5-HT1A-like receptor activation inhibits abstinence-induced methamphetamine withdrawal in planarians

Shah, Hardik; Ayoub, George; Raffa, Robert B.

doi:10.1016/j.neulet.2010.08.027

Cited by 9 publications

(3 citation statements)

References 37 publications

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“…Smith and Walker, 1974;Tembe et al, 1993;Ali and Orchard, 1994;Zhang and Harris-Warrick, 1994;Leake and Koubanakis, 1995;Dumitriu et al, 2006;Inohara et al, 2015) and behaviour (e.g. Yeh et al, 1997: Tierney et al, 2004Johnson et al, 2009;Rawls et al, 2010). Receptor subtypes identified in such studies often rely on drug application alone and must be viewed as provisional given the lack of specificity shown by available ligands and uncertainty about the site(s) where drugs acted to produce observable effects.…”

Section: Linking Heterologously Expressed Receptors To Endogenous Recmentioning

confidence: 99%

Invertebrate serotonin receptors: a molecular perspective on classification and pharmacology

Tierney

2018

Journal of Experimental Biology

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Invertebrate receptors for the neurotransmitter serotonin (5-HT) have been identified in numerous species from diverse phyla, including Arthropoda, Mollusca, Nematoda and Platyhelminthes. For many receptors, cloning and characterization in heterologous systems have contributed data on molecular structure and function across both closely and distantly related species. This article provides an overview of heterologously expressed receptors, and considers evolutionary relationships among them, classification based on these relationships and nomenclature that reflects classification. In addition, transduction pathways and pharmacological profiles are compared across receptor subtypes and species. Previous work has shown that transduction mechanisms are well conserved within receptor subtypes, but responses to drugs are complex. A few ligands display specificity for different receptors within a single species; however, none acts with high specificity in receptors across different species. Two non-selective vertebrate ligands, the agonist 5-methoxytryptamine and antagonist methiothepin, are active in most receptor subtypes in multiple species and hence bind very generally to invertebrate 5-HT receptors. Future challenges for the field include determining how pharmacological profiles are affected by differences in species and receptor subtype, and how function in heterologous receptors can be used to better understand 5-HT activity in intact organisms.

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Section: Linking Heterologously Expressed Receptors To Endogenous Recmentioning

confidence: 99%

Invertebrate serotonin receptors: a molecular perspective on classification and pharmacology

Tierney

2018

Journal of Experimental Biology

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“…In planarian D. japonica, four serotonin G-protein coupled receptors were found, the nucleotide sequences of which were established and have significant homology with the 5-HT 1A human serotonin receptor and Drosophila 5-HTdrol receptor. The planarian serotonin receptors were named 5-HTLpla1-4 [102][103][104]. Another receptor (DjSER7) was isolated from the planarian D. japonica demonstrating a high affinity for serotonin when incorporated into Xenopus oocytes [105].…”

Section: Discussionmentioning

confidence: 99%

Serotonin Signalling in Flatworms: An Immunocytochemical Localisation of 5-HT7 Type of Serotonin Receptors in Opisthorchis felineus and Hymenolepis diminuta

2021

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The study is dedicated to the investigation of serotonin (5-hydroxytryptamine, 5-HT) and 5-HT7 type serotonin receptor of localisation in larvae of two parasitic flatworms Opisthorchis felineus (Rivolta, 1884) Blanchard, 1895 and Hymenolepis diminuta Rudolphi, 1819, performed using the immunocytochemical method and confocal laser scanning microscopy (CLSM). Using whole mount preparations and specific antibodies, a microscopic analysis of the spatial distribution of 5-HT7-immunoreactivity(-IR) was revealed in worm tissue. In metacercariae of O. felineus 5-HT7-IR was observed in the main nerve cords and in the head commissure connecting the head ganglia. The presence of 5-HT7-IR was also found in several structures located on the oral sucker. 5-HT7-IR was evident in the round glandular cells scattered throughout the larva body. In cysticercoids of H. diminuta immunostaining to 5-НТ7 was found in flame cells of the excretory system. Weak staining to 5-HT7 was observed along the longitudinal and transverse muscle fibres comprising the body wall and musculature of suckers, in thin longitudinal nerve cords and a connective commissure of the central nervous system. Available publications on serotonin action in flatworms and serotonin receptors identification were reviewed. Own results and the published data indicate that the muscular structures of flatworms are deeply supplied by 5-HT7-IR elements. It suggests that the 5-HT7 type receptor can mediate the serotonin action in the investigated species and is an important component of the flatworm motor control system. The study of the neurochemical basis of parasitic flatworms can play an important role in the solution of fundamental problems in early development of the nervous system and the evolution of neuronal signalling components.

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“…pLMV was quantified as the number of gridlines crossed or recrossed during a 10 min observation period. Normal planarian movement consists of constant-velocity, horizontal, and forward-directed movement, with periodic turns, but rarely any stops [ 27 ].…”

Section: Methodsmentioning

confidence: 99%

Sertraline Induces Toxicity and Behavioral Alterations in Planarians

Thumé

Frizzo

2017

BioMed Research International

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Toxicity attributed to sertraline has been demonstrated recently in different cell types and also in some organisms. We investigated the effect of sertraline on planarians, which are considered suitable for investigations in neurotoxicology and currently are widely used as an animal model in neuropharmacological studies. Planarians treated with 10 µM sertraline showed a rapid reduction in their spontaneous movement until they became completely motionless and then showed a series of asynchronous paroxysms (seizures) followed by progressive tissue damage, beginning 48 h after the sertraline treatment, and died approximately 72 h later. Our data showed that sertraline does not cause planarian death within the range of therapeutic concentrations; however, behavioral alterations were observed with concentrations that can be considered compatible with therapeutic ones, such as a significant reduction in planarian locomotory activity at 0.4 µM. Treatment with 4 µM sertraline had a significant effect, reducing planarian locomotory activity and increasing the number of asynchronous paroxysms; both effects were significantly maintained even 24 h after the sertraline was withdrawn. These behavioral changes observed at low micromolar concentrations suggest that sertraline might have residual biological consequences for planarians, even after it is withdrawn.

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5-HT1A-like receptor activation inhibits abstinence-induced methamphetamine withdrawal in planarians

Cited by 9 publications

References 37 publications

Invertebrate serotonin receptors: a molecular perspective on classification and pharmacology

Invertebrate serotonin receptors: a molecular perspective on classification and pharmacology

Serotonin Signalling in Flatworms: An Immunocytochemical Localisation of 5-HT7 Type of Serotonin Receptors in Opisthorchis felineus and Hymenolepis diminuta

Sertraline Induces Toxicity and Behavioral Alterations in Planarians

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