561 Prostate calcifications and LUTS: The potential role of bacterial biofilm and prostate inflammation

Cai, Tai-sheng; Tiscione, D.; Caola, Iole; Piccoli, Federico; Tessarolo, Francesco; Nollo, Giandomenico; Mazzoli, Sandra; Lanzafame, Paolo; Malossini, G.; Caciagli, Patrizio; Bartoletti, R.

doi:10.1016/s1569-9056(14)60552-2

Cited by 2 publications

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“…It is well known that not all patients with sonographic demonstration of prostate calcifications report clinical symptoms, but several authors have demonstrated that the presence and grade of prostate calcifications is associated with worsening of symptoms [ 4 , 5 , 7 ]. Moreover, some authors have reported that prostate calcifications are associated with prostate inflammation and symptoms [ 15 , 24 , 25 ]. Even if the natural history of prostate calcification is still not completely understood, we have added new data suggesting that bacterial biofilms might play a key role in the genesis of prostate calcifications and in the persistence of symptoms in a non-negligible fraction of antibiotic-treated patients.…”

Section: Discussionmentioning

confidence: 99%

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Prostate calcifications: A case series supporting the microbial biofilm theory

et al. 2018

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PurposeProstate calcifications are a common finding during transrectal prostate ultrasound in both healthy subjects and patients, but their etiopathogenesis and clinical significance are not fully understood. We aimed to establish a new methodology for evaluating the role of microbial biofilms in the genesis of prostate calcifications.Materials and MethodsTen consecutive patients who had undergone radical prostatectomy were enrolled in this study. All of the patients presented with prostate calcifications during transrectal ultrasound evaluation before surgery and underwent Meares-Stamey tests and clinical evaluation with the National Institutes of Health Chronic Prostatitis Symptom Index and the International Prostate Symptom Score. At the time of radical prostatectomy, the prostate specimen, after removal, was analyzed with ultrasonography under sterile conditions in the operating room. Core biopsy specimens were taken from the site of prostate calcification and subjected to ultrastructural and microbiological analysis.ResultsThe results of the Meares-Stamey test showed only 1 of 10 patients (10%) with positive cultures for Escherichia coli. Two of five patients (40%) had positive cultures from prostate biopsy specimens. Enterococcus faecalis, Enterococcus raffinosus, and Citrobacter freundii were isolated. Ultrastructural analysis of the prostate biopsy specimens showed prostate calcifications in 6 of 10 patients (60%), and a structured microbial biofilm in 1 patient who had positive cultures for E. faecalis and E. raffinosus.ConclusionsAlthough the findings are supported by a low number of patients, this study highlights the validity of the proposed methodology for investigating the role of bacterial biofilms in the genesis of prostate calcification.

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Section: Discussionmentioning

confidence: 99%

“…Any micromorphology compatible with microbial biofilm or tissue calcification was inspected and documented by collecting a set of digital micrographs, in line with Cai et al [ 14 ]. The crystal composition of calcifications was assessed by using the energy-dispersive X-ray spectroscope integrated in the SEM, in line with Cai et al [ 15 ].…”

Section: Methodsmentioning

confidence: 99%

Prostate calcifications: A case series supporting the microbial biofilm theory

et al. 2018

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“…micromorphology compatible with microbial biofilm or tissue calcification was inspected and documented by collecting a set of digital micrographs, in line with Cai et al [14]. The crystal composition of calcifications was assessed by using the energy-dispersive X-ray spectroscope integrated in the SEM, in line with Cai et al [15].…”

Section: Prostate Calcifications and Bacterial Biofilmsmentioning

confidence: 99%

Prostate calcifications: A case series supporting the microbial biofilm theory

Puglisi¹,

Cai²,

Caciagli³

et al. 2019

European Urology Supplements

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Prostate calcifications are a common finding during transrectal prostate ultrasound in both healthy subjects and patients, but their etiopathogenesis and clinical significance are not fully understood. We aimed to establish a new methodology for evaluating the role of microbial biofilms in the genesis of prostate calcifications. Materials and Methods: Ten consecutive patients who had undergone radical prostatectomy were enrolled in this study. All of the patients presented with prostate calcifications during transrectal ultrasound evaluation before surgery and underwent Meares-Stamey tests and clinical evaluation with the National Institutes of Health Chronic Prostatitis Symptom Index and the International Prostate Symptom Score. At the time of radical prostatectomy, the prostate specimen, after removal, was analyzed with ultrasonography under sterile conditions in the operating room. Core biopsy specimens were taken from the site of prostate calcification and subjected to ultrastructural and microbiological analysis. Results: The results of the Meares-Stamey test showed only 1 of 10 patients (10%) with positive cultures for Escherichia coli. Two of five patients (40%) had positive cultures from prostate biopsy specimens. Enterococcus faecalis, Enterococcus raffinosus, and Citrobacter freundii were isolated. Ultrastructural analysis of the prostate biopsy specimens showed prostate calcifications in 6 of 10 patients (60%), and a structured microbial biofilm in 1 patient who had positive cultures for E. faecalis and E. raffinosus. Conclusions: Although the findings are supported by a low number of patients, this study highlights the validity of the proposed methodology for investigating the role of bacterial biofilms in the genesis of prostate calcification.

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561 Prostate calcifications and LUTS: The potential role of bacterial biofilm and prostate inflammation

Cited by 2 publications

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Prostate calcifications: A case series supporting the microbial biofilm theory

Prostate calcifications: A case series supporting the microbial biofilm theory

Prostate calcifications: A case series supporting the microbial biofilm theory

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