6-Gingerol-rich fraction prevents disruption of histomorphometry and marker enzymes of testicular function in carbendazim-treated rats

Salihu, Mariama; Ajayi, Babajide O.; Adedara, Isaac A.; Farombi, Ebenezer O.

doi:10.1111/and.12782

Cited by 23 publications

(13 citation statements)

References 39 publications

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“…63 Activities of free lysosomal enzymes have been shown to increase when testicular steroidogenesis is elevated. 64 However, a reduction in the ACP activity, as observed in DOX-treated rats, indicates decreased testicular steroidogenesis, and this may be correlated with reduced secretion of gonadotrophins. ALP is involved in the biosynthesis of nucleic acids, nuclear proteins, phospholipids, cleavage of phosphate esters, mobilizing carbohydrates and lipid metabolites for spermatozoa utilization.…”

Section: Discussionmentioning

confidence: 99%

Luteolin abates reproductive toxicity mediated by the oxido-inflammatory response in Doxorubicin-treated rats

Owumi

Ijadele

Arunsi

et al. 2020

Toxicology Research and Application

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The anti-neoplastic use of Doxorubicin (DOX) is hampered by several limitations, including reproductive toxicity. Luteolin (LUT)–a phytochemical-biological benefits include antioxidative and anti-inflammatory actions. Here we examined the protective effect of LUT against DOX-induced reproductive toxicity in an in vivo model—male albino Wistar rats—randomly assigned to five groups and treated as follows: Control (corn oil 2 mL/kg; per os), LUT (100 mg/kg; per os), DOX (2 mg/kg) by intraperitoneal injections, co-treated groups received LUT (50 and 100 mg/kg) with DOX. Treatment with DOX alone, significantly (p > 0.05), reduced biomarkers of testicular function, reproductive hormone levels, testicular and epididymal antioxidant, and anti-inflammatory cytokine. DOX increased (p > 0.05) sperm morphological abnormalities, as well as reactive oxygen and nitrogen species, lipid peroxidation, xanthine oxidase, a pro-inflammatory cytokine, and apoptotic biomarkers. Furthermore, testicular and epididymal histological lesion complemented the observed biochemical changes in treated rats. LUT co-treatment resulted in a dosage-dependent improvement in rats’ survivability, antioxidants capacity, reduction in biomarkers of oxidative stress, pro-inflammatory cytokines, and apoptosis in rat’s testis and epididymis. Also, LUT treatment resulted in improved histological features in the testis and epididymis, relative to DOX alone treated rats. LUT co-treatment abated DOX-mediated reproductive organ injuries associated with pro-oxidative, inflammatory, and apoptotic mechanisms. LUT supplementation may serve as a phyto-protective agent in alleviating male reproductive organ toxic injuries associated with Doxorubicin therapy.

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Section: Discussionmentioning

confidence: 99%

Luteolin abates reproductive toxicity mediated by the oxido-inflammatory response in Doxorubicin-treated rats

Owumi

Ijadele

Arunsi

et al. 2020

Toxicology Research and Application

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“…These enzymes are acknowledged indices of testicular function commonly assessed in reproductive toxicology. [ 40 ] Testicular ALP hydrolyses 6‐phosphoglucose, an intermediate in the glycolytic pathway, to free glucose which is essential for the proliferation and differentiation of spermatogenic cells. Testicular ACP activity is largely linked to functionality and survival of spermatogonial stem cell.…”

Section: Discussionmentioning

confidence: 99%

“…The activity of glucose‐6‐phosphate dehydrogenase (G6PD) was analyzed as previously described. [ 40 ] Lactate dehydrogenase‐X (LDH‐X) was analyzed using an established procedure. [ 41 ] Acid phosphatase (ACP) activity was assayed in agreement with the established method [ 27 ] whereas alkaline phosphatase (ALP) activity was assayed as previously described.…”

Section: Methodsmentioning

confidence: 99%

Abatement of the dysfunctional hypothalamic–pituitary–gonadal axis due to ciprofloxacin administration by selenium in male rats

Adedara

Awogbindin

Mohammed

et al. 2021

J Biochem & Molecular Tox

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The present study examined the influence of selenium on ciprofloxacin‐mediated reproductive dysfunction in rats. The research design consisted of five groups of eight animals each. The rats were administered 135 mg/kg body weight of ciprofloxacin per se or simultaneously with selenium at 0.25 and 0.5 mg/kg for 15 uninterrupted days. Antioxidant and inflammatory indices were assayed using the testes, epididymis, and hypothalamus of the animals after sacrifice. Results revealed that ciprofloxacin treatment per se interfered with the reproductive axis as demonstrated by diminished serum hormonal levels, sperm quality, and enzymatic indices of testicular function, which were, however, abrogated following selenium co‐treatment. Besides this, administration of selenium attenuated the depletion of glutathione level, inhibition of catalase, superoxide dismutase, glutathione‐S‐transferase and glutathione peroxidase activities with a concomitant reduction in reactive oxygen and nitrogen species, and lipid peroxidation in ciprofloxacin‐treated in rats. Selenium treatment also mitigated ciprofloxacin‐mediated elevation in nitric oxide level and of myeloperoxidase activity as well as histological lesions in the animals. Overall, selenium attenuated impairment in the male reproductive axis due to ciprofloxacin treatment through abatement of inflammation and oxidative stress in rats.

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“…Briefly, the testicular activity of glucose-6-phosphate dehydrogenase (G6PD) was assayed using nicotinamide adenine dinucleotide phosphate and glucose-6-phosphate as substrates as earlier reported. 41,42 Testicular acid phosphatase (ACP) and alkaline phosphatase (ALP) activities were assayed following established practice based on the degradation of p-nitrophenyl-phosphate in acid and alkaline milieu, respectively. 43,44 Also, testicular lactate dehydrogenase-X (LDH-X) activity was assessed by a standard technique that depended on the inter-change of lactate and pyruvate.…”

Section: Methodsmentioning

confidence: 99%

Gallic acid enhances reproductive function by modulating oxido-inflammatory and apoptosis mediators in rats exposed to aflatoxin-B1

Owumi

Adedara

Akomolafe

et al. 2020

Exp Biol Med (Maywood)

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Aflatoxin B1 (AFB1) is reported to elicit adverse reproductive outcomes in animals. Gallic acid (GA) is known to exhibit antioxidant and inflammatory bioactivities. The impact of GA on AFB1-facilitated reproductive dysfunction is nonexistent in literature. This investigation elucidated GA protective effect on AFB1-induced reproductive toxicities in rats, exposed for 28 consecutive days to AFB1 (75 µg/kg), or co-treated with GA (20 or 40 mg/kg) body weight. AFB1 significantly (p < 0.05) reduced testicular function biomarkers, serum hormonal levels, and functional sperm characteristics in experimental animals. GA abated AFB1-induced increases (p < 0.05) in lipid peroxidation and reactive oxygen and nitrogen species, suppressed myeloperoxidase, interleukin-1β, nitric oxide, and tumor necrosis factor-α levels—inflammatory biomarkers—in testes, epididymis, and hypothalamus. Furthermore, GA improved antioxidant defenses and alleviated reduction in interleukin-10, caspase-3 activation, and histological variations in epididymis, testes, and hypothalamus of rats dosed with AFB1. Conclusively, GA enhanced reproductive function in AFB1-exposed rats by modulating inflammatory, oxidative stress, and apoptosis mediators. Impact statement Infertility resulting from reproductive deficiency can be stressful. Exposure to aflatoxin B1, a dietary mycotoxin prevalent in improperly stored grains, is reported to elicit reproductive insufficiencies and infertility. We, therefore, examined the likely beneficial effect of gallic acid (GA) a phytochemical, recognized to exhibit in vitro and in vivo pharmacological bioactivities against oxidative stress and related inflammatory damages in rats, since AFB1 toxicities are predicated on oxidative epoxide formation, in a bid to proffer new evidence to advance the field of nutriceutical application from plant-derived chemopreventive agents. Our findings will advance the field of chemoprevention by presenting data absent in the literature on GA. Our results demonstrate further evidence for GA conferred protection against AFB1-mediated histological lesions in testes, epididymis, and hypothalamus of treated rats; suppresses oxidative damages, relieved inflammatory and apoptotic responses, restored sperm functional characteristics, and hormonal levels relevant for reproductive integrity and function.

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6-Gingerol-rich fraction prevents disruption of histomorphometry and marker enzymes of testicular function in carbendazim-treated rats

Cited by 23 publications

References 39 publications

Luteolin abates reproductive toxicity mediated by the oxido-inflammatory response in Doxorubicin-treated rats

Luteolin abates reproductive toxicity mediated by the oxido-inflammatory response in Doxorubicin-treated rats

Abatement of the dysfunctional hypothalamic–pituitary–gonadal axis due to ciprofloxacin administration by selenium in male rats

Gallic acid enhances reproductive function by modulating oxido-inflammatory and apoptosis mediators in rats exposed to aflatoxin-B1

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