5-Hydroxytryptamine - a local regulator of testicular blood flow and vasomotion in rats

Collin, Ola; Damber, Jan Erik; Bergh, Anders

doi:10.1530/jrf.0.1060017

Cited by 41 publications

(26 citation statements)

References 7 publications

(15 reference statements)

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“…This technology has been previously used in the measurement of testicular [7] and mammary [8] blood flow in the rat. The FC 302 laser Doppler flow detection probe (PeriMed, Stockholm, Sweden) was positioned at the base of the penis lateral to the cavernosal vein of the left corpus cavernosum.…”

Section: Measurement Of Intracavernosal Blood Flow (Ccf)mentioning

confidence: 99%

Androgenic Maintenance of Inflow and Veno-Occlusion during Erection in the Rat

Mills

Lewis

Stopper

1998

Biology of Reproduction

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Ongoing studies in this laboratory are designed to determine the role of androgens in the maintenance of the erectile response in the rat. Testosterone-treated castrated rats (TESTO) and untreated castrated rats (CASTRATE) were used for measurement of the rate at which blood flows into the cavernous sinuses by timed collections of blood after partial amputation of the penis. A laser Doppler flow meter was employed to determine whether androgens also regulate the veno-occlusive mechanism that controls the rate of blood flow out of the sinuses. Erection was induced by direct electrical stimulation of the autonomic ganglion that controls cavernosal blood flow in the erectile response. The results of these studies showed that blood flow into the sinuses was approximately twice as great in the TESTO animals as the CASTRATE rats. Furthermore, during ganglionic stimulation, veno-occlusion occurred in the TESTO rats but failed to occur in the CASTRATE rats. The dependence of these responses on nitric oxide (NO) was demonstrated by showing that injection of sodium nitroprusside (SNP) enhances the intracavernosal pressure response in TESTO rats but not CASTRATE animals. However, when SNP injection was combined with ganglionic stimulation, veno-occlusion did occur in the CASTRATE animals. Taken together, these studies show that both the rate of blood flow into the cavernous sinuses and the blood flow out are under androgenic regulation and may involve the actions of NO.

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Section: Measurement Of Intracavernosal Blood Flow (Ccf)mentioning

confidence: 99%

Androgenic Maintenance of Inflow and Veno-Occlusion during Erection in the Rat

Mills

Lewis

Stopper

1998

Biology of Reproduction

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“…5-HT induces the contraction of the vas deferens (Hay & Wadsworth 1982) and regulates testicular blood flow (Collin et al 1996), testicular growth, cAMP production, and testosterone production , Frungieri et al 2002.…”

Section: Introductionmentioning

confidence: 99%

Evidence of 5-HT components in human sperm: implications for protein tyrosine phosphorylation and the physiology of motility

Jiménez-Trejo¹,

Tapia-Rodríguez²,

Cerbón³

et al. 2012

Reproduction

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Serotonin (5-hydroxytryptamine; C 10 H 12 N 2 O (5-HT)) is produced in the CNS and in some cells of peripheral tissues. In the mammalian male reproductive system, both 5-HT and tryptophan hydroxylase (TPH) have been described in Leydig cells of the testis and in principal cells of the caput epididymis. In capacitated hamster sperm, it has been shown that 5-HT promotes the acrosomal reaction. The aim of this work was to explore the existence of components of the serotoninergic system and their relevance in human sperm physiology. We used both immunocytochemistry and western blot to detect serotoninergic markers such as 5-HT, TPH1, MAO A , 5-HT 1B , 5-HT 3 , and 5HT T ; HPLC for TPH enzymatic activity; Computer Assisted Semen Analysis assays to measure sperm motility parameters and pharmacological approaches to show the effect of 5-HT in sperm motility and tyrosine phosphorylation was assessed by western blot. We found the presence of serotoninergic markers (5-HT, TPH1, MAO A , 5-HT 1B , 5-HT 2A , 5-HT 3 , 5-HT T , and TPH enzymatic activity) in human sperm. In addition, we observed a significant increase in tyrosine phosphorylation and changes in sperm motility after 5-HT treatment. In conclusion, our data demonstrate the existence of components of a serotoninergic system in human sperm and support the notion for a functional role of 5-HT in mammalian sperm physiology, which can be modulated pharmacologically.

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“…Serotonin has some effects known to be relevant to testis physiology. As mentioned above, serotonin inhibits testosterone synthesis (Tinajero et al 1993), functions as a potent vasoconstrictor of the testicular subcapsular artery at physiological and elevated transmural pressures (Davis 1992) and decreases the blood flow and vasomotion (Collin et al 1996). The effects of an elevated testicular blood pressure include focal apoptosis of spermatogonia and primary spermatocytes (Bergh et al 2001); whereas, a reduction in testicular blood flow could induce testicular hyperemia and edema (Davis 1992).…”

Section: Discussionmentioning

confidence: 99%

“…Still others have introduced the idea that serotonin modulates testosterone production (Tinajero et al 1993) and blood flow (Collin et al 1996) in the testis. However, the role of serotonin, if any, in the control of testis functions remains unknown.…”

Section: Introductionmentioning

confidence: 99%

Serotoninergic system blockage in the prepubertal rat inhibits spermatogenesis development

Martínez¹,

Ayala²,

Marin³

et al. 2005

Reproduction

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The stimulatory and inhibitory role of serotonin in gonadotropin secretion and in the onset of puberty in the male rat has been previously described, but its role in the establishment of spermatogenesis is not known. The aim of this study was to investigate the effects of serotoninergic inhibition by p-chloroamphetamine (pCA) on the prepubertal-to-adult stage of the rat reproductive system. Hypothalamic serotonin, gonadotropins and sex steroid hormone concentrations were measured, and a histopathological analysis of seminiferous epithelium was carried out on animals treated with pCA from day 30 and killed at 45 or 65 days of age. The pCA treatment significantly reduced the hypothalamic levels of serotonin and its metabolite (5-hydroxyindole-3-acetic acid). This inhibition did not affect the sex steroid hormone or LH concentrations, but rather it induced an increase in FSH concentration in animals of both ages. Spermatogenesis was impaired by pCA treatment. Disruption of seminiferous epithelium and the death of numerous germ cells were observed. Sperm produced by pCA-treated animals was of poor quality and appeared in small quantities. Apparently, serotonin depletion did not affect communication between the hypothalamus and the pituitary, but the FSH increase could have been related to alterations in the seminiferous epithelium effects. The seminiferous epithelium cycle was altered in rats killed at both 45 and 65 days of age, because at each age of killing the distribution of spermatogenesis stages was different. Germ cell apoptosis did not appear to be related to changes in the FSH concentrations, but other factors produced during spermatogenesis could have been involved in this induction. This study showed that serotonin was necessary for the development of normal spermatogenesis in prepubertal rats. Reproduction (2005) 129 717-727

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5-Hydroxytryptamine - a local regulator of testicular blood flow and vasomotion in rats

Abstract: The effects of 5-hydroxytryptamine (5-HT), 5-HT2 receptor antagonists (

Cited by 41 publications

References 7 publications

Androgenic Maintenance of Inflow and Veno-Occlusion during Erection in the Rat

Androgenic Maintenance of Inflow and Veno-Occlusion during Erection in the Rat

Evidence of 5-HT components in human sperm: implications for protein tyrosine phosphorylation and the physiology of motility

Serotoninergic system blockage in the prepubertal rat inhibits spermatogenesis development

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