6-Methylsulfinylhexyl Isothiocyanate, an Antioxidant Derived from Wasabia japonica MATUM, Ameliorates Diabetic Nephropathy in Type 2 Diabetic Mice

Abstract

Many studies have examined the protective effects of antioxidative agents against diabetic nephropathy, using various models. 6-Methylsulfinylhexyl isothiocyanate (6-MSITC) isolated from wasabi (Wasabiajaponica MATUM) induces glutathione S-transferase in vitro, thus 6-MSITC may act as an antioxidant in vivo. The aim of this study was to examine whether wasabi powder (WP) and 6-MSITC suppress oxidative stress in vivo and inhibit the impairment of renal function and diabetic nephropathy, using type 2 diabetic mice. KK-Ay type 2 diabetic mice were assigned to three groups (n = 10 each); control mice were fed normal chow (CRF-1) and two experimental groups were fed CRF-1 containing 0.5% WP or 0.03% 6-MSITC for 4 wk. Urine volume, urinary albumin excretion, and creatinine clearance were significantly lower in the 6-MSITC group than in the control group. There was statistically no difference in TBARS or other biomarkers of oxidative stress among the three groups. However, urinary 8-hydroxy-2'-deoxyguanosine (8-0HdG), one of the markers of oxidative stress tended to be lower in the 6-MSITC group than in the control group. In conclusion, the present results show that a sufficient supply of dietary 6-MSITC may prevent or delay renal dysfunction in diabetes by protecting against oxidative stress, and that dietary 6-MSITC may have beneficial effects on diabetic complications in type 2 diabetic mice.

Antioxidant and pro‐oxidant capacities of ITCs

“…6-Methylsulfinylhexyl ITC (hesperin), an ITC isolated from wasabi (Wasabia japonica), protected from oxidative stress and ameliorated renal dysfunction in type 2 diabetic mice. In this study, KK-Ay type 2 diabetic mice were fed CRF-1 containing 0.03% hesperin for 4 weeks: there was statistically no difference in TBARS, however, urinary 8hydroxy-2 0 -deoxyguanosine (8-OHdG), one of the markers of oxidative stress tended to be lower in the hesperin group than in the control group [Fukuchi et al, 2004]. Unlike N-acetylcysteine (NAC) that produced no improvement, phenethyl ITC 0.5% added to drinking water for 4 weeks effectively lowered oxidative-stressmediated testicular toxicity, induced by coadministration of acrylamide 0.02%, in male Sprague-Dawley rats .…”

Anti-obesity effects of hot water extract from Wasabi (Wasabia japonica Matsum.) leaves in mice fed high-fat diets

“…In Japan, wasabi is used as a spice or as pickles due to its strong pungent constituents, including isothiocyanates such as allyisothiocyanate. Previous studies have reported on antimicrobial [9], antioxidative [10] and anti-carcinogenic activities [11] of isothiocyanate. However, few studies have examined the functional properties of other components in wasabi, particularly in its summer leaves, which are considered late-growth and have little pungency [12].…”

A Review of Propagation Techniques and Isothiocyanates Content in Wasabi (Wasabia japonica Matsum.)

“…According to other studies, Wasabi leafstalk extract stimulates bones calcification and it might be used in osteoporosis prevention (Suzuki et al, 1997;Suzuki and Masayoshi, 2004;Yamaguchi, 2016). 6-methylsulfinylhexyl isothiocyanate isolated from Wasabi has positive antioxidant effects in diabetes renal dysfunction (Fukuchi et al, 2004). The research also shows a possible use of transgenesis of a wasabi gene; in potatoes, the wasabi gene has a positive role in partial resistance to Botrytis cinerea (gray mold) (Khan et al, 2006).…”