599 A comparison of environmental benzene exposure in subjects from rural and urban populations using an immunoassay for the specific biomarker S-phenylmercapturic acid (S-PMA)

Comparison of hydrolysis and HPLC/MS/MS procedure with ELISA assay for the determination of S-phenylmercapturic acid as a biomarker of benzene exposure in human urine

“…Two 300-L samples, one the detection reagent 1 (0.5% hydrogen peroxide and 0.1 M nitric acid) and the other detection reagent 2 (0.025 M sodium hydroxide and 0.2% surfactant), were added to the tubes before the measurement [15,16]. The performance characteristics of this method, determined by the manufacturer, were as follows:…”