5[prime] Proximal Regions of Arabidopsis Nitrate Reductase Genes Direct Nitrate-Induced Transcription in Transgenic Tobacco

Lin, Yuwei; Hwang, Chin-Feng; Brown, Jennifer B.; Cheng, Chi‐Lien

doi:10.1104/pp.106.2.477

Cited by 58 publications

(51 citation statements)

References 32 publications

(38 reference statements)

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“…The NIT-2 motif has been widely found in genes for the enzymes involved in nitrogen metabolism (Marzluf, 1993). In higher plants these motifs have been found in the 5' flanking regions of the genes for NR (Lin et al, 1994) and NiR (Tanaka et al, 1994) in Arabidopsis thaliana, and for FNR in Oryza sativa (Aoki et al, 1995), as listed in Table I.…”

Section: Discussionmentioning

confidence: 99%

A Nitrate-Inducible Ferredoxin in Maize Roots (Genomic Organization and Differential Expression of Two Nonphotosynthetic Ferredoxin Isoproteins)

et al. 1997

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We have identified and characterized a nitrate-inducible ferredoxin (Fd) i n maize (Zea mays L.) roots by structural analysis of the purified protein and by cloning of i t s cDNA and gene. In maize Fd isoproteins are encoded by a small multigene family, and the nitrate-inducible Fd was identified as a novel isoprotein, designated Fd VI, which differed from any Fd I to Fd V identified to date. In the roots of seedlings cultured without nitrate, Fd VI was undetectable.However, during the induction of the capacity for nitrate assimilation, the amount of Fd VI increased markedly within 24 h. Concurrentiy, the levei of transcript for Fd VI increased, but more quickly, reaching a maximal leve1 within 2 h with kinetics similar to those of nitrite reductase and Fd-NADP+ reductase. Fd 111 was constitutively expressed in roots, and no such changes at the protein and mRNA levels were observed during the nitrate induction. I n the 5' flanking region of the gene for Fd VI only, we identified NIT-2 motifs, which are widely found in genes for enzymes related to nitrogen metabolism. These data indicate that Fd V I i s co-induced with the previously characterized enzymes involved i n nitrate assimilation, and they suggest that the novel Fd isoprotein, distinct from the constitutively expressed Fd, might play an important role as an electron carrier from NADPH to nitrite reductase and other Fd-dependent enzymes in root plastids.

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Section: Discussionmentioning

confidence: 99%

A Nitrate-Inducible Ferredoxin in Maize Roots (Genomic Organization and Differential Expression of Two Nonphotosynthetic Ferredoxin Isoproteins)

et al. 1997

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“…Deletion analysis of the 1.5-kb 5'-flanking regions of the Arabidopsis NIA1 and NIA2 genes showed that 238 and 188 bp, respectively, were important for nitrate induction of a reporter gene (CAT) in transgenic tobacco (Lin et al 1994). A 12 bp conserved sequence with a core consensus AG/CTCA, a "nitrate element", was necessary for nitrate dependent transcription (Hwang et al 1997).…”

Section: Nr Promoters and Light Regulationmentioning

confidence: 99%

Light Regulation of Nitrate uptake, Assimilation and Metabolism

Lillo

2004

Plant Ecophysiology

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“…Effects of NADK2 overexpression on GS/GOGAT pathway-related gene expression. A, Levels of GLN1;1, GLN1;3, GLN1;5, GS2, GLU1, GLT1, GDH1, and GDH2 transcripts in rosette leaves of 23- NIA and NIR expression (Pouteau et al, 1989;Lin et al, 1994;Scheible et al, 1997). In fact, NO 3 2 concentrations were approximately 10-fold higher in the nadk2 mutant (Fig.…”

Section: Nadk2 Overexpression Affects Both Gene Expression Associatedmentioning

confidence: 99%

Pleiotropic Modulation of Carbon and Nitrogen Metabolism in Arabidopsis Plants Overexpressing theNAD kinase2Gene

et al. 2009

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Nicotinamide nucleotides (NAD and NADP) are important cofactors in many metabolic processes in living organisms. In this study, we analyzed transgenic Arabidopsis (Arabidopsis thaliana) plants that overexpress NAD kinase2 (NADK2), an enzyme that catalyzes the synthesis of NADP from NAD in chloroplasts, to investigate the impacts of altering NADP level on plant metabolism. Metabolite profiling revealed that NADP(H) concentrations were proportional to NADK activity in NADK2 overexpressors and in the nadk2 mutant. Several metabolites associated with the Calvin cycle were also higher in the overexpressors, accompanied by an increase in overall Rubisco activity. Furthermore, enhanced NADP(H) production due to NADK2 overexpression increased nitrogen assimilation. Glutamine and glutamate concentrations, as well as some other amino acids, were higher in the overexpressors. These results indicate that overexpression of NADK2 either directly or indirectly stimulates carbon and nitrogen assimilation in Arabidopsis under restricted conditions. Importantly, since neither upregulation nor down-regulation of NADK2 activity affected the sum amount of NAD and NADP or the redox state, the absolute level of NADP and/or the NADP/NAD ratio likely plays a key role in regulating plant metabolism.

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5[prime] Proximal Regions of Arabidopsis Nitrate Reductase Genes Direct Nitrate-Induced Transcription in Transgenic Tobacco

Cited by 58 publications

References 32 publications

A Nitrate-Inducible Ferredoxin in Maize Roots (Genomic Organization and Differential Expression of Two Nonphotosynthetic Ferredoxin Isoproteins)

A Nitrate-Inducible Ferredoxin in Maize Roots (Genomic Organization and Differential Expression of Two Nonphotosynthetic Ferredoxin Isoproteins)

Light Regulation of Nitrate uptake, Assimilation and Metabolism

Pleiotropic Modulation of Carbon and Nitrogen Metabolism in Arabidopsis Plants Overexpressing theNAD kinase2Gene

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