6.16 Identification of bovine B cell reactive and B cell specific monoclonal antibodies

Mukwedeya, D; Takamatsu, H.; Parkhouse, R. M. E.

doi:10.1016/0165-2427(93)90179-8

Cited by 11 publications

(6 citation statements)

References 10 publications

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“…B lymphocytes express a surface form of IgM and several non-immunoglobulin molecules (Morrison et al, 1988;Griebel et al, 1992;Mukwedeya et al, 1993). T lymphocytes that express the oB T cell receptor (TcR2) coexpress either BoCD4 (T helper lymphocytes) , or BoCD8 (T cytotoxic/suppressor lymphocytes) (Ellis et al, 1986).…”

Section: Introductionmentioning

confidence: 99%

Identification of γδT lymphocyte subsets that populate calf ileal mucosa after birth

Wyatt

Brackett

Perryman

et al. 1996

Veterinary Immunology and Immunopathology

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Ileal intraepithelial and lamina propria lymphocytes from newborn, 1.5-week-old, and 3-week-old calves were compared to determine to what extent the mucosa becomes populated after birth. Single and dual fluorescence flow cytometry were used with monoclonal antibodies to bovine (Bo) CD molecules to identify lymphocyte subpopulations. Few ileal mucosal lymphocytes were present in calves at birth. However, by 1.5 weeks of age, the villi were populated with large numbers of lymphocytes, and by 3 weeks of age, the numbers had increased further. These included a prominent subpopulation of gamma delta T cells. Several subsets of gamma delta T cells populated ileal mucosa after birth. The predominant subset coexpressed BoCD2, and a smaller subset coexpressed BoCD8. WC1+ gamma delta T cells comprised the smallest subset. All gamma delta T cell subsets coexpressed ACT2, a molecule expressed on activated WC1+ and WC1- gamma delta T cells from cattle.

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Section: Introductionmentioning

confidence: 99%

Identification of γδT lymphocyte subsets that populate calf ileal mucosa after birth

Wyatt

Brackett

Perryman

et al. 1996

Veterinary Immunology and Immunopathology

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“…Due to difficulties such as a failure to immunoprecipitate the Ag (principally due to the IgM isotype of the mAb), and the mAb not identifying any cells in frozen lymph node sections during a workshop study [2], the mAb had failed to be given any workshop cluster designation. By using both activated and resting lymphocytes, more sensitive immunohistology, and modified immunoprecipitation techniques, we have shown that the antigen is present both on B cells and activated T cells, and stains a specific zone of GCs in paraffin-embedded lymph node sections.…”

Section: Discussionmentioning

confidence: 99%

“…In a previous study, VPM30 was not found to stain ethanolacetic acid-fixed 8-µm frozen lymph node sections [2]. However, staining of 6-µm acetone-fixed frozen sections gave extremely characteristic staining patterns.…”

Section: Frozen Sectionsmentioning

confidence: 99%

“…Although reagents to define the basic lymphocyte subsets have been available for some time in ruminants, identification of molecules defining distinct stages of development, particularly in B cells, has proved difficult. In recent workshop reports, many monoclonal antibodies (mAb) were characterized, including the B cell markers sIg, MHC class II, CD19, and CD21 [1][2][3]. Although of benefit in routine phenotyping of leukocyte subsets, the expression of these markers on B cells in the periphery is constitutive, and is not of great use in dissecting developmental steps in immune response development.…”

Section: Introductionmentioning

confidence: 99%

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A novel cell surface proliferation-associated marker expressed on T cells and up-regulated on germinal center B cells

Campbell

Hopkins²,

Oliver

et al. 1998

Journal of Leukocyte Biology

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In this study we present data on a novel cell surface antigen recognized by monoclonal antibody (mAb) VPM30, originally thought to recognize only bovine and ovine sIg ؉ B cells from peripheral blood. Here we show that the antigen, molecular mass 28 kDa, is not only found in B cell follicles in frozen sections, but when used on paraffin sections VPM30 specifically stains B cells in the light zone of germinal centers but not in the mantle or dark zones. In addition we show that the antigen is also expressed by 90% of T cells after activation, with kinetics of antigen expression mirroring those of proliferation. By both size and distribution, the antigen appears to be novel, corresponding to no known cluster of differentiation, and will be of great use in the study of ruminant cellular immune responses.

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“…Monoclonal antibodies have been produced that define bovine T lymphocyte antigens CD3 , CD4 (Bensaid et al, 1991), CDS (Larsen et al, 1990), and gamma delta T cell receptor (TCR) (Davis et al, 1996a), as well as B cell markers such as CD21 (Mukwedeya et al, 1993;Sopp, 1996). Single color flow cytometry has been used to study mitogen induced changes in percentages of lymphocyte subsets (Hurley et al, 1994;Franklin et al, 1994) and surface expression of lymphocyte activation markers, such as Interleukin-2 receptora (IL2r) Prince et al, 1986) and major hisotcompatibility complex class II (MHC II) (Davis et al, 1987).…”

Section: Introductionmentioning

confidence: 99%

In vitro detection of antigen specific T lymphocyte subsets in cattle

Quade

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CHAPTER 1. GENERAL INTRODUCTION 1 Dissertation organization 1 Research summary 2 CHAPTER 2. LITERATURE REVIEW: IN VITRO DETECTION OF ANTIGEN SPECmC MEMORY T LYMPHOCYTES 4 Introduction: the importance of memory lymphocytes 4 What are memory lymphocytes? 5 Applications for detection of antigen specific memory lymphocytes 7 T lymphocyte subsets 10 Methods of detection in vitro of T cell activation 12 Summary 21 References 22 CHAPTER 3.

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6.16 Identification of bovine B cell reactive and B cell specific monoclonal antibodies

Cited by 11 publications

References 10 publications

Identification of γδT lymphocyte subsets that populate calf ileal mucosa after birth

Identification of γδT lymphocyte subsets that populate calf ileal mucosa after birth

A novel cell surface proliferation-associated marker expressed on T cells and up-regulated on germinal center B cells

In vitro detection of antigen specific T lymphocyte subsets in cattle

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