Summarywith copper hydroxide or trichloroacetic acid and protein calculated from amino acid nitrogen. Protein content increase was likely, resulting from a significant increase of aspartic acid, arginine, glutamic acid, leucine, lysine and valine contents. Chitin content unfortunately increased significantly in Pleurorus mushroom grown on grass hay substrate. No significant difference was recorded in the case of acid detergent fibre, lipids, minerals and vitamins. In contrast, neutral detergent fibre, acid detergent lignin and sugar contents decreased in Pleurotus grown on grass hay.Fruitbodies of I! ostrearus var: columbinus harvested from grass hay and wheat straw substrates were analyzed and compared in order to assess changes of their nutritional status depending upon cultivation substrate. The results showed that grass hay substrate increased significantly the protein content expressed as crude protein, digestible protein, protein precipitated