58,000 Dalton Intermediate Filament Proteins of Neuronal and Nonneuronal Origin in the Goldfish Visual Pathway

Quitschke, Wolfgang; Schechter, Nisson

doi:10.1111/j.1471-4159.1984.tb02715.x

Cited by 57 publications

(70 citation statements)

References 48 publications

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“…lA), as previously reported (Nona et al, 1989;Quitschke and Schechter, 1984;Quitschke et al, 1980). Two major protein bands were apparent at 48 kDa and 58 kDa, while the presence of a 51 kDa band, corresponding to GFAP, was not readily recognisable.…”

Section: Normal Goldfishsupporting

confidence: 75%

Expression of glial fibrillary acidic protein (GFAP) in goldfish optic nerve following injury

Stafford¹,

Shehab²,

Nona³

et al. 1990

Glia

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By using an antibody to goldfish glial fibrillary acidic protein (GFAP), the reaction of goldfish optic nerve to injury has been studied by immunoblotting and immunohistochemical methods. Goldfish optic nerve, which normally lacks GFAP immunoreactivity (Nona et al.: Glia, 2:189-200, 1989), expresses GFAP following injury. This immunoreactivity, which is observed as early as 10 days after crush and which is still evident at 30 days after crush, all but disappears by 150 days after crush. Since it is well established that functional restoration of synaptic connections and the recovery of vision takes place in goldfish following optic nerve injury, our results indicate that reactive astrocytes do not represent an impediment to regeneration in goldfish visual system.

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Section: Normal Goldfishsupporting

confidence: 75%

Expression of glial fibrillary acidic protein (GFAP) in goldfish optic nerve following injury

Stafford¹,

Shehab²,

Nona³

et al. 1990

Glia

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“…5b), not glia. Bands 2 and 3 are probably equivalent to the 58 kDa complex of axonal and glial proteins found in goldfish optic nerve (Quitschke and Schechter, 1984), and in agreement, Ab2 and Ab3 recognized optic axons and astroglia, respectively, in the goldfish.…”

Section: Anatomical Synopsissupporting

confidence: 75%

Glial domains and nerve fiber patterns in the fish retinotectal pathway

Maggs¹,

Scholes²

1986

J. Neurosci.

129

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Optic nerve fibers run parallel from the retina as far as the optic tract in fish, then suddenly criss-cross into a new pattern matching the tectal map. This change coincides with a unique demarcation between two astroglial territories in the retinotectal pathway, located where the optic chiasm occurs in other vertebrates, which we defined using antibodies directed against intermediate filaments (IF). We found that astroglia in optic nerve territory express an Mr 56,000 IF polypeptide, band 3, which we identify as the fish equivalent of vimentin in mammals. These astrocytic cells lack glial fibrillary acidic protein (GFAP; cf. Dahl and Bignami, 1973). Conversely, glia in brain territory, that is, in the optic tract and elsewhere in the CNS, lack the fish vimentin, but express GFAP. By electron microscopy, we obtained evidence that new retinal axons extend swiftly through the growing optic nerve, where they are tightly shepherded into a narrow track by newly differentiating glial cells, positive for the fish vimentin. In the GFAP-positive glial territory of the optic tract, by contrast, growing axons are slowed down and probably branch. We suggest that this allows them to fasciculate accurately with older fibers and thereby propagate a tectotopic pattern established by pioneer axons in the embryo.

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“…Previous biochemical (Quitschke and Schechter, 1984) and histological (Jones et al, 198613) results showed that gefiltin is the predominant neurofilament protein in the goldfish optic nerve, whereas plasticin was a minor neurofilament protein. Both were synthesized in ganglion cells and transported into the optic nerve within the slow phase of axonal transport (Quitschke and Schechter, 1984;Glasgow et al, 1992).…”

mentioning

confidence: 97%

“…Both were synthesized in ganglion cells and transported into the optic nerve within the slow phase of axonal transport (Quitschke and Schechter, 1984;Glasgow et al, 1992). During regeneration following optic nerve crush, the rates of mRNA accumulation for gefiltin…”

mentioning

confidence: 99%

Plasticin, a newly identified neurofilament protein, is preferentially expressed in young retinal ganglion cells of adult goldfish

Fuchs

Glasgow

Hitchcock

et al. 1994

J of Comparative Neurology

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The adult goldfish retina and optic nerve display continuous growth, plasticity, and the capacity to regenerate throughout the animal's life. The intermediate filament proteins in this pathway are different from those in adult mammalian nerves, which do not continuously grow or normally regenerate. One novel intermediate filament protein of the goldfish visual pathway is plasticin, which is synthesized in ganglion cells and transported into the optic nerve. Using specific polyclonal antibodies raised against a plasticin fusion protein, we investigated the distribution of this protein in the normal retina and nerve and in the retina and nerve following optic nerve crush. In the normal pathway, plasticin was localized predominantly to the axons of very young ganglion cells; however, there was considerable immunoreactivity in older axons as they approach the chiasm. In addition, following optic nerve crush, all ganglion cell somata and their axons proximal to the crush site became equally immunoreactive. The results suggest that plasticin may contribute to axonal growth, plasticity, and regeneration.

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58,000 Dalton Intermediate Filament Proteins of Neuronal and Nonneuronal Origin in the Goldfish Visual Pathway

Cited by 57 publications

References 48 publications

Expression of glial fibrillary acidic protein (GFAP) in goldfish optic nerve following injury

Expression of glial fibrillary acidic protein (GFAP) in goldfish optic nerve following injury

Glial domains and nerve fiber patterns in the fish retinotectal pathway

Plasticin, a newly identified neurofilament protein, is preferentially expressed in young retinal ganglion cells of adult goldfish

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