546 Quantifying Human Eosinophils Using 3-Dimensional Volumetric Images Collected With Multi-Photon Fluorescence Microscopy

Safdarian, Nastaran; Liu, Zhongyao; Zhou, Xiaoming; Appelman, Henry D.; Nostrant, Timothy T.; Wang, Thomas D.; Wang, Emily T.

doi:10.1016/s0016-5085(12)60411-9

Cited by 2 publications

(1 citation statement)

References 16 publications

(17 reference statements)

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“…Because of these challenges and the somewhat rudimentary way in which EoE is diagnosed, there is active research aimed at identifying better ways to diagnose EoE. Some methods under investigation include clinical scoring systems, 8,78,79 endoscopic imaging techniques, 56,80,81 functional luminal assessment of the esophagus, 82 biomarker measurements on esophageal biopsies, [83][84][85][86][87] radio-labelling of esophageal biopsies, 88 noninvasive assessment of esophageal cytokines, 89 non-invasive serum biomarkers, [90][91][92] and genetic testing. 21,93 While these are promising, none of these techniques have been validated or are ready to be used in clinical practice.…”

Section: Diagnostic Dilemmasmentioning

confidence: 99%

Eosinophilic Esophagitis

Hirano¹,

Dellon²

2021

The Esophagus

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Eosinophilic esophagitis (EoE) is a chronic immune-mediated condition where infiltration of eosinophils into the esophageal mucosa leads to symptoms of esophageal dysfunction. It has rapidly emerged as an important cause of upper GI morbidity in patients of all ages and is encountered in a substantial proportion of patients undergoing diagnostic upper endoscopy. This review discusses the clinical, endoscopic, and histologic features of EoE and presents the most recent guidelines for diagnosis of EoE. It describes selected diagnostic dilemmas including distinguishing EoE from gastroesophageal reflux disease and addressing the newly recognized clinical entity of proton pump inhibitor responsive esophageal eosinophilia. It also highlights evidence to support both pharmacologic and non-pharmacologic treatments, including topical corticosteroids, dietary elimination therapy, and endoscopic dilation.

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Section: Diagnostic Dilemmasmentioning

confidence: 99%

Eosinophilic Esophagitis

Hirano¹,

Dellon²

2021

The Esophagus

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Imaging of Mucosal Inflammation: Current Technological Developments, Clinical Implications, and Future Perspectives

et al. 2017

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In recent years, various technological developments markedly improved imaging of mucosal inflammation in patients with inflammatory bowel diseases. Although technological developments such as high-definition-, chromo-, and autofluorescence-endoscopy led to a more precise and detailed assessment of mucosal inflammation during wide-field endoscopy, probe-based and stationary confocal laser microscopy enabled in vivo real-time microscopic imaging of mucosal surfaces within the gastrointestinal tract. Through the use of fluorochromes with specificity against a defined molecular target combined with endoscopic techniques that allow ultrastructural resolution, molecular imaging enables in vivo visualization of single molecules or receptors during endoscopy. Molecular imaging has therefore greatly expanded the clinical utility and applications of modern innovative endoscopy, which include the diagnosis, surveillance, and treatment of disease as well as the prediction of the therapeutic response of individual patients. Furthermore, non-invasive imaging techniques such as computed tomography, magnetic resonance imaging, scintigraphy, and ultrasound provide helpful information as supplement to invasive endoscopic procedures. In this review, we provide an overview on the current status of advanced imaging technologies for the clinical non-invasive and endoscopic evaluation of mucosal inflammation. Furthermore, the value of novel methods such as multiphoton microscopy, optoacoustics, and optical coherence tomography and their possible future implementation into clinical diagnosis and evaluation of mucosal inflammation will be discussed.

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546 Quantifying Human Eosinophils Using 3-Dimensional Volumetric Images Collected With Multi-Photon Fluorescence Microscopy

Cited by 2 publications

References 16 publications

Eosinophilic Esophagitis

Eosinophilic Esophagitis

Imaging of Mucosal Inflammation: Current Technological Developments, Clinical Implications, and Future Perspectives

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