5α-reductase and 17β-hydroxysteroid dehydrogenase expression in epithelial cells from hyperplastic and malignant human prostate

Délos, Sylvie; Carsol, Jean-Louis; Fina, Frédéric; Raynaud, Jean‐Pierre; Martin, Pièrre‐Marie

doi:10.1002/(sici)1097-0215(19980316)75:6<840::aid-ijc5>3.0.co;2-v

Cited by 16 publications

(6 citation statements)

References 33 publications

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“…Both 5α‐reductase‐1 and 5α‐reductase‐2 isozymes are expressed in benign prostate hyperplasia and prostate cancer tissues (Smith et al, 1994). 5α‐Reductase isozymes are also expressed in prostate tumor cells, including LNCaP cells (Figure 3) (Guillemette et al, 1996; Delos et al, 1998; Negri‐Cesi et al, 1998). Negri‐Cesi et al (1998) have shown that 5α‐reductase‐1 mRNA was present in LNCaP cells, but the 5α‐reductase‐2 mRNA was undetectable, even by RTPCR.…”

Section: Discussionmentioning

confidence: 99%

“…5α‐Reductase‐1 is also expressed in both prostate epithelial and stromal cells (Bruchovsky et al, 1996). 5α‐Reductase activity is clearly demonstrated in prostate cancer tissues and prostatic tumor cells (Bruchovsky et al, 1988; Smith et al, 1994; Delos et al, 1998; Negri‐Cesi et al, 1998). However, the expression pattern of 5α‐reductase isozymes and the importance of 5α‐reductase isozymes in prostate cancer tissues and prostatic tumor cells remain to be further elucidated.…”

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confidence: 99%

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Androgen‐Induced Prostate‐Specific Antigen Gene Expression Is Mediated via Dihydrotestosterone in LNCaP Cells

Zhu

Cai

You

et al. 2003

Journal of Andrology

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Prostate cancer is a leading cause of cancer death in American males. Androgens play an essential role in prostate development, growth and pathogenesis of benign prostate hyperplasia, and prostate cancer. Although testosterone is the main androgen secreted from the testes, dihydrotestosterone (DHT), a more potent androgen converted from testosterone by 5alpha-reductase isozymes, type 1 and 2, is the major androgen in the prostate cells. Thus, 5alpha-reductase(s) are critical in determining androgen activity in the prostate. However, it is unclear in prostate tumor cells whether 1 or 2 5alpha-reductase isozymes are expressed and whether they are functionally important. In the present report, we studied the importance of 5alpha-reductase isozymes in the androgen induction of prostate-specific antigen (PSA) gene expression in LNCaP prostatic tumor cells. Treatment with either testosterone or DHT in LNCaP cells produced dose- and time-dependent increases in PSA levels in the cell media and in PSA messenger RNA (mRNA) levels in the cells. However, testosterone-induced but not DHT-induced PSA gene expression was significantly inhibited by finasteride, a 5alpha-reductase inhibitor, in a dose-dependent manner. Furthermore, we demonstrated for the first time that both 5alpha-reductase-1 and 5alpha-reductase-2 mRNAs were expressed in LNCaP cells using reverse transcriptase-polymerase chain reaction (RT-PCR) and RT-PCR Southern blot analysis. These results suggest that both 5alpha-reductase isozymes are present and functionally important in prostatic tumor LNCaP cells and that DHT is a major mediator of androgen induction of PSA gene expression in these cells.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Androgen‐Induced Prostate‐Specific Antigen Gene Expression Is Mediated via Dihydrotestosterone in LNCaP Cells

Zhu

Cai

You

et al. 2003

Journal of Andrology

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“…Total RNA Table 1. AR, 5α-reductase types 1 and 2 primers were designed according to the published nucleotide sequences for AR [16], 5α-reductase type 1 [17] and 5α-reductase type 2 [18] genes respectively. The expression of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA was examined as an internal control.…”

Section: Rt-pcrmentioning

confidence: 99%

“…In addition, immunoreactive AR protein has been demonstrated to be approx. 7-fold greater in the early [14][15][16][17][18][19][20][21][22] week gestational fetal lung than that of the adult lung [7]. Previous studies [8] have also demonstrated that AR was present in fibroblast cell lines derived from early gestational human fetal lung.…”

Section: Introductionmentioning

confidence: 99%

Expression of androgen receptor and 5α-reductase types 1 and 2 in early gestation fetal lung: a possible correlation with branching morphogenesis

et al. 2003

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The bioactive and potent androgen 5alpha-dihydrotestosterone (DHT) has been postulated to be involved in the development of branching morphogenesis in the human fetal lung, but its expression has not been examined. We therefore examined the expression of the androgen receptor (AR) and 5alpha-reductases (type 1 and type 2), which catalyse the conversion of testosterone into DHT, in the human fetal lung using immunohistochemistry and reverse transcription-PCR (RT-PCR). Immunoreactive AR was detected predominantly in the nuclei of epithelial cells of the budding component of the early gestational fetal lung. 5alpha-Reductase type 1 immunoreactivity was detected in the cytoplasm of epithelial cells, whereas immunoreactivity for 5alpha-reductase type 2 was not detected in the samples of human fetal lung examined. RT-PCR also confirmed the presence of AR and 5alpha-reductase in all fetal lung and epithelial cell lines. The results of our present study suggest that DHT may play an important role in epithelial cells, which might include precursor cells, in which both AR and 5alpha-reductases are expressed during branching morphogenesis of the human fetal lung.

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“…Previous reports have indicated that under expression of the 5␣-R2 isoform is associated with over-expression with 5␣-R1 in undifferentiated AI CaP [33]. Furthermore, Delos et al have shown that over-expression of 5 ␣-R1 is correlated with lack of 5 ␣-R2 expression in AI CaP cell lines [34].…”

Section: Discussionmentioning

confidence: 95%

Molecular profile of androgen-independent prostate cancer xenograft LuCaP 23.1

Fina

Muracciole²,

Rocchi

et al. 2005

The Journal of Steroid Biochemistry and Molecular Biology

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5α-reductase and 17β-hydroxysteroid dehydrogenase expression in epithelial cells from hyperplastic and malignant human prostate

Cited by 16 publications

References 33 publications

Androgen‐Induced Prostate‐Specific Antigen Gene Expression Is Mediated via Dihydrotestosterone in LNCaP Cells

Androgen‐Induced Prostate‐Specific Antigen Gene Expression Is Mediated via Dihydrotestosterone in LNCaP Cells

Expression of androgen receptor and 5α-reductase types 1 and 2 in early gestation fetal lung: a possible correlation with branching morphogenesis

Molecular profile of androgen-independent prostate cancer xenograft LuCaP 23.1

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