60Co-γ Radiation Alters Developmental Stages of Zeugodacus cucurbitae (Diptera: Tephritidae) Through Apoptosis Pathways Gene Expression

Abstract

Radiation is considered as a promising insect pest control strategy for minimizing postharvest yield losses. Among various techniques, irradiation is a method of choice as it induces lethal biochemical or molecular changes that cause a downstream cascade of abrupt physiological abnormalities at the cellular level. In this study, we evaluated the effect of 60 Co-γ radiation on various developmental stages of Zeugodacus cucurbitae Coquillett and subsequent carry-over effects on the progeny. For this purpose, we treated eggs with 30- and 50-Gy radiation doses of 60 Co-γ. We found that radiation significantly affected cellular antioxidants, insect morphology, and gene expression profiles. Our results indicate that in response to various doses of irradiation reactive oxygen species, catalase, peroxidase, and superoxide dismutase activities were increased along with a significant increase in the malondialdehyde (MDA) content. We observed higher mortality rates during the pupal stage of the insects that hatched from irradiated eggs (50 Gy). Furthermore, the life span of the adults was reduced in response to 50 Gy radiation. The negative effects carried over to the next generation were marked by significantly lower fecundity in the F 1 generation of the irradiation groups as compared to control. The radiation induced morphological abnormalities at the pupal, as well as the adult, stages. Furthermore, variations in the gene expression following irradiation are discussed. Taken together, our results signify the utility of 60 Co-γ radiation for fruit fly postharvest management.

Comparative transcriptomics of the irradiated melon fly (Zeugodacus cucurbitae) reveal key developmental genes

“…25 h old eggs were exposed to 60 Co radiation at 50 Gy under free oxygen, at 1.0 Gy/min following previously published studies ( Cai et al, 2018 ; Ahmad et al, 2021a ). Experimental replicates were composed of three Petri dishes each containing 100 eggs (diameter of 100 mm; height 15 mm), and three replicates per treatment (irradiated versus control) were prepared.…”

Neuroprotective effect of Ginsenoside Re against neurotoxin‑induced Parkinson's disease models via induction of Nrf2

“…CA1410; Beijing Solarbio Science & Technology Co., Ltd.) for 30 min at 37°C. ROS was measured with an Infinite ® 200 Pro microplate reader (Tecan Group, Ltd.) using excitation at 488 nm and emission at 530 nm ( 20 ).…”

Reactive Oxygen Species in the Adverse Outcome Pathway Framework: Toward Creation of Harmonized Consensus Key Events

“…Comprehensive mechanisms of ROS-dependent and independent DNA damage induced by ionizing radiation and solar radiation have been proposed ( Cadet and Wagner, 2013 ; Helm and Rudel, 2020 ). Radiation can also increase antioxidants such as SOD, CAT, and peroxidase (POD) ( Seen and Tong, 2018 ; Ahmad et al, 2021 ). Radiation-induced DNA damage and ROS can also lead to cell death and autophagy and are utilized for this reason in cancer treatment ( Ma et al, 2021 ; Yamamoto et al, 2021 ).…”