5. Response of the immature rat to androgen and estrogen following treatment on day one of life with estrogen, testosterone or an estrogen antagonist

Lerner, LeonardJ.; Vitale, A

doi:10.1016/0022-4731(75)90222-8

Cited by 1 publication

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“…PM, perimetrial surface;epi, epithelium. al., 1977;McLachlan et al, 1980;Gilloteaux et al, 1982;Maier et al, 1985;Forsberg et al, 1985;Ostrander et al, 1985;Iguchi and Takasugi, 1987) and a decreased responsiveness to the growth promoting effects of estrogen in adulthood (Mori, 1967(Mori, , 1975Takasugi et al, 1970;Lerner et al, 1977;Gellert et al, 1977;Aihara et al, 1980;Maier et al, 1985;Ostrander et al, 1985;Iguchi and Takasugi, 1987). Uterine hy- poplasia also observed in this study is likely to be the consequence of disturbances in uterine mesenchymal growth and differentiation, since the derivatives of uterine mesenchyme (endometrial stroma and myometrium) normally give rise to 90-95% of the uterine mass (Martin et al, 1973;McCormack and Glasser, 1980) and define the overall size and shape of the uterus (Konishi et al, 1984).…”

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confidence: 97%

Histologic, morphometric, and immunocytochemical analysis of myometrial development in rats and mice: II. Effects of DES on development

Brody

Cunha

1989

Am. J. Anat.

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The effects of diethylstilbestrol (DES) treatment on myometrial development from the prenatal to adult period were examined in rats and mice by histologic and immunocytochemical methods using anti-actin, -vimentin, and -laminin to assess cytodifferentiation of smooth muscle and fibroblastic cells, and by morphometric procedures to assess quantitatively the effect of DES on the expression of cellular orientation in the emerging inner circular myometrial layer. Neonatal rats and mice were treated with DES from day 0 (day of birth) to day 2 with dosages known to perturb myometrial development. Neonatal treatment with DES increased the degree of circular orientation within the uterine mesenchyme, an effect detectable following as little as 24 hr of DES treatment. This effect on spatial organization of the mesenchyme was followed by an increase in the thickness of the actin-positive middle layer (prospective circular myometrium) of uterine mesenchyme during days 3-15; from day 15 onward, however, the circular myometrial layer began to fragment into irregular bundles of smooth muscle, and the longitudinal myometrial layer became thinner and more irregularly organized than controls. Vimentin localization in rats treated with DES neonatally was more intense than in controls within the circularly orientated uterine mesenchyme at 5 days. By 60 days the circular and longitudinal myometrial layers of DES-treated animals showed strands and bundles of vimentin-positive cells, which were not present in controls. Both rats and mice show comparable effects of DES treatment.

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