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Mohapatra, U.; McCabe, Matthew S.; Power, J. B.; Schepers, Frank; Arend, A. van der; Davey, M. R.

doi:10.1023/a:1008891216134

Cited by 29 publications

(5 citation statements)

References 32 publications

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“…The selection system based on the marker gene bar (Fig. 1), conferring resistance to strongly acting glufosinate, probably reduced the transformation rate, but, on the other hand, it guarantees high probability (90%, see Results section) of regeneration of authentic transformants in comparison to kanamycin (Mohapatra et al 1999). The obtained 25 plant lines were analyzed to select 12 lines characterized by a normal phenotype, stable inheritance of the S-HBs transgene (Fig.…”

Section: Discussionmentioning

confidence: 99%

Low-dose oral immunization with lyophilized tissue of herbicide-resistant lettuce expressing hepatitis B surface antigen for prototype plant-derived vaccine tablet formulation

et al. 2010

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Efficient immunization against hepatitis B virus (HBV) and other pathogens with plant-based oral vaccines requires appropriate plant expressors and the optimization of vaccine compositions and administration protocols. Previous immunization studies were mainly based on a combination of the injection of a small surface antigen of HBV (S-HBsAg) and the feeding with raw tissue containing the antigen, supplemented with an adjuvant, and coming from plants conferring resistance to kanamycin. The objective of this study was to develop a prototype oral vaccine formula suitable for human immunization. Herbicide-resistant lettuce was engineered, stably expressing through progeny generation micrograms of S-HBsAg per g of fresh weight and formed into virus-like particles (VLPs). Lyophilized tissue containing a relatively low, 100-ng VLP-assembled antigen dose, administered only orally to mice with a long, 60-day interval between prime and boost immunizations and without exogenous adjuvant, elicited mucosal and systemic humoral anti-HBs responses at the nominally protective level. Lyophilized tissue was converted into tablets, which preserved S-HBsAg content for at least one year of room temperature storage. The results of the study provide indications on immunization methodology using a durable, efficacious, and convenient plant-derived prototype oral vaccine against hepatitis B.

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Section: Discussionmentioning

confidence: 99%

Low-dose oral immunization with lyophilized tissue of herbicide-resistant lettuce expressing hepatitis B surface antigen for prototype plant-derived vaccine tablet formulation

et al. 2010

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“…Lettuce is a suitable candidate for the improvement of agricultural traits, containing the transfer of nutrients and bioactive compounds valuable to human health. So, providing a good managing strategy for lettuce-growing culture including microbial resistance [1], environmental stress tolerance [2,3], 2 of 18 yield enhancement [4], and increased active biomolecules [5] is crucially important in the agriculture sector. Today, plant productivity is an ever-growing issue that attracts researchers worldwide to apply eco-friendly innovation techniques for crop development.…”

Section: Introductionmentioning

confidence: 99%

Impact of the Static Magnetic Field on Growth, Pigments, Osmolytes, Nitric Oxide, Hydrogen Sulfide, Phenylalanine Ammonia-Lyase Activity, Antioxidant Defense System, and Yield in Lettuce

Latef

Hassanpour

Rezayian

et al. 2020

Biology

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Magnetic fields are an unavoidable physical factor affecting living organisms. Lettuce seeds (Lactuca sativa var. cabitat L.) were subjected to various intensities of the static magnetic field (SMF) viz., MF0 (control), SMF1 (0.44 Tesla (T), SMF2 (0.77 T), and SMF3 (1 T) for three exposure times (1, 2, and 3 h). SMF-treated seedlings showed induction in growth parameters and metabolism comparing to control. All photosynthetic pigments were induced markedly under SMF, especially chlorophyll a. SMF at different intensities boosted osmolytes, non-enzymatic antioxidants, and the phenylalanine ammonia-lyase activity over non-magnetized seedlings. Oxidative damage criteria viz., hydrogen peroxide, superoxide radical, and lipid peroxidation, as well as polyphenol oxidase activity, were kept at low values under SMF-treated seeds relative to control, especially SMF2. Electron donors to antioxidant enzymes including nitrate reductase, nitric oxide, and hydrogen sulfide induced via SMF exposure and consequently the activities of superoxide dismutase, glutathione-S-transferases, catalase, and peroxidases family enzymes were also stimulated under SMF, whatever the intensity or the exposure period applied. All these regulations reflected on the enhancement of lettuce yield production which reached 50% over the control at SMF3. Our findings offered that SMF-seed priming is an innovative and low-cost strategy that can improve the growth, bioactive constituents, and yield of lettuce.

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“…Lettuce is therefore a good candidate for agronomic trait improvement, including the delivery of nutrients and bioactive compounds beneficial to human health. Following this line of reasoning, lettuce varieties with traits such as herbicide resistance [2], virus resistance [3], environmental stress resistance [4, 5], yield enhancement [6], accumulation of pharmaceutical proteins [7, 8], increased leaf calcium content [9], and increased total polyphenol content [10, 11], have been developed.…”

Section: Introductionmentioning

confidence: 99%

A rapid and efficient in vitro regeneration system for lettuce (Lactuca sativa L.)

2017

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BackgroundSuccessful biotechnological improvement of crop plants requires a reliable and efficient in vitro regeneration system. Lettuce (Lactuca sativa L.), one the most important vegetable crops worldwide, is strongly genotype-dependent in terms of regeneration capacity, limiting the potential for biotechnological improvement of cultivars which show recalcitrance under currently available protocols. The effect of different nutrient sources, plant hormone combinations and activated charcoal supplementation on shoot induction efficiency was evaluated on the cultivar ‘RSL NFR’, which had previously shown poor regeneration efficiency.ResultsMultiple shoot organogenesis from cotyledon explants was recorded at the highest frequency and speed on Murashige and Skoog regeneration medium supplemented with 200 mg/l of activated charcoal, 3% sucrose, 10 mg/l benzylaminopurine and 0.5 mg/l naphthaleneacetic acid, which induced shoots through direct regeneration in 90.8 ± 7.9% of explants. High shoot induction efficiency was also observed, albeit not quantified, when using this medium on some other cultivars.ConclusionsThis activated charcoal-containing regeneration medium might offer a rapid and efficient option for direct shoot induction in some lettuce genotypes that do not respond well to common lettuce regeneration protocols. This is also the first report of the effect of activated charcoal in lettuce tissue culture.Electronic supplementary materialThe online version of this article (doi:10.1186/s13007-017-0208-0) contains supplementary material, which is available to authorized users.

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Cited by 29 publications

References 32 publications

Low-dose oral immunization with lyophilized tissue of herbicide-resistant lettuce expressing hepatitis B surface antigen for prototype plant-derived vaccine tablet formulation

Low-dose oral immunization with lyophilized tissue of herbicide-resistant lettuce expressing hepatitis B surface antigen for prototype plant-derived vaccine tablet formulation

Impact of the Static Magnetic Field on Growth, Pigments, Osmolytes, Nitric Oxide, Hydrogen Sulfide, Phenylalanine Ammonia-Lyase Activity, Antioxidant Defense System, and Yield in Lettuce

A rapid and efficient in vitro regeneration system for lettuce (Lactuca sativa L.)

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