5'-Ribonucleotides production using 5'-phosphodiesterase from spent malt roots

Abstract

5'-ribonucleotides are high value-added molecules widely used in the food and pharmaceutical industries because of their bioactive properties. The present work aims to produce a composition of 5’-ribonucleotides using spent brewer’s yeast as cheap source of RNA, and barley malt rootlets as cheap source of 5'-phosphodiesterase (5'-PDE). This is a very promising and innovative strategy because both spent yeast and malt rootles are residues of the brewing process and are closely linked in a cycle that until now is not yet commercially exploited due to lack of studies. Our results showed that extraction of 5’-PDE was mainly influenced by the fineness of the rootlets and amount of extraction solvent (water). The main molecules formed during RNA hydrolysis were 5’-ribonucleotides, which represented 85.86% of the total hydrolyzed molecules. Finally, the results of the proposed approach can generate a new perspective for the brewing industry regarding the management of its wastes, generating from them products of high added value and with a wide range of applications.

Recovery of High-Value Compounds from Yarrowia lipolytica IMUFRJ 50682 Using Autolysis and Acid Hydrolysis

“…Finally, the suspension was centrifuged (2.057 g, 5 min, 20 • C), and the supernatant was reserved for ultrafiltration separation. The precipitate was dried in an oven (60 • C, 18 h), its granulometry was standardized to 0.5 mm, and the dry mass was characterized as a food ingredient [34,38].…”

“…For example, autolysis is a feasible first step due to the mild temperature, pH, time conditions, and great penetration power [34]. This method showed promising results when combined with acid shock and pulsed electric fields to extract RNA and mannoprotein [22,38].…”