6‐Triphenylphosphinehexanoic Acid Conjugated Near‐Infrared Persistent Luminescence Nanoprobe for Autofluorescence‐Free Targeted Imaging of Mitochondria in Cancer Cells

Abstract

Mitochondria, as the energy provider for cells, play a key role in cell physiological processes. Mitochondrion‐targeted imaging is of great significance for understanding the physiological functions and pathology involved in various diseases. Herein, we report the fabrication of 6‐triphenylphosphinehexanoic acid (TPP) conjugated near‐infrared persistent luminescence nanoparticles (PLNP) (PLNP‐TPP) for mitochondria‐targeted imaging in cancer cells. The PLNP was prepared by doping Cr(III) into gallogermanate through a hydrothermal method. The as‐prepared PLNP showed small size, monodispersity, excellent near‐infrared (NIR) luminescence and visible light renewable NIR luminescence. The grafted TPP facilitated the transportation of PLNP‐TPP across the mitochondrial membrane and enabled staining of the mitochondrial region in live cancer cells. This work provides a promising strategy for fabricating PLNP nanoprobes to realize mitochondrial‐targeting bioimaging.

68Ga-Labeled Magnetic-NIR Persistent Luminescent Hybrid Mesoporous Nanoparticles for Multimodal Imaging-Guided Chemotherapy and Photodynamic Therapy

“…Near-infrared persistent luminescence nanoparticles (NIR-PLNPs) are unique luminescent materials that can emit NIR-PL within the biologically optical window for an appreciable time after the turnoff of excitation light. − Owing to such superior optical properties, NIR-PLNPs enable complete avoidance of tissue autofluorescence and superior signal-to-noise ratios (SNR) in optical detection, making these optical probes highly suitable for biosensing, − cell tracking, − and in vivo deep-tissue imaging. − However, like other conventional optical probes, − NIR-PLNPs can provide high planar resolution and hypersensitive cellular-level imaging, but they lack spatial resolution and abilities to obtain detailed anatomy and physiology information in vivo . − To address these shortcomings, integrating NIR-PLNPs with various imaging techniques, such as computed tomography (CT), , magnetic resonance (MR) imaging, ,,− and single-photon emission computed tomography (SPECT), , has captured special attention of scientists and clinicians over the recent decade.…”