussioncontrasting

confidence: 44%

“…In a previous study, ALG‐CM was shown to enhance NK cell activities, notably pro‐inflammatory cytokine production, which suggested that Algosteril stimulated NK cell‐mediated defence during the inflammatory phase 24 . However, an inflammatory environment affects fibroblast activities necessary for granulation tissue formation and must be resolved for healing to proceed 3 .…”

Section: Discussionmentioning

confidence: 99%

“…These differences in composition will impact the amount of Ca 2+ ions released by alginate dressings into the wound 36 . Previous studies have shown that ALG releases a different amount of Ca 2+ than BIA 24 . Ca 2+ , via calcium signalling, is a powerful cell activator 37 .…”

Section: Discussionmentioning

confidence: 99%

“…NK cells were purified from isolated human peripheral blood mononuclear cells (PBMCs) as previously described. 24 NK cells first incubated with IL-2/IL-15 in complete CT RPMI1640 during 24 h, were then stimulated by the presence of LPS (1 μg/mL) in either CT or ALG CM for a further 18 h period.…”

Section: Effect Of Nk Cell Supernatant On Fibroblast Migrationmentioning

confidence: 99%

“…ALG-CM was previously shown to stimulate activated NK cells, 24 and these cells may inhibit fibroblast migration. 4,5,7 Therefore, the effect of the supernatant of NK cells incubated or not with ALG-CM was analysed on fibroblast migration.…”

Section: Migration Of Fibroblasts In the Presence Of Nk Supernatantsmentioning

confidence: 99%

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In vitro effects of wound‐dressings on key wound healing properties of dermal fibroblasts

Peltier,

Adib,

Nicosia

et al. 2024

Experimental Dermatology

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Healing of complex wounds requires dressings that must, at least, not hinder and should ideally promote the activity of key healing cells, in particular fibroblasts. This in vitro study assessed the effects of three wound‐dressings (a pure Ca2+ alginate: Algostéril®, a Ca2+ alginate + carboxymethylcellulose: Biatain alginate® and a polyacrylate impregnated with lipido‐colloid matrix: UrgoClean®) on dermal fibroblast activity. The results showed the pure calcium alginate to be non‐cytotoxic, whereas the other wound‐dressings showed moderate to strong cytotoxicity. The two alginates stimulated fibroblast migration and proliferation, whereas the polyacrylate altered migration and had no effect on proliferation. The pure Ca2+ alginate significantly increased the TGF‐β‐induced fibroblast activation, which is essential to healing. This activation was confirmed by a significant increase in Vascular endothelial growth factor (VEGF) secretion and a higher collagen production. The other dressings reduced these fibroblast activities. The pure Ca2+ alginate was also able to counteract the inhibitory effect of NK cell supernatants on fibroblast migration. These in vitro results demonstrate that tested wound‐dressings are not equivalent for fibroblast activation. Only Algostéril was found to promote all the fibroblast activities tested, which could contribute to its healing efficacy demonstrated in the clinic.

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Section: Discussioncontrasting

confidence: 44%

Section: Discussionmentioning

confidence: 99%