6‐Methyl‐Δ^8,9‐ergolen‐8‐carbonsäure, ein neues Ergolinderivat aus Kulturen eines Stammes von Claviceps paspali STEVENSet HALL. 60. Mitteilung über Mutterkornalkaloide

New aspects of submerged fermentation of Claviceps paspali

“…Spores from a slant culture were washed off into 60 ml (106/ml) SP medium in edenmeyer flasks (300 ml) and incubated for 4-6 days in the dark at 23___ I°C on a rotary shaker (4.2 Hz, eccentricity, 5.5 cm). The resulting vegetative inoculum was used to inoculate (6 ml) production medium D (Kobel et al 1964, containing per g/1 tap H20): sorbitol, 50; succinic acid, 36; KH2PO~, 2; MgSOa-7H20, 0.3; FeSO4.7H20, 0.001 ; ZnSO4.7H20, 0.01 ; pH 5.4 adjusted with 10% NHaOH. Analytical methods.…”

Degenerationsprobleme bei Produktionsstämmen von Claviceps

“…Mikrobiologische Laboratorien des pharmazeutischen Departements der Sandoz AG, Basel ö-Methyl-l8 "-ergolen-8-karbonsäure produzierende Stämme von Claviceps paspali [3] Aus diesen Befunden wird geschlossen, dass es sich hier um die Mutation eines labilen, pleiotropen Gens handelt, welches fol gende Merkmale beeinflusst: Sklerotinisierung der Zellen. Pigmentsynthese, Synthese des Ergolingcrüstes, Virulenz.…”

Analysis of paspalic acid, lysergic acid, and iso‐lysergic acid by capillary zone electrophoresis with UV‐ and quadrupole time‐of‐flight mass spectrometric detection

“…One of the most prominent representatives within the group of ergot alkaloids is lysergic acid, a compound produced in substantial amounts for use as a pharmaceutical. One common approach for the production of this substance is the submerged fermentation of Claviceps paspali leading to paspalic acid, which can subsequently be transformed to lysergic acid using strong bases such as KOH or NaOH . As this isomerization process will never be complete, careful cleanup of the product is mandatory.…”