5α-Dihydrotestosterone enhances wound healing in diabetic rats

Gonçalves, Reggiani Vilela; Novaes, Rômulo Dias; Sarandy, Mariáurea Matias; Damasceno, E. M.; Matta, Sérgio Luís Pinto da; Gouveia, Neire Moura de; Freitas, Mariella Bontempo; Espíndola, Foued Salmen

doi:10.1016/j.lfs.2016.03.019

Cited by 26 publications

(22 citation statements)

References 42 publications

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“…pseudoquina extract indicate their healing effects. These molecules are bioactive for the treatment of health disorders [ 7 , 43 ], with antioxidant [ 44 , 45 ] and regenerative [ 46 ] abilities, as reported for polyphenols of Dioclea violacea , Erythroxylum numularia [ 47 ], Brassica oleracea [ 48 ] and Bathysa cuspidata [ 31 ]. We believe that plant research is a promising tool for the discovery of extracts or bioactive molecules that can contribute to the treatment of various diseases.…”

Section: Discussionmentioning

confidence: 99%

“…Cytokines and cells can heal cutaneous wounds faster [ 6 , 7 ]. This process includes the inflammatory, proliferative and tissue maturation or remodelling phases [ 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

“…Weak and brittle type III collagen is replaced by strong, tensile-resistant type I collagen in the last stage of the healing process, when fibroblasts, which synthesize the matrix components, predominate and the number of blood vessels decreases [ 18 , 19 ]. Modulating agents stimulate the healing process of cutaneous wounds [ 7 , 20 , 21 ]. Plants stand out in folk medicine [ 22 , 23 ], including Strychnos pseudoquina St. Hil.…”

Section: Introductionmentioning

confidence: 99%

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Strychnos pseudoquina modulates the morphological reorganization of the scar tissue of second intention cutaneous wounds in rats

et al. 2018

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Natural substances are used in folk medicines to treat injuries. Strychnos pseudoquina has scarring, antipyretic, and antimalarial actions. The present study aimed to analyze the effect of S. pseudoquina on cutaneous wound healing in rats. The S. pseudoquina extract was submitted to phytochemical prospection. The levels of flavonoids and total phenolic compounds in the extract were 50.7 mg/g and 2.59 mg/g, respectively. Thirty Wistar rats were individualized in cages with food and water ad libitum (registration no. 730/2014). After anesthesia, three circular wounds (12mm diameter) were made in the animals, which were randomly separated into five treatments: Sal, saline; VO, ointment vehicles (lanolin and Vaseline); SS, positive control (silver sulfadiazine 1%); LE 5, freeze-dried extract 5%; and LE 10, lyophilized extract 10%. The animals were treated with the ointment daily for 21 days. Every seven days, the area and the rate of wound contraction were evaluated. Tissue samples were removed for histopathological analysis of the number of mast cells, elastic and collagen fibers, and biochemical analyses, quantification of malondialdehyde (MDA), carbonylated proteins (PCN), superoxide dismutase (SOD), catalase (CAT), transforming growth factor β (TGF-β), Interleukin 10 (IL-10) and tumor necrosis factor (TNF). The number of mast cells, collagen and elastic fibers in the rat wounds were higher in the treatments with the plant. The extract also stimulated the activity of antioxidant enzymes, particularly SOD, presenting high levels, and maintained low levels of PCN. The TGF-β and IL-10 concentration was higher in the LE5 and LE10 treatment of the extract than in the Sal, OV and SS treatments on day 7. The ointment based on S. pseudoquina closed the wound faster and accelerated wound healing in animals.

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Section: Discussionmentioning

confidence: 99%

“…Cytokines and cells can heal cutaneous wounds faster [ 6 , 7 ]. This process includes the inflammatory, proliferative and tissue maturation or remodelling phases [ 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Strychnos pseudoquina modulates the morphological reorganization of the scar tissue of second intention cutaneous wounds in rats

et al. 2018

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“…Lipid peroxidation is an important pathophysiological event in several diseases36. MDA from lipid peroxidation can interact with DNA bases and induce mutagenic lesions37. Pratibha et al 38.…”

Section: Discussionmentioning

confidence: 99%

In vivo Assessment of Antioxidant and Wound Healing Improvement of a New Schiff Base Derived Co (II) Complex in Rats

El-Ferjani

Ahmad

Dhiyaaldeen

et al. 2016

Sci Rep

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Co (II) complex (CMLA) was investigated to evaluate the rate of wound healing in rats. Animals were placed into four groups: gum acacia, Intrasite gel, 10 and 20 mg/ml of CMLA. Wounds were made on the dorsal neck area, then treated with Intrasite gel or CMLA; both of these treatments led to faster healing than with gum acacia. Histology of the wounds dressed with CMLA or Intrasite gel displayed a smaller scar width, required less time to heal and showed more collagen staining and fewer inflammatory cells in comparison to wounds dressed with the vehicle. Immunohistochemistry for Hsp70 and TGF-β showed greater staining intensity in the treated groups compared to the vehicle group. Bax staining was less intense in treated groups compared to the vehicle group, suggesting that CMLA and Intrasite gel provoked apoptosis, responsible for the development of granulation tissue into a scar. CD31 protein analysis showed that the treated groups enhanced angiogenesis and increased vascularization compared to the control group. Furthermore, a significant increase in the levels of GPx and SOD and a decrease in MDA were also observed in the treated groups. This results suggest that CMLA is a potentially promising agent for the wounds treatment.

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“…Several studies suggest that testosterone inhibits human wound healing, and testosterone has been reported to contribute to a slower barrier development in the fetus (Fimmel and Zouboulis, 2005;Greenough, 1996). A single report on STZ-induced diabetic rats suggested that 5a-dihydrotestosterone (i.e., DTH) improved wound healing compared with sham-treated wounds (Goncalves et al, 2016). Unfortunately, the authors did not clarify the sex of the animals used.…”

Section: Microbiome and Murine Wound Modelsmentioning

confidence: 99%

A Modeling Conundrum: Murine Models for Cutaneous Wound Healing

Elliot

Wikramanayake

Jozic

et al. 2018

Journal of Investigative Dermatology

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The complexity of the cutaneous wound healing process and its impairment in disease states, combined with the urgent clinical need for new therapies demand well-defined preclinical models that facilitate translation of research findings to clinical use. Many murine wound models are well suited for studying mechanisms of various aspects of wound healing, but they have shown limited utility for translating research findings to human conditions, thereby impeding therapeutic developments. Ansell et al. underscore the need for standardization of preclinical murine models by showing the impact of duration of diabetes, animal sex, and assessment methods on healing outcomes in the streptozotocin-induced diabetes mellitus rat model. Recognizing and understanding the limitations and advantages of preclinical murine wound models will facilitate more effective translation of experimental results to improved treatment of human chronic wounds.

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5α-Dihydrotestosterone enhances wound healing in diabetic rats

Cited by 26 publications

References 42 publications

Strychnos pseudoquina modulates the morphological reorganization of the scar tissue of second intention cutaneous wounds in rats

Strychnos pseudoquina modulates the morphological reorganization of the scar tissue of second intention cutaneous wounds in rats

In vivo Assessment of Antioxidant and Wound Healing Improvement of a New Schiff Base Derived Co (II) Complex in Rats

A Modeling Conundrum: Murine Models for Cutaneous Wound Healing

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