50 years of comparative biochemistry: The legacy of Peter Hochachka

Buck, Leslie T.; Burness, Gary; Campbell, Kevin L.; Darveau, Charles-A.; Driedzic, William R.; Guderley, Helga; McClelland, Grant B.; Moon, Thomas W.; Moyes, Christopher D.; Schulte, Patricia M.

doi:10.1016/j.cbpb.2018.02.002

Cited by 3 publications

(1 citation statement)

References 132 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…CD8+ T cell infiltration degree can be used as an indicator of tumor prognosis [ 28 ]. The immune infiltration of CD8+T cells is negatively regulated by the tumor microenvironment, leading to tumor cells evading immune surveillance and thus promoting tumor progression [ 9 ].…”

Section: Discussionmentioning

confidence: 99%

LINC00887 Fosters Development of Clear Cell Renal Cell Carcinoma via Inhibiting CD8+ T Cell Immune Infiltration

Lin

Zhang

et al. 2022

Computational and Mathematical Methods in Medicine

View full text Add to dashboard Cite

Background. lncRNAs affect adaptive and innate immunity of cancer via mediating functional states of immune cells, genes, and pathways. Nonetheless, little is known about the molecular mechanism of lncRNA-mediated CD8+ T cell immune infiltration in progression of clear cell renal cell carcinoma (ccRCC). We designed this work to investigate the role of LINC00887 in regulating CD8+ T cell immune infiltration in ccRCC. Methods. Correlation between LINC00887 and immune factors and the expression level of LINC00887 in ccRCC were analyzed by bioinformatics methods (TCGA-KIRC database, “edgeR” package, “clusterProfiler” package, and “CIBERSORT” package). LINC00887 expression in ccRCC was examined via RT-qPCR. The cytokilling capacity of CD8+ T cells was evaluated by the lactate dehydrogenase assay. The apoptotic ability of CD8+ T cells was measured by flow cytometry. The chemotactic ability of CD8+ T cells was revealed by chemotaxis assay. CXCR3, CXCL9, and CXCL10 levels were assessed by RT-qPCR. Results. As suggested by bioinformatics analysis, LINC00887 was markedly upregulated in ccRCC patients and associated with expression of immune-suppression molecule, thereby abating the immune infiltration level of CD8+ cells in tumor tissue. As revealed by cellular assay, LINC00887 was upregulated in ccRCC cells, and knockdown of LINC00887 resulted in a decreased PD-L1 expression, increased CD8+ T cell toxicity, decreased apoptotic levels, and enhanced chemotaxis. Moreover, we found that LINC00887 exhibited inhibitory effect on immune infiltration of CD8+ cells in clinical tissues. Conclusions. The results of this study suggested that LINC00887 promoted ccRCC progression by inhibiting immune infiltration of CD8+ T cells, providing new insights into pathogenesis of ccRCC and suggesting LINC00887 being a promising immunotherapy target for ccRCC.

show abstract

Section: Discussionmentioning

confidence: 99%

LINC00887 Fosters Development of Clear Cell Renal Cell Carcinoma via Inhibiting CD8+ T Cell Immune Infiltration

Lin

Zhang

et al. 2022

Computational and Mathematical Methods in Medicine

View full text Add to dashboard Cite

show abstract

Application of Omics Approaches to Improve Bioinoculant Performance

Gangola,

Joshi,

Goswami

et al. 2024

Interdisciplinary Biotechnological Advances

View full text Add to dashboard Cite

Bioinoculant capability enhancement through metabolomics and systems biology approaches

Chaudhary

Shukla

2018

Briefings in Functional Genomics

View full text Add to dashboard Cite

Bioinoculants are eco-friendly microorganisms, and their products are utilized for improving the potential of soil and fulfill the nutrients requirement for the host plant. The agricultural yield has increased due to the use of bioinoculants over chemical-based fertilizers, and thus it generates interest in understanding the innovation process by various methods. By gene-editing tool, the desired gene product can be changed for engineered microbial inoculants. We have also described various modern biotechnological tools like constraint-based modeling, OptKnock, flux balance analysis and modeling of the biological network for enhancing the bioinoculant capability. These fluxes give the fascinating perception of the metabolic network in the absence of comprehensive kinetic information. These tools also help in the stimulation of the metabolic networks by incorporation of enzyme-encoding genes. The present review explains the use of systems biology and gene-editing tools for improving the capability of bioinoculants. Moreover, this review also emphasizes on the challenges and future perspective of systems biology and its multidisciplinary facets.

show abstract

50 years of comparative biochemistry: The legacy of Peter Hochachka

Cited by 3 publications

References 132 publications

LINC00887 Fosters Development of Clear Cell Renal Cell Carcinoma via Inhibiting CD8+ T Cell Immune Infiltration

LINC00887 Fosters Development of Clear Cell Renal Cell Carcinoma via Inhibiting CD8+ T Cell Immune Infiltration

Application of Omics Approaches to Improve Bioinoculant Performance

Bioinoculant capability enhancement through metabolomics and systems biology approaches

Contact Info

Product

Resources

About

50 years of comparative biochemistry: The legacy of Peter Hochachka

Cited by 3 publications

References 132 publications

LINC00887 Fosters Development of Clear Cell Renal Cell Carcinoma via Inhibiting CD8+ T Cell Immune Infiltration

LINC00887 Fosters Development of Clear Cell Renal Cell Carcinoma via Inhibiting CD8+ T Cell Immune Infiltration

Application of Omics Approaches to Improve Bioinoculant Performance

Bioinoculant capability enhancement through metabolomics and systems biology approaches

Contact Info

Product

Resources

About

50 years of comparative biochemistry: The legacy of Peter Hochachka