6-Gingerol reduces Pseudomonas aeruginosa biofilm formation and virulence via quorum sensing inhibition

Kim, Han Shin; Lee, Sang Hoon; Byun, Youngjoo; Park, Hee Deung

doi:10.1038/srep08656

Cited by 233 publications

(192 citation statements)

References 52 publications

(57 reference statements)

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“…A pungent oil of fresh ginger (6-gingerol) adhered to QS receptors in P. aeruginosa and paused biofilm maturation. Transcriptomic analysis confirmed that 6-gingerol inhibited QS-induced gene expression and the production of virulence factors (81).…”

Section: Quorum-sensing Inhibitors: Stop Talking Help Killingmentioning

confidence: 83%

Control of Biofilm Formation: Antibiotics and Beyond

Algburi

Comito

Kashtanov

et al. 2017

Appl Environ Microbiol

193

146

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Biofilm-associated bacteria are less sensitive to antibiotics than freeliving (planktonic) cells. Furthermore, with variations in the concentration of antibiotics throughout a biofilm, microbial cells are often exposed to levels below inhibitory concentrations and may develop resistance. This, as well as the irresponsible use of antibiotics, leads to the selection of pathogens that are difficult to eradicate. The Centers for Disease Control and Prevention use the terms "antibiotic" and "antimicrobial agent" interchangeably. However, a clear distinction between these two terms is required for the purpose of this assessment. Therefore, we define "antibiotics" as pharmaceutically formulated and medically administered substances and "antimicrobials" as a broad category of substances which are not regulated as drugs. This comprehensive minireview evaluates the effect of natural antimicrobials on pathogens in biofilms when used instead of, or in combination with, commonly prescribed antibiotics.

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Section: Quorum-sensing Inhibitors: Stop Talking Help Killingmentioning

confidence: 83%

Control of Biofilm Formation: Antibiotics and Beyond

Algburi

Comito

Kashtanov

et al. 2017

Appl Environ Microbiol

193

146

View full text Add to dashboard Cite

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“…The biofilm assay was conducted in 96-well polystyrene plates as previously described, with minor modifications61. P. aeruginosa PA01 was inoculated into LB medium at an initial OD 600 of 0.05 and cultured with cyclo(Trp-Ser), DMSO or AZM at 37 °C for 24 h. The broth was discarded by washing with PBS three times.…”

Section: Methodsmentioning

confidence: 99%

A diketopiperazine factor from Rheinheimera aquimaris QSI02 exhibits anti-quorum sensing activity

Sun

Dai

Sun

et al. 2016

Sci Rep

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An ethyl acetate (EtOAc) extract isolated from the marine bacterium, Rheinheimera aquimaris QSI02, was found to exhibit anti-quorum sensing (anti-QS) activity. A subsequent bioassay-guided isolation protocol led to the detection of an active diketopiperazine factor, cyclo(Trp-Ser). Biosensor assay data showed that the minimum inhibitory concentration (MIC) of cyclo(Trp-Ser) ranged from 3.2 mg/ml to 6.4 mg/m for several microorganisms, including Escherichia coli, Chromobacterium violaceum CV026, Pseudomonas aeruginosa PA01, Staphylococcus aureus, and Candida albicans. Additionally, sub-MICs of cyclo(Trp-Ser) decreased the QS-regulated violacein production in C. violaceum CV026 by 67%. Furthermore, cyclo(Trp-Ser) can decrease QS-regulated pyocyanin production, elastase activity and biofilm formation in P. aeruginosa PA01 by 65%, 40% and 59.9%, respectively. Molecular docking results revealed that cyclo(Trp-Ser) binds to CviR receptor more rigidly than C6HSL with lower docking energy −8.68 kcal/mol, while with higher binding energy of −8.40 kcal/mol than 3-oxo-C12HSL in LasR receptor. Molecular dynamics simulation suggested that cyclo(Trp-Ser) is more easy to bind to CviR receptor than natural signaling molecule, but opposite in LasR receptor. These results suggest that cyclo(Trp-Ser) can be used as a potential inhibitor to control QS systems of C. violaceum and P. aeruginosa and provide increased the understanding of molecular mechanism that influences QS-regulated behaviors.

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“…Emergence of multi-drug resistant bacteria has driven studies for disabling virulence preferentially through quorum sensing (QS) inhibition tactics instead of bactericidal and bacteriostatic strategies (Singh et al, 2009a; Defoirdt et al, 2013; Kim et al, 2015; Lidor et al, 2015; Srivastava et al, 2015; Wang et al, 2015; Yang et al, 2015). P. aeruginosa , a Gram-negative bacterium causes cystic fibrosis in immunocompromised patients, burn units of hospitals, and in implanted medical devices including intubation tubes and stents (Singh et al, 2012; Singh B. R. et al, 2015; Kalferstova et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Scaffold of Selenium Nanovectors and Honey Phytochemicals for Inhibition of Pseudomonas aeruginosa Quorum Sensing and Biofilm Formation

Prateeksha

Singh

Shoeb

et al. 2017

Front. Cell. Infect. Microbiol.

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Honey is an excellent source of polyphenolic compounds that are effective in attenuating quorum sensing (QS), a chemical process of cell-to-cell communication system used by the opportunistic pathogen Pseudomonas aeruginosa to regulate virulence and biofilm formation. However, lower water solubility and inadequate bioavailability remains major concerns of these therapeutic polyphenols. Its therapeutic index can be improved by using nano-carrier systems to target QS signaling potently. In the present study, we fabricated a unique drug delivery system comprising selenium nanoparticles (SeNPs; non-viral vectors) and polyphenols of honey (HP) for enhancement of anti-QS activity of HP against P. aeruginosa PAO1. The developed selenium nano-scaffold showed superior anti-QS activity, anti-biofilm efficacy, and anti-virulence potential in both in-vitro and in-vivo over its individual components, SeNPs and HP. LasR is inhibited by selenium nano-scaffold in-vitro. Using computational molecular docking studies, we have also demonstrated that the anti-virulence activity of selenium nano-scaffold is reliant on molecular binding that occurs between HP and the QS receptor LasR through hydrogen bonding and hydrophobic interactions. Our preliminary investigations with selenium-based nano-carriers hold significant promise to improve anti-virulence effectiveness of phytochemicals by enhancing effective intracellular delivery.

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6-Gingerol reduces Pseudomonas aeruginosa biofilm formation and virulence via quorum sensing inhibition

Cited by 233 publications

References 52 publications

Control of Biofilm Formation: Antibiotics and Beyond

Control of Biofilm Formation: Antibiotics and Beyond

A diketopiperazine factor from Rheinheimera aquimaris QSI02 exhibits anti-quorum sensing activity

Scaffold of Selenium Nanovectors and Honey Phytochemicals for Inhibition of Pseudomonas aeruginosa Quorum Sensing and Biofilm Formation

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