50Hz Extremely Low Frequency Electromagnetic Fields Enhance Protein Carbonyl Groups Content in Cancer Cells: Effects on Proteasomal Systems

Eleuteri, Anna Maria; Amici, Manila; Bonfili, Laura; Cecarini, Valentina; Cuccioloni, Massimiliano; Grimaldi, Settimio; Giuliani, Livio; Angeletti, Mauro; Fioretti, Evandro

doi:10.1155/2009/834239

Cited by 25 publications

(21 citation statements)

References 64 publications

(68 reference statements)

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“…It is possible to argue that the decrease of cell proliferation following ELF-EMF exposure could be related to actin filaments rearrangement, since actin polymerization is one of the mechanisms regulating formation of contractile ring in cytokinesis (Lacy-Hulbert, 1998;Blank, 2007). There are quite a few studies analyzing the effects of ELF-EMF on different cell lines, which showed that ELF-EMF treatment in the early stages accelerated cellular proliferation in a time-dependent manner (Wolf, 2005;Manni, 2002;Eleuteri, 2009). Cells that were exposed to 100 Hz showed different behavior and aberrant bundles of F-actin appeared.…”

Section: Discussionmentioning

confidence: 99%

Effects of extremely low-frequency pulsed electromagnetic fields on morphological and biochemical properties of human breast carcinoma cells (T47D)

Sadeghipour

Ahmadian

Bolouri

et al. 2012

Electromagnetic Biology and Medicine

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This study was carried out to investigate the effects of 100 and 217 Hz extremely low-frequency pulsed electromagnetic fields (ELF-PEMF) on cell proliferation, actin reorganization, and ROS generation in a human breast carcinoma cells (T47D). Cells were exposed for 24-72 h, at 100 and 217 Hz, 0.1 mT. The treatment induced a time dependent decrease in cell growth after 72 h and revealed an increase in fluorescence intensity in cytoplasm and actin aggregations around the nucleus as detected by fluorescence microscopy. The amount of actin in T47D cells increased after 48 h exposure to 100 Hz and 24 h to 217 Hz while no changes in nuclear morphology were detected. Exposing the cells to 217 Hz for 72 h caused a dramatically increase of intracellular ROS generation while with exposure to 100 Hz it remained nearly unchanged. These results suggest that exposure to ELF-PEMF (100, 217 Hz, 0.1 mT) are able inducing an increase of actin level, its migration toward nucleus but despite of these changes and dramatically increase in ROS generation the symptoms of apoptosis were not observed. Our results support the hypothesis that cell response to EMF may only be observed at certain window effects; such as frequency and intensity of EMF parameters.

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Section: Discussionmentioning

confidence: 99%

Effects of extremely low-frequency pulsed electromagnetic fields on morphological and biochemical properties of human breast carcinoma cells (T47D)

Sadeghipour

Ahmadian

Bolouri

et al. 2012

Electromagnetic Biology and Medicine

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“…However, the proliferation response has been investigated by a scarce number of studies, most of which applied magnetic flux densities above the values of 500 µT and 100 µT proposed by ICNIRP [12] for protection of the workers and the general public, respectively, against exposure to 50-Hz MF. As a whole, the results of these studies have been considered inconsistent and contradictory, since they have reported proliferative effects [13,14], antiproliferative responses [15][16][17] or no effect [18]. It has been proposed that such inconsistency could be due to the fact that the MF-induced biological responses are influenced by a number of biological factors, like the genetic characteristics or differentiation status of the cells, or physical factors, like some field parameters or the chronological pattern of exposure [8,[19][20][21][22][23][24].…”

Section: Introductionmentioning

confidence: 99%

The Proliferative Response of NB69 Human Neuroblastoma Cells to a 50 Hz Magnetic Field is mediated by ERK1/2 Signaling

Martínez

Úbeda

Cid³

et al. 2012

Cell Physiol Biochem

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A number of studies have reported that extremely low frequency magnetic fields (ELF-MF) can modulate proliferative processes in vitro; however, the transduction mechanisms implicated in such phenomena remain to be identified. The present study was aimed to determine whether a 50 Hz, 100 µT MF can induce cell proliferation in the human neuroblastoma line NB69, and whether the signaling pathway MAPK-ERK1/2 (Mitogen-Activated Protein Kinase - Extracellular-Signal-Regulated Kinase 1 and 2) is involved in that proliferative response. The cultures were exposed intermittently or continuously to the MF for a 63-hour duration. The continuous treatment did not induce significant changes in cell proliferation. In contrast, intermittent exposure caused statistically significant increase in the percent of cells in phase S of the cell cycle, followed by a significant increase in cell number. The intermittent treatment also induced an early, transient and repetitive activation of ERK1/2 that could be involved in the proliferative effects. In fact, both the proliferative response and the repeated activation of ERK1/2 were blocked by PD98059, the specific inhibitor of MEK (ERK kinases 1 and 2). Taken together, the described results indicate that a 50 Hz, 100 µT MF can stimulate proliferation in NB69 cells by triggering MAPK-ERK1/ 2 signaling at each of the “On” periods of an intermittent exposure.

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“…However, to date there is no general agreement on the specific primary biological targets of power frequency MFs, on the biophysical and chemical basis of the MF interactions, nor to which extent the reported in vitro bioeffects may be indicative of a human susceptibility to these fields. Concerning potential targets related to cancer risk, ELF MFs have been shown to alter gene and protein expression (16–18) as well as intracellular calcium concentration and apoptosis rates (19–23) and to induce oxidative stress (24–26) and DNA damage (27–31). The interest in the potential adverse effects of power frequency MFs has also been focused on the possibility that these fields may influence tumor promotion by increasing the rate of cell proliferation and/or modifying the activity of molecules implicated in their regulation.…”

Section: Introductionmentioning

confidence: 99%

Retinoic acid inhibits the cytoproliferative response to weak 50-Hz magnetic fields in neuroblastoma cells

et al. 2012

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We previously reported that intermittent exposure to a 50-Hz magnetic field (MF) at 100 μT stimulates cell proliferation in the human neuroblastoma cell line NB69. The present study aimed to investigate whether the magnetic field-induced growth promotion also occurs at a lower magnetic flux density of 10 μT. To this purpose, NB69 cells were subjected for 42 h to intermittent exposure, 3 h on/3 h off, to a 50-Hz MF at a 10 or 100 μT magnetic flux density. The field exposure took place either in the presence or in the absence of the antiproliferative agent retinoic acid. At the end of the treatment and/or incubation period, the cell growth was estimated by hemocytometric counting and spectrophotometric analysis of total protein and DNA contents. Potential changes in DNA synthesis were also assessed through proliferating cell nuclear antigen (PCNA) immunolabeling. The results confirmed previously reported data that a 42-h exposure to a 50-Hz sine wave MF at 100 μT promotes cell growth in the NB69 cell line, and showed that 10 μT induces a similar proliferative response. This effect, which was significantly associated and linearly correlated with PCNA expression, was abolished by the presence of retinoic acid in the culture medium.

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50Hz Extremely Low Frequency Electromagnetic Fields Enhance Protein Carbonyl Groups Content in Cancer Cells: Effects on Proteasomal Systems

Cited by 25 publications

References 64 publications

Effects of extremely low-frequency pulsed electromagnetic fields on morphological and biochemical properties of human breast carcinoma cells (T47D)

Effects of extremely low-frequency pulsed electromagnetic fields on morphological and biochemical properties of human breast carcinoma cells (T47D)

The Proliferative Response of NB69 Human Neuroblastoma Cells to a 50 Hz Magnetic Field is mediated by ERK1/2 Signaling

Retinoic acid inhibits the cytoproliferative response to weak 50-Hz magnetic fields in neuroblastoma cells

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