5S rDNA organization in the fish Synbranchus marmoratus (Synbranchidae, Synbranchiformes)

Messias, Luciano Henrique Vieira; Ferreira, Daniela Cristina; Wasko, Adriane Pinto; Oliveira, Cláudio; Foresti, Fausto; Martins, Cesar

doi:10.1111/j.1601-5223.2003.01759.x

Cited by 16 publications

(15 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…G. cuvier and A. superciliosus) could either carry two classes of a similar mass or one single class, although more than PCR data are necessary to test these hypotheses. The loss of a cluster might have occurred secondarily in fish taxa that bear only one class of 5S rDNA, as reported for Synbranchus marmoratus (Messias et al, 2003), Acipenser sturio (Tagliavini et al, 1999) and G. aculeatus (Rocco et al, 1999), perhaps caused by a strong reduction of the NTS (less than 60-80 bp length) below the minimum required to sustain the array and the dynamics of the 5S rDNA in the genome (Martins & Galetti, 2001b). To date, the short-class array length of 5S rDNA in the R. lalandii and R. porosus genomes has always been found to be greater than the apparently critical 60 bp length (Table 1), the same length as found for G. cuvier and A. superciliosus (data not shown but available upon request).…”

Section: Ii) Nts Featuresmentioning

confidence: 98%

“…The intense dynamism of 5S rDNA repeats generates variant classes of 5S rDNA, which have been reported in the genomes of several vertebrates, from lampreys to mammals (Komiya et al, 1986 ;Hallenberg et al, 1994;Frederiksen et al, 1997). Particularly in marine and freshwater ray-finned fishes, vast numbers of structural and functional data have demonstrated the occurrence of a dual size-class pattern of organization of the 5S rDNA (Moran et al, 1996 ;Ce´spedes et al, 1999 ;Rocco et al, 1999;Deiana et al, 2000 ;Martins & Galletti, 2001 a ;Wasko et al, 2001 ;Martins et al, 2002 ;Messias et al, 2003 ;Tigano et al, 2004 ;Alves-Costa et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Molecular organization of 5S rDNA in sharks of the genusRhizoprionodon: insights into the evolutionary dynamics of 5S rDNA in vertebrate genomes

Pinhal¹,

Araki²,

Gadig³

et al. 2009

Genet. Res.

Self Cite

View full text Add to dashboard Cite

SummaryIn this study, we attempted a molecular characterization of the 5S rDNA in two closely related species of carcharhiniform sharks, Rhizoprionodon lalandii and Rhizoprionodon porosus, as well as a further comparative analysis of available data on lampreys, several fish groups and other vertebrates. Our data show that Rhizoprionodon sharks carry two 5S rDNA classes in their genomes : a short repeat class (termed class I) composed of y185 bp repeats, and a large repeat class (termed class II) arrayed in y465 bp units. These classes were differentiated by several base substitutions in the 5S coding region and by completely distinct non-transcribed spacers (NTS). In class II, both species showed a similar composition for both the gene coding region and the NTS region. In contrast, class I varied extensively both within and between the two shark species. A comparative analysis of 5S rRNA gene sequences of elasmobranchs and other vertebrates showed that class I is closely related to the bony fishes, whereas the class II gene formed a separate cartilaginous clade. The presence of two variant classes of 5S rDNA in sharks likely maintains the tendency for dual ribosomal classes observed in other fish species. The present data regarding the 5S rDNA organization provide insights into the dynamics and evolution of this multigene family in the fish genome, and they may also be useful in clarifying aspects of vertebrate genome evolution.

show abstract

Section: Ii) Nts Featuresmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Molecular organization of 5S rDNA in sharks of the genusRhizoprionodon: insights into the evolutionary dynamics of 5S rDNA in vertebrate genomes

Pinhal¹,

Araki²,

Gadig³

et al. 2009

Genet. Res.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Different classes of the 5S rRNA gene, identified in many fish species, varied by length, several nucleotide mutations or chromosomal location (Messias et al, 2003;Pinhal et al, 2008;Campo, Machado-Schiaffino, Horreo, & Garcia-Vazquez, 2009;He et al, 2012He et al, , 2013. Multi-class type 5S rDNA are considered an ancestral character that originated early in the history of vertebrates (Frederiksen, Cao, Lomholt, Levan, & Hallenberg, 1997).…”

Section: Discussionmentioning

confidence: 99%

“…Multi-class type 5S rDNA are considered an ancestral character that originated early in the history of vertebrates (Frederiksen, Cao, Lomholt, Levan, & Hallenberg, 1997). However, not all ancient groups of fish exhibit different types of minor rDNA (Messias et al, 2003). The presence of at least the two different classes (340 and 470 bp) of 5S rRNA gene in the Prussian carp may suggest that its genome retained some ancestral …”

Section: Discussionmentioning

confidence: 99%

“…In some fish species, different classes of this gene have been described. Each of the different-sized 5S rDNA classes vary in length, nucleotide changes and chromosome location (Martins, 2006;Messias et al, 2003;Pinhal et al, 2008;Campo, MachadoSchiaffino, Horreo, & Garcia-Vazquez, 2009). Most fish species are characterized by the occurrence of two 5S rDNA arrays with a different NTS (Martins & Galetti Jr., 2001;He et al, 2012;Qin, Wang, Wang, Liu, & Liu, 2015).…”

Section: Aleksandra Szabelskamentioning

confidence: 99%

See 1 more Smart Citation

Untitled

Szabelska¹,

Kirtiklis²,

Przybył³

et al. 2017

Turk. J. Fish. Aquat. Sci.

View full text Add to dashboard Cite

The Prussian carp may occur as diploid (2n=100) and/or triploid (3n=150) individuals, co-existing in many natural populations. The simultaneous occurrence of individuals with different ploidy makes the taxonomy of this species unclear. Additionally, the taxonomic status of C. gibelio has become even more enigmatic due to its hybridizing with other nonindigenous cyprinids. Since the variation within 5S rDNA can serve as a suitable marker for molecular identification of the fish ploidy, the main aim of present study was to compare this rDNA sequence between Prussian carp individuals with different ploidy levels: diploids (2n =100) and triploids (3n=150-160). PCR amplification of 5S rDNA generated two bands of approximately 340 and 470 bp in length (in both diploids and triploids) and band 200 bp visible in some individuals. These results indicate the presence of at least two different classes of 5S rRNA gene. Analysis of their nucleotide composition revealed no differences within the class of 340 bp and several nucleotide differences within the class of 470 bp between diploid and triploid individuals. The 5S rDNA variability detected in this study indicates the potential usefulness of this sequence for the identification of diploid and triploid individuals of the Prussian carp.

show abstract

Molecular Organization of 5S rDNAs in Rajidae (Chondrichthyes): Structural Features and Evolution of Piscine 5S rRNA Genes and Nontranscribed Intergenic Spacers

et al. 2006

View full text Add to dashboard Cite

The genomic and gene organisation of 5S rDNA clusters have been extensively characterized in bony fish and eukaryotes, providing general issues for understanding the molecular evolution of this multigene DNA family. By contrast, the 5S rDNA features have been rarely investigated in cartilaginous fish (only three species). Here, we provide evidence for a dual 5S rDNA gene system in the Rajidae by sequence analysis of the coding region (5S) and adjacent nontranscribed spacer (NTS) in five Mediterranean species of rays (Rajidae), and in a large number of piscine taxa including lampreys and bony fish. As documented in several bony fish, two functional 5S rDNA types were found here also in the rajid genome: a short one (I) and a long one (II), distinguished by distinct 5S and NTS sequences. That the ancestral piscine genome had these two 5S rDNA loci might be argued from the occurrence of homologous dual gene systems that exist in several fish taxa and from 5S phylogenetic relationships. An extensive analysis of NTS-II sequences of Rajidae and Dasyatidae revealed the occurrence of large simple sequence repeat (SSR) regions that are formed by microsatellite arrays. The localization and organization of SSR within the NTS-II are conserved in Rajiformes since the Upper Cretaceous. The direct correlation between the SSRs extension and the NTS length indicated that they might play a role in the maintenance of the larger 5S rDNA clusters in rays. The phylogenetic analysis indicated that NTS-II is a valuable systematic tool limited to distantly related taxa of Rajiformes.

show abstract

5S rDNA organization in the fish Synbranchus marmoratus (Synbranchidae, Synbranchiformes)

Cited by 16 publications

References 22 publications

Molecular organization of 5S rDNA in sharks of the genusRhizoprionodon: insights into the evolutionary dynamics of 5S rDNA in vertebrate genomes

Molecular organization of 5S rDNA in sharks of the genusRhizoprionodon: insights into the evolutionary dynamics of 5S rDNA in vertebrate genomes

Untitled

Molecular Organization of 5S rDNAs in Rajidae (Chondrichthyes): Structural Features and Evolution of Piscine 5S rRNA Genes and Nontranscribed Intergenic Spacers

Contact Info

Product

Resources

About