6. Antimicrobial susceptibility of Salmonella enterica subsp. enterica serovar Choleraesuis strains from wild boar (Sus scrofa) in Italy

Donazzolo, C.; Turchetto, Sara; Ustulin, Martina; Citterio, Carlo Vittorio; Conedera, Gabriella; Vio, Denis; Deotto, Silvia; Giusto, Tiziana Di; Cocchi, Monia

doi:10.3920/978-90-8686-840-7_6

Cited by 2 publications

(6 citation statements)

References 22 publications

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“…Streptomycin is the aminoglycoside against which the highest prevalence of resistance has been found in this study, similarly to previous reports on Salmonella isolates from wild boar (Bonardi et al, 2019;Caleja et al, 2011a;Cilia et al, 2021;Donazzolo, 2017;Methner et al, 2010;Pinto et al, 2010;Zottola et al, 2013). The high proportion of isolates sharing streptomycin resistance is consistent with the frequency of genetic determinants found.…”

Section: Discussionsupporting

confidence: 92%

“…The frequent appearance of S. choleraesuis among the isolates of the present study is in line with the proportion of wild boars analysed with septicaemic processes, something uncommon in previous studies in this species. The high proportion of AMR observed in this serotype is in accordance with previous reports in this species (Donazzolo et al, 2017;Gil-Molino et al, 2020), although some studies did not find AMR in S. choleraesuis from European wild boars (Leekitcharoenphon et al, 2019). The frequency of appearance of AMR in the other subspecies from this study was lower than in S. enterica subsp.…”

Section: Discussionsupporting

confidence: 91%

“…AMR against β-lactams and quinolones are very rarely found in isolates of Salmonella spp. from wild boar (Bonardi et al, 2019;Donazzolo, 2017;Leekitcharoenphon et al, 2019); however, we described percentages of resistance to β-lactams close to 20% and of around 6%…”

Section: Discussionmentioning

confidence: 67%

“…The predominant resistant patterns detected in this study were SUL and TRS‐SUL, similarly to those previously reported for bacteria from wild boars, where a great variety of resistance patterns was described (Cilia et al., 2021 ; Zottola et al., 2013 ). In contrast, other studies described only a few AMR patterns, which might be attributed to differences in sampling methods and/or in environmental conditions of the wild boar population from which bacteria were isolated (Bonardi et al., 2019 ; Donazzolo, 2017 ; Methner et al., 2010 ).…”

Section: Discussionmentioning

confidence: 95%

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Dissemination of antimicrobial‐resistant isolates of Salmonella spp. in wild boars and its relationship with management practices

Molino

Gonçalves

Risco

et al. 2022

Transbounding Emerging Dis

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Antimicrobial resistance (AMR) is a global concern and controlling its spread is critical for the effectiveness of antibiotics. Members of the genus Salmonella are broadly distributed, and wild boar may play an important role in its circulation between periurban areas and the environment, due to its frequent interactions both with livestock or human garbage. As the population of these animals is rising due to management on certain hunting estates or the absence of natural predators, the aim of the present work is to identify the mechanisms of AMR present and/or expressed in Salmonella spp. from wild boar populations and to determine the possible role of managementrelated factors applied to different game estates located in central Spain. The detection of Salmonella spp. was carried out in 121 dead wild boar from 24 game estates, and antimicrobial resistance traits were determined by antibiotic susceptibility testing and screening for their genetic determinants. The effects of feeding supplementation, the proximity of livestock, the existence of a surrounding fence and the density of wild boar on the AMR of the isolates were evaluated. The predominant subspecies and serovar found were S. enterica subsp. enterica (n = 69) and S. choleraesuis (n = 33), respectively. The other subspecies found were S. enterica subsp. diarizonae, S. enterica subsp. salamae and S. enterica subsp. houtenae. AMR was common among isolates (75.2%) and 15.7% showed multi drug resistance (MDR). Resistance to sulphonamides was the most frequent (85.7%), as well as sul1 which was the AMR determinant most commonly found.Plasmids appeared in 38.8% of the isolates, with IncHI1 being the replicon detected with the highest prevalence. The AMR of the isolates increased when the animals were raised with feeding supplementation and enclosed by fences around the estates.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 67%

Section: Discussionmentioning

confidence: 95%

See 2 more Smart Citations

Dissemination of antimicrobial‐resistant isolates of Salmonella spp. in wild boars and its relationship with management practices

Molino

Gonçalves

Risco

et al. 2022

Transbounding Emerging Dis

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“…Studies from United States showed higher rates of resistance to tetracyclines (92.6%) (Huang, Lin, & Wu, 2009) while data from Japan revealed multidrug resistant isolates with resistance to fluoroquinolones and cephalosporines (Asai et al, 2010;Chang et al, 2005;Chiu et al, 2004). Other screening performed in wild boars from Germany revealed high resistance of S. Choleraesuis to sulphamethoxazole and streptomycin (Methner et al, 2010) whereas very high rates of resistance against streptomycin (73%), spiramicin, and tilmicosin (both at 100%) have been found in Italy (Donazzolo et al, 2017). To date, the present study is the only report that analyses the resistance genes and the plasmids replicons present in S. Choleraesuis isolates from wild boars.…”

Section: Discussionmentioning

confidence: 99%

Outbreaks of antimicrobial resistantSalmonellaCholeraesuis in wild boars piglets from central‐western Spain

Molino

Risco²,

Blanco³

et al. 2018

Transbound Emerg Dis

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Salmonella enterica serovar Choleraesuis is the aetiological agent of swine paratyphoid being a highly invasive zoonotic pathogen. Wild boar natural populations are experiencing a demographical expansion as well as some farms are breeding this species to release for hunting with management sometimes identical to that of domestic pigs, including supplementation, grouping, and antibiotic treatments. This situation increases the chance of contact between wild boars and livestock, and potentially induces stress, with different sanitary consequences. The present work aims to describe the clinical features of recent outbreaks caused by S. Choleraesuis in wild boar from central-western Spain, as well as the antimicrobial resistance and phylogenetic relationships of isolates involved. 28 strains of S. Choleraesuis were isolated from 28 different wild boars belonging to 10 different game states located in central western Spain and submitted to the Clinical Veterinary Hospital (CVH) of the University of Extremadura. Samples were taken from different organs and cultured according to the ISO 6579:2002 procedure. Suspicious colonies were identified by PCR and antimicrobial resistance was evaluated by disc diffusion susceptibility test and the presence of the main resistance genes as well as 18 plasmid replicons frequently found among the Enterobacteriaceae was verified by PCR. Pulsed field gel electrophoresis was applied to determine the genetic relationship between isolates. The outbreaks under study were characterized by high mortality (35%-84%) and a septicaemic presentation. S. Choleraesuis was isolated from all the wild boars analysed, and 26 of the 28 isolates presented resistance to at least one antibiotic. The predominant resistances found were against sulphonamide, streptomycin, tetracycline, and doxicicline and sul1, strA-strB, and tetA were the most prevalent resistance genes among isolates. 10 strains carried FIIA, FIB+H/1 or FIIA+H/1 plasmids. PFGE classified the isolates into four different profiles, grouped into two clusters. This results show that prevention against S. Choleraesuis must be considered in the sanitary programs of the wild boar breeders.

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6. Antimicrobial susceptibility of Salmonella enterica subsp. enterica serovar Choleraesuis strains from wild boar (Sus scrofa) in Italy

Cited by 2 publications

References 22 publications

Dissemination of antimicrobial‐resistant isolates of Salmonella spp. in wild boars and its relationship with management practices

Dissemination of antimicrobial‐resistant isolates of Salmonella spp. in wild boars and its relationship with management practices

Outbreaks of antimicrobial resistantSalmonellaCholeraesuis in wild boars piglets from central‐western Spain

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