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Tidona, Christian A.; Schnitzler, Paul; Kehm, Roland; Darai, Gholamreza

doi:10.1023/a:1007949710031

Cited by 155 publications

(36 citation statements)

References 29 publications

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“…MCP gene contains highly conserved domains and codes for the major structural component of viral particles [45][46][47][48]. The MCP gene has been used to detect and measure RSIV as well as other iridoviruses [14,48,49]. Thus, our results further confirm the hypothesis that the spleen is the target organ for RSIV infection.…”

Section: Discussionsupporting

confidence: 79%

“…Among the selected ORFs, the MCP gene was found to be expressed at higher levels in the spleen than in the kidney over the time-course of infection. MCP gene contains highly conserved domains and codes for the major structural component of viral particles [45][46][47][48]. The MCP gene has been used to detect and measure RSIV as well as other iridoviruses [14,48,49].…”

Section: Discussionmentioning

confidence: 99%

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Transcriptional profile of red seabream iridovirus in a fish model as revealed by viral DNA microarrays

et al. 2007

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Red seabream iridovirus (RSIV) disease is a serious disease of many marine fish species in Japan and elsewhere. For a better understanding of the molecular pathogenic mechanism, we examined the transcriptional profile of RSIV in infected fish using a DNA microarray. Expression of RSIV open reading frames (ORFs) was first detected at about 5 days post-infection (d.p.i.), and accounted for about 45% of total ORFs. Almost all the ORFs (97-99%) were expressed at their maximum levels during 7-9 d.p.i. The expression levels and the number of expressed ORFs started to decrease at 10 d.p.i. These results suggest that pathogenesis of RSIV infection began at around day 5, and continued with high levels of viral multiplication until viral clearance by host antiviral defenses starting from around 10 d.p.i. A comparison of viral gene expressions in the spleen and kidney over the course of the infection suggests that RSIV preferentially targets the spleen. The spleen may thus be the most susceptible organ for diagnosis of iridoviral disease.

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Section: Discussionsupporting

confidence: 79%

Section: Discussionmentioning

confidence: 99%

Transcriptional profile of red seabream iridovirus in a fish model as revealed by viral DNA microarrays

et al. 2007

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“…MCP and PolB were proposed to be good candidates for studying evolution and diversity in NCLDV, either because they contain conserved domains surrounding divergent regions (13,21) or because a correlation between genetic distances of partial fragments and hybridization of total genomic DNA was found (8). However, no primer sets were specifically tailored to describe Prasinovirus diversity in the environment using amplicon sequencing with high-throughput technologies, even though this approach allows diversity at the community level to be described and avoids the biases of culturedependent methods.…”

Section: Discussionmentioning

confidence: 99%

Unveiling of the Diversity of Prasinoviruses (Phycodnaviridae) in Marine Samples by Using High-Throughput Sequencing Analyses of PCR-Amplified DNA Polymerase and Major Capsid Protein Genes

Clérissi

Grimsley

Ogata

et al. 2014

Appl Environ Microbiol

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e Viruses strongly influence the ecology and evolution of their eukaryotic hosts in the marine environment, but little is known about their diversity and distribution. Prasinoviruses infect an abundant and widespread class of phytoplankton, the Mamiellophyceae, and thereby exert a specific and important role in microbial ecosystems. However, molecular tools to specifically identify this viral genus in environmental samples are still lacking. We developed two primer sets, designed for use with polymerase chain reactions and 454 pyrosequencing technologies, to target two conserved genes, encoding the DNA polymerase (PolB gene) and the major capsid protein (MCP gene). While only one copy of the PolB gene is present in Prasinovirus genomes, there are at least seven paralogs for MCP, the copy we named number 6 being shared with other eukaryotic alga-infecting viruses. Primer sets for PolB and MCP6 were thus designed and tested on 6 samples from the Tara Oceans project. The results suggest that the MCP6 amplicons show greater richness but that PolB gave a wider coverage of Prasinovirus diversity. As a consequence, we recommend use of the PolB primer set, which will certainly reveal exciting new insights about the diversity and distribution of prasinoviruses at the community scale.

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“…Comparing the LCDV-1 and LCDV-C genomes, the highest nucleotide sequence identity was observed in the major capsid protein (MCP) gene (78.9%), which encodes a single 50 kDa polypeptide [9][10][11]. The mcp gene is one of the most important molecular markers for analyzing the genetic relationships among iridoviruses because its nucleotide sequence is relatively conserved within the Iridoviridae family [12,13]. Recently, several LCDV genotypes were reported, based on the mcp gene and the pathogenicity of the virus [3,[14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Analysis of the genetic diversity of the lymphocystis virus and its evolutionary relationship with its hosts

Yan

Jian

et al. 2011

Virus Genes

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Lymphocystis disease virus (LCDV) is the causative agent of lymphocystis disease. In this study, the mcp gene of LCDV and the cyt b gene of the host fish were selected as molecular markers, and the phylogenetic relationships between LCDV and its host were analyzed. The 25 LCDV isolates examined in this study were attributed to seven LCDV genotypes: genotype I (LCDV-1), genotype II (LCDV-cn, etc.), genotype III (LCDV-rf), genotype IV (LCDV-rc and LCDV-sb), genotype V (LCDV-cb), genotype VI (LCDV-tl), and genotype VII (LCDV-sa). Genotype VII is a new genotype. LCDV1 was found to have differentiated first, followed by LCDV-rf; then LCDV-tl; LCDV-cb; and then LCDV-sa; and by LCDV-rc and LCDV-sb; and finally by LCDV-cn, LCDV-C, and LCDV-jf. From the host evolutionary perspective, Rachycentron canadum was found to have differentiated first, followed by Trichogaster leeri, Chanda baculis, and Sebastes schlegeli, Lateolabrax sp., Sparus aurata, Platichthys flesus, and Paralichthys olivaceus. Comparison of the phylogenies of the host fish species and LCDVs revealed no significant evidence of cospeciation between LCDVs and their host fish. In-depth studies of the genetic variation in LCDVs can enhance our understanding of the mechanism of LCDV infection, which may provide important insights into the prevention and treatment of lymphocystis disease.

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Cited by 155 publications

References 29 publications

Transcriptional profile of red seabream iridovirus in a fish model as revealed by viral DNA microarrays

Transcriptional profile of red seabream iridovirus in a fish model as revealed by viral DNA microarrays

Unveiling of the Diversity of Prasinoviruses (Phycodnaviridae) in Marine Samples by Using High-Throughput Sequencing Analyses of PCR-Amplified DNA Polymerase and Major Capsid Protein Genes

Analysis of the genetic diversity of the lymphocystis virus and its evolutionary relationship with its hosts

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