5-Hydroxytryptamine metabolism in affective illness: the effect of tryptophan administration

Ashcroft, G. W.; Crawford, T. B. B.; Cundall, Richard; Davidson, David; Dobson, Janet; Dow, R. C.; Eccleston, D.; Loose, Robert W.; Pullar, Ian A.

doi:10.1017/s0033291700049618

Cited by 51 publications

(19 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Its presence in the cisternal rather than in the lumbar samples suggests that it reflects changes in the metabolism of 5HT with age, and not changes in the activity of the transport system which removes 5HIAA from the CSF. This view is supported by data from the study of Ashcroft et al 16 They found a quadratic relationship between lumbar CSF 5HIAA and age, very similar to that in fig 2, but only after the patients had received a load of tryptophan. If the quadratic relationship is due to a similar relationship between 5HT metabolism and age, then variation in one of two factors is presumably responsible, either the brain tryptophan content, or the activity of tryptophan hydroxylase.…”

Section: Age Differencesmentioning

confidence: 52%

Tryptophan, 5-hydroxyindoleacetic acid and indoleacetic acid in human cerebrospinal fluid: interrelationships and the influence of age, sex, epilepsy and anticonvulsant drugs.

Young¹,

Gauthier²,

Anderson³

et al. 1980

Journal of Neurology, Neurosurgery & Psychiatry

View full text Add to dashboard Cite

SUMMARY Tryptophan, 5-hydroxyindoleacetic acid and indoleacetic acid were measured in cerebrospinal fluid, taken during pneumoencephalography, from a large series of patients, the majority of whom were epileptics, most of them receiving anticonvulsants. CSF indoleacetic acid reflects CNS tryptamine metabolism in the same way that CSF 5-hydroxyindoleacetic acid reflects CNS 5-hydroxytryptamine metabolism. Our data suggest that (i) the brain tryptophan content is an important factor in the control of both 5-hydroxytryptamine and tryptamine synthesis (ii) brain 5-hydroxytryptamine metabolism exhibits a U-shaped relationship with age (iii) the mean brain tryptophan content and rate of 5-hydroxytryptamine metabolism are greater for women than men (iv) indoleamine metabolism is unaffected in untreated epileptics compared with non-epileptics, but anticonvulsant drugs decrease the rate of 5-hydroxytryptamine metabolism.Measurement of amine related compounds in the cerebrospinal fluid (CSF) is the most commonly used method for studying biogenic amine metabolism in the human CNS. For the indoleamines, the concentration of tryptophan in the CSF is a reasonably good index of the CNS tryptophan content,1 while the concentration of 5-hydroxyindoleacetic acid (5HIAA) in the CSF reflects CNS 5-hydroxytryptamine (5HT)

show abstract

Section: Age Differencesmentioning

confidence: 52%

Tryptophan, 5-hydroxyindoleacetic acid and indoleacetic acid in human cerebrospinal fluid: interrelationships and the influence of age, sex, epilepsy and anticonvulsant drugs.

Young¹,

Gauthier²,

Anderson³

et al. 1980

Journal of Neurology, Neurosurgery & Psychiatry

View full text Add to dashboard Cite

show abstract

“…Furthermore the negligible increase in brain 5-HIAA found when tryptophan was given to rats with decreased brain 5-HT may explain why the concentration of 5-HIAA in the CSF did not rise when L-tryptophan (2 to 3 g in divided doses) was given to a small group of depressives but did in other subjects (Bowers, 1970). This difference was not found when a somewhat larger single dose was given in another study (Ashcroft, Crawford, Cundall, Davidson, Dobson, Dow, Eccleston, Loose & Pullar, 1973).…”

Section: Discussionmentioning

confidence: 95%

5‐hydroxytryptamine: The Effects of Impaired Synthesis on Its Metabolism and Release in Rat

Curzon¹,

Fernando²,

Marsden

1978

British J Pharmacology

View full text Add to dashboard Cite

1 Control rats given L-tryptophan (100 mg/kg) showed a smaller increase of brain 5-hydroxytryptamine (5-HT) than its metabolite 5-hydroxyindoleacetic acid (5-HIAA). However, when brain 5-HT concentrations were depleted by 40-50% after treatment with the synthesis inhibitor p-chlorophenylalanine (PCPA) (150 mg/kg) L-tryptophan caused a considerable increase in 5-HT but no change in 5-HIAA. Similar results were obtained following depletion of brain 5-HT by pretreatment with p-chloroamphetamine (10 mg/kg). 2 Electrical stimulation of the median raphe nucleus of control rats significantly increased 5-HIAA in the hypothalamus, hippocampus and striatum. However, stimulation of PCPA (200mg/kg) pretreated animals did not significantly increase 5-HlAA either 24 or 72 h after administration of the drug. 3 Pretreatment of rats with PCPA (200 mg/kg) increased striatal synaptosomal uptake of [3H]-5HT by 30% and reduced 5-HT concentration in the rest of the brain by 62%. 4 PCPA (150mg/kg) markedly reduced the acute behavioural response (-76%) to p-chloroamphetamine (10 mg/kg) although brain 5-HT was only moderately reduced (-36%). L-Tryptophan (100 mg/kg) given 15 min before p-chloroamphetamine restored both brain 5-HT and the behavioural effects of p-chloroamphetamine in PCPA pretreated rats and enhanced the behavioural response to p-chloroamphetamine in control rats. 5The results suggest that newly synthesized 5-HT is less rapidly metabolized in rats with low brain 5-HT. The possible reasons for this and the relevance of the results to the use of L-tryptophan in the treatment of depressive illness are discussed.

show abstract

“…Previous research reported that tryptophan concentration in CSF reflects Trp content in brain tissue [14,49,58] and that the concentration of 5-HIAA in the lumbar CSF reflects the turnover of 5-HT in the central nervous system [2,13,14,20,39,57]. Still debated is the origin of tryptophan metabolites via serotonin in CSF.…”

Section: Discussionmentioning

confidence: 99%

A Study of Tryptophan Metabolism via Serotonin in Ventricular Cerebrospinal Fluid in HIV-1 Infection Using a Neuroendoscopic Technique

Longatti¹,

Perin²,

Comai³

et al. 2007

CHR

View full text Add to dashboard Cite

In this paper we report the study of tryptophan metabolism via serotonin in ventricular CSF in HIV-1 infection in order to investigate the origin of tryptophan metabolites in the human brain. The patients (n=4) were affected with non-communicating hydrocephalus. One of these also was suffering from HIV-1 infection. The CSF was withdrawn from different sites of the cerebral cavity with a neuroendoscopic procedure which allows an accurate exploration of all the cerebral ventricles. The measurement of tryptophan, 5-hydroxytryptophan, serotonin, 5-hydroxyindoleacetic acid, and melatonin was carried out by HPLC with fluorometric detection. In HIV-1 infection the highest concentration of tryptophan is present in the CSF of the choroid plexus; however, the levels are markedly lower than those in hydrocephalic individuals (control group). 5-Hydroxytryptophan CSF content is higher in HIV-1 infection than in hydrocephalic controls in all districts examined. Regarding serotonin, a great difference appears in the choroid plexus and in the pituitary recess between the HIV-1 infected patient and the control group. The values of 5-hydroxyindoleacetic acid are much lower in the CSF of the HIV-1 infected patient than in hydrocephalic controls. Melatonin levels appear to fluctuate largely but, in the HIV-1 infection, a great variability is present among the sites of CSF withdrawal. The third ventricle contains the highest concentration of melatonin and the choroid plexus and the pituitary recess the lowest. All the melatonin concentrations in HIV-1 infection are largely different than in hydrocephalic controls. This is the first report on the measurement of tryptophan metabolites via serotonin in ventricular CSF in HIV-1 infection.

show abstract

5-Hydroxytryptamine metabolism in affective illness: the effect of tryptophan administration

Cited by 51 publications

References 15 publications

Tryptophan, 5-hydroxyindoleacetic acid and indoleacetic acid in human cerebrospinal fluid: interrelationships and the influence of age, sex, epilepsy and anticonvulsant drugs.

Tryptophan, 5-hydroxyindoleacetic acid and indoleacetic acid in human cerebrospinal fluid: interrelationships and the influence of age, sex, epilepsy and anticonvulsant drugs.

5‐hydroxytryptamine: The Effects of Impaired Synthesis on Its Metabolism and Release in Rat

A Study of Tryptophan Metabolism via Serotonin in Ventricular Cerebrospinal Fluid in HIV-1 Infection Using a Neuroendoscopic Technique

Contact Info

Product

Resources

About