6,7,4′-Trihydroxyflavanone Protects against Dextran Sulfate Sodium-Induced Colitis by Regulating the Activity of T Cells and Colon Cells In Vivo

Abstract

Colitis is a multifactorial disorder that mostly occurs in the gastrointestinal tract. Despite improvements in mucosal inflammation research, little is known regarding the small bioactive molecules that are beneficial for regulating T cells and colon cell activity. 6,7,4′-trihydroxyflavanone (THF) is a flavanone that possesses anti-osteoclastogenesis activity and exerts protective effects against methamphetamine-induced immunotoxicity. Whether THF mitigates intestinal inflammation by regulating T cells and colon cell activity remains unknown. In the present study, Jurkat and HT-29 cells were used for in vitro experiments, and dextran sulfate sodium (DSS)-induced colitis model in mice was used for in vivo experiment. We observed that THF did not have a negative effect on the viability of Jurkat and HT-29 cells. Quantitative PCR and Western blot analysis revealed that THF regulates the activity of Jurkat cells and HT-29 cells via the NFκB and MAPK pathways under stimulated conditions. In the DSS-induced colitis model, oral administration of THF attenuated the manifestations of DSS-induced colitis, including a reduction in body weight, shrinkage of the colon, and enhanced expression of pro-inflammatory cytokines in the colon and mesenteric lymph nodes. These data suggest that THF alleviates DSS-induced colitis by modulating the activity of T cells and colon cells in vivo.

A correlation study affecting survival in patients after radical colon cancer surgery: A retrospective study

“…[29] There are also studies showing no correlation between weight loss and prognosis of colon cancer, and most of these studies were also analyzed based on the presence or absence of weight loss, without statistical information such as the specific degree of reduction included in the analysis, which is consistent with the conclusion we reached in this study, so it is considered that the degree of weight loss is more significant in patients with advanced cancer, and early stage patients can also show weight loss as a manifestation, but to a lesser extent. [30] The presence or absence of weight loss is not a prognostic influence, while severe weight loss has a correlation with prognosis.…”

6,7,4′-Trihydroxyflavanone Mitigates Methamphetamine-Induced Neurotoxicity in SH-SY5y Cells via Nrf2/heme Oxyganase-1 and PI3K/Akt/mTOR Signaling Pathways

“…6,7,4′-trihydroxyflavanone (THF, C 15 H 12 O 5 ), a bioactive molecule of the flavanone family, is isolated from Dalbergia odorifera (Leguminosae) [ 23 ] and has been traditionally used for its anti-epigastric pain, anti-rheumatic, anti-ischemic, and anti-swelling properties [ 24 ]. Recent reports have shown that THF possesses several bioactivities against osteoclastogenesis in bone formation, exerting anti-inflammatory effects against dextran sodium sulfate (DSS)-induced immune bowel disease (IBD) and protective effects against hypoxia-induced neurotoxicity via the enhancement of the Nrf2/HO-1 signaling pathway [ 23 , 25 ]. In particular, the ameliorative effect of THF on METH-induced cytotoxicity has been studied in T cells, where pretreatment with THF significantly restored the METH-induced deactivation of T cells under stimulated conditions [ 26 ].…”