579 Cytotoxic effects of resveratrol on imatinib sensitive and resistant K562 chronic myeloid leukemia cells

Can, Geylani; Baran, Yusuf

doi:10.1016/s1359-6349(10)71380-x

Cited by 13 publications

(16 citation statements)

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“…We previously demonstrated activation of caspase-3 enzyme activity in response to resveratrol in imatinib-sensitive and -resistant chronic myeloid leukemia and acute promyelocytic leukemia cells. 27 Estrov et al applied resveratrol and two caspase inhibitors on OCIM2 cells and reported that both caspase inhibitors partially reversed the antiproliferative effect of resveratrol.…”

Section: Discussionmentioning

confidence: 99%

Resveratrol and quercetin-induced apoptosis of human 232B4 chronic lymphocytic leukemia cells by activation of caspase-3 and cell cycle arrest

2013

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Chronic lymphocytic leukemia (CLL), defined by accumulation of pathogenic B cells, has a very complex biology due to various factors such as inherited, host, and enviromental factors. Recently, finding new therapeutic agents or development of novel treatment strategies have been paid attention. Resveratrol and quercetin, important phytoalexins found in many plants, have been reported to have cytotoxic effects on various types of cancer. In this study, we examined cytotoxic, cytostatic, and apoptotic effects of these two important phenolic compounds on 232B4 human CLL cells. Cytotoxic effects of resveratrol and quercetin were determined by MTT cell proliferation assay. Changes in caspase-3 enzyme activity were measured using caspase-3 colorimetric assay. Annexin V-FITC/PI double staining was performed to measure apoptotic cell population. Effects of resveratrol and quercetin on cell cycle profiles of CLL cells were investigated by flow cytometry. Treatment of CLL cells with resveratrol and quercetin caused dose dependent inhibition of cell proliferation and increased apoptotic cell population through induction of caspase-3 activity. Cell cycle analysis displayed cell cycle arrest mainly in G0/G1 for both polyphenols. Our data, in total, showed for the first time that resveratrol and quercetin might block CLL growth through inducing apoptosis and cell cycle arrest.

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Section: Discussionmentioning

confidence: 99%

Resveratrol and quercetin-induced apoptosis of human 232B4 chronic lymphocytic leukemia cells by activation of caspase-3 and cell cycle arrest

2013

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“…Phytoconstituents like apigenin, resveratrol etc. that are found in wide range of fruits and vegetables and are gaining prominence owing to its ability to induce apoptosis via loss of mitochondrial membrane potential and caspase activation in K562 sensitive and K562/IMA3 (K562 cell resistant to 3uM imatinib mesylate) cells [ 240 – 243 ]. In addition to this and above mentioned natural compounds like fiestin, hesperidin, virosecurinine, cryptotanshinone, quercetin, genistein vincristine, and many more had competence when used alone or in combination with other tyrosine kinase inhibitor to down regulate BCR/ABL and lyn levels in cells developing resistance to imatinib mesylate and CML patients [ 244 – 249 ].…”

Section: Targeting Nrtks Using Natural Productsmentioning

confidence: 99%

Role of Non Receptor Tyrosine Kinases in Hematological Malignances and its Targeting by Natural Products

et al. 2018

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Tyrosine kinases belong to a family of enzymes that mediate the movement of the phosphate group to tyrosine residues of target protein, thus transmitting signals from the cell surface to cytoplasmic proteins and the nucleus to regulate physiological processes. Non-receptor tyrosine kinases (NRTK) are a sub-group of tyrosine kinases, which can relay intracellular signals originating from extracellular receptor. NRTKs can regulate a huge array of cellular functions such as cell survival, division/propagation and adhesion, gene expression, immune response, etc. NRTKs exhibit considerable variability in their structural make up, having a shared kinase domain and commonly possessing many other domains such as SH2, SH3 which are protein-protein interacting domains. Recent studies show that NRTKs are mutated in several hematological malignancies, including lymphomas, leukemias and myelomas, leading to aberrant activation. It can be due to point mutations which are intragenic changes or by fusion of genes leading to chromosome translocation. Mutations that lead to constitutive kinase activity result in the formation of oncogenes, such as Abl, Fes, Src, etc. Therefore, specific kinase inhibitors have been sought after to target mutated kinases. A number of compounds have since been discovered, which have shown to inhibit the activity of NRTKs, which are remarkably well tolerated. This review covers the role of various NRTKs in the development of hematological cancers, including their deregulation, genetic alterations, aberrant activation and associated mutations. In addition, it also looks at the recent advances in the development of novel natural compounds that can target NRTKs and perhaps in combination with other forms of therapy can show great promise for the treatment of hematological malignancies.

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“…The antiproliferative and proapoptotic activity of RSV has been reported in different human cancer cell lines that include colon, prostate, and breast cancer as well as leukemia [ 12 , 13 , 14 , 15 ]. Although there is abundant data regarding the chemopreventive role of RSV in many cancers, relatively little information exists on the antiproliferative activity of RSV in human lymphoma cells [ 16 , 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

The Effect of Resveratrol on Cell Viability in the Burkitt’s Lymphoma Cell Line Ramos

et al. 2017

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Resveratrol is a polyphenolic natural compound produced by a variety of crops. Currently, resveratrol is considered a multi-target anti-cancer agent with pleiotropic activity, including the ability to prevent the proliferation of malignant cells by inhibiting angiogenesis and curtailing invasive and metastatic factors in many cancer models. However, the molecular mechanisms mediating resveratrol-specific effects on lymphoma cells remain unknown. To begin tackling this question, we treated the Burkitt’s lymphoma cell line Ramos with resveratrol and assessed cell survival and gene expression. Our results suggest that resveratrol shows a significant anti-proliferative and pro-apoptotic activity on Ramos cells, inducing the DNA damage response, DNA repairing, and modulating the expression of several genes that regulate the apoptotic process and their proliferative activity.

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579 Cytotoxic effects of resveratrol on imatinib sensitive and resistant K562 chronic myeloid leukemia cells

Cited by 13 publications

References 0 publications

Resveratrol and quercetin-induced apoptosis of human 232B4 chronic lymphocytic leukemia cells by activation of caspase-3 and cell cycle arrest

Resveratrol and quercetin-induced apoptosis of human 232B4 chronic lymphocytic leukemia cells by activation of caspase-3 and cell cycle arrest

Role of Non Receptor Tyrosine Kinases in Hematological Malignances and its Targeting by Natural Products

The Effect of Resveratrol on Cell Viability in the Burkitt’s Lymphoma Cell Line Ramos

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