588 Features of Conduct and Treatment of Resistant Epileptic Attacks in Children of Early Age with a Tuberous Sclerosis

Abstract

Aims:1) to quantify the tissue UCB content (TUCB) spontaneously present in five brain regions of Gunn rats; and 2) to unravel the dynamics of TUCB entry and clearance after sulphadimethoxine treatment .Methods: Cortex (Cx), superior collicula (SC), inferior collicula (IC), cerebellum (CLL) and the rest of the brain (RB) were collected 1, 6, 24, 48 and 72 hrs after injection of sulfadimethoxine (200 mg/Kg; IP) in 17 days-old jj animals and in not treated JJ, Jj and jj rats. TUCB was analyzed by the HPLC based method (Zelenska_2008). Results:In untreated jj animals TUCB brain amounts was about 80 times higher than in Jj and JJ, and no regional differences were detected. After sulphadimethoxine administration TUCB picked differently in the regions analyzed: 1 hr in Cx and SC, 6 hrs in RB, 24 hrs in IC, 6 -24 hrs in CLL. 72 hrs after injection, TUCB content was lower than in not treated littermates. Conclusions:The differential entry and clearance across brain areas show regional difference in cellular mechanisms modulating tissue UCB concentration. The different kinetics might be involved in the tissue resistance/sensitivity to bilirubin toxicity of nervous system. AMPLITUDE INTEGRATED EEG AS