5′ processing of Saccharomyces cerevisiae mitochondrial tRNAs requires expression of multiple genes

Guedes‐Monteiro, Raquel Fonseca; Franco, Leticia Veloso Ribeiro; Moda, Bruno S.; Tzagoloff, Alexander; Barros, Mário H.

doi:10.1016/j.bbamcr.2019.02.002

Cited by 7 publications

(9 citation statements)

References 50 publications

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“…Primers were designed based on ClustalW alignments of the studied mitoribosome mtLSU components with bacterial counterparts. After PCR amplification, DNA fragments were cloned into YIp349 (McStay et al, 2013) or into YCp22NATP9 (Guedes‐Monteiro et al, 2019) in which the gene was cloned in‐frame to Neurospora crassa nuclear ATP9 mitochondrial targeting sequence (MTS) (Table S2).…”

Section: Methodsmentioning

confidence: 99%

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Functional analyses of mitoribosome 54S subunit devoid of mitochondria‐specific protein sequences

Santos

Zhao

Jorge

et al. 2021

Yeast

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In Saccharomyces cerevisiae, mitoribosomes are composed of a 54S large subunit (mtLSU) and a 37S small subunit (mtSSU). The two subunits altogether contain 73 mitoribosome proteins (MRPs) and two ribosomal RNAs (rRNAs). Although mitoribosomes preserve some similarities with their bacterial counterparts, they have significantly diverged by acquiring new proteins, protein extensions, and new RNA segments, adapting the mitoribosome to the synthesis of highly hydrophobic membrane proteins. In this study, we investigated the functional relevance of mitochondria‐specific protein extensions at the C‐terminus (C) or N‐terminus (N) present in 19 proteins of the mtLSU. The studied mitochondria‐specific extensions consist of long tails and loops extending from globular domains that mainly interact with mitochondria‐specific proteins and 21S rRNA moieties extensions. The expression of variants devoid of extensions in uL4 (C), uL5 (N), uL13 (N), uL13 (C), uL16 (C), bL17 (N), bL17 (C), bL21 (24), uL22 (N), uL23 (N), uL23 (C), uL24 (C), bL27 (C), bL28 (N), bL28 (C), uL29 (N), uL29 (C), uL30 (C), bL31 (C), and bL32 (C) did not rescue the mitochondrial protein synthesis capacities and respiratory growth of the respective null mutants. On the contrary, the truncated form of the mitoribosome exit tunnel protein uL24 (N) yields a partially functional mitoribosome. Also, the removal of mitochondria‐specific sequences from uL1 (N), uL3 (N), uL16 (N), bL9 (N), bL19 (C), uL29 (C), and bL31 (N) did not affect the mitoribosome function and respiratory growth. The collection of mutants described here provides new means to study and evaluate defective assembly modules in the mitoribosome biogenesis process.

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Section: Methodsmentioning

confidence: 99%

“…After PCR amplification, DNA fragments were cloned into YIp349 (McStay et al, 2013) or into YCp22NATP9 (Guedes-Monteiro et al, 2019) in which the gene was cloned in-frame to Neurospora crassa nuclear ATP9 mitochondrial targeting sequence (MTS) (Table S2).…”

Section: Plasmid Constructionsmentioning

confidence: 99%

Functional analyses of mitoribosome 54S subunit devoid of mitochondria‐specific protein sequences

Santos

Zhao

Jorge

et al. 2021

Yeast

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“…D , schematic representation of the W303-1B yeast strain mitochondrial primary RNA transcripts. COB and COX1 exons are represented with capital letters and the introns with lowercases letters , double arrows positioned the probes employed in the Northern blots as follows: COX2 entire coding sequence, COB a 23 nucleotide primer positioned just after the first ATG ( Table S2 , ( 95 )); COX1 exon a4 entire sequence. Scale and graphics were based on a previous review ( 77 ).…”

Section: Resultsmentioning

confidence: 99%

Overexpression of MRX9 impairs processing of RNAs encoding mitochondrial oxidative phosphorylation factors COB and COX1 in yeast

Chagas

Soares

Franco

et al. 2022

Journal of Biological Chemistry

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“…51,52 In S. cerevisiae, four additional accessory factors, Mta1p, Mta2p, Gep5p, and Pet130p, that are required for the efficient processing of tRNA precursors by RNase P were recently identified. 53 Even though RNase P activity is universally present in eukaryotic mitochondria, its composition is not conserved and multiple lineages, including budding yeasts such as C. albicans lack the mitochondrially encoded RNA subunit. 5,10 The mitochondrial tRNase Z activity in S. cerevisiae is assured by the Trz1 protein which also functions in nuclear tRNA processing, and is thus essential.…”

Section: Processing and Maturation Of Mitochondrial Rnasmentioning

confidence: 99%

RNA processing and degradation mechanisms shaping the mitochondrial transcriptome of budding yeasts

Golik

2023

IUBMB Life

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Yeast mitochondrial genes are expressed as polycistronic transcription units that contain RNAs from different classes and show great evolutionary variability. The promoters are simple, and transcriptional control is rudimentary. Posttranscriptional mechanisms involving RNA maturation, stability, and degradation are thus the main force shaping the transcriptome and determining the expression levels of individual genes. Primary transcripts are fragmented by tRNA excision by RNase P and tRNase Z, additional processing events occur at the dodecamer site at the 3′ end of protein‐coding sequences. groups I and II introns are excised in a self‐splicing reaction that is supported by protein splicing factors encoded by the nuclear genes, or by the introns themselves. The 3′‐to‐5′ exoribonucleolytic complex called mtEXO is the main RNA degradation activity involved in RNA turnover and processing, supported by an auxiliary 5′‐to‐3′ exoribonuclease Pet127p. tRNAs and, to a lesser extent, rRNAs undergo several different base modifications. This complex gene expression system relies on the coordinated action of mitochondrial and nuclear genes and undergoes rapid evolution, contributing to speciation events. Moving beyond the classical model yeast Saccharomyces cerevisiae to other budding yeasts should provide important insights into the coevolution of both genomes that constitute the eukaryotic genetic system.

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5′ processing of Saccharomyces cerevisiae mitochondrial tRNAs requires expression of multiple genes

Cited by 7 publications

References 50 publications

Functional analyses of mitoribosome 54S subunit devoid of mitochondria‐specific protein sequences

Functional analyses of mitoribosome 54S subunit devoid of mitochondria‐specific protein sequences

Overexpression of MRX9 impairs processing of RNAs encoding mitochondrial oxidative phosphorylation factors COB and COX1 in yeast

RNA processing and degradation mechanisms shaping the mitochondrial transcriptome of budding yeasts

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