5-FU and ixabepilone modify the microRNA expression profiles in MDA-MB-453 triple-negative breast cancer cells

Abstract

This study aimed to discover new potential mechanisms of chemotherapy with drugs used in the treatment of luminal androgen receptor (LAR)-type triple-negative breast cancer (TNBC). We examined the microRNA (miRNA) expression profiles of LAR-type TNBC in vitro, and explored the variation in miRNA expression profiles in cells when treated with the chemotherapy drugs capecitabine and ixabepilone. The present study revealed that the expression levels of the three antitumor miRNAs, miR-122a, miR-145 and miR-205, were significantly elevated in MDA-MB-453 LAR-type TNBC tumor cells treated with 5-fluorouracil together with ixabepilone. By contrast, carcinogenic miR-296 miRNA expression significantly declined, and levels of several other miRNAs such as miR-221, miR-210, miR-21 and miR-10b were also altered. The drugs may exert their effects through the regulation of miRNA expression levels, thereby providing a theoretical basis for clinical implementation of miRNA expression profiles as a diagnostic method for the early diagnosis, classification and prognosis of breast cancer.

MicroRNA expression profiling identifies miR-31-5p/3p as associated with time to progression in wild-type RAS metastatic colorectal cancer treated with cetuximab

“…From this perspective, is seems that miR-31-5p/3p could associated more probably with irinotecan then cetuximab response. Moreover, there is an increasing evidence describing link between chemotherapy and miRNAs regulatory network [ 10 , 11 ] indicating miRNAs involvement in chemosensitivity or chemoresistance [ 12 ]. On other hand, this evidence did not show any specific associations of miR-31-5p/3p and chemosensitivity, even in case of topoisomerase inhibitors like irinotecan (10–12).…”

5-Fluoruracil blocked giant cell tumor progression by suppressing osteoclastogenesis through NF-kappaB signals and blocking angiogenesis

“…As a commonly prescribed chemotherapy agents, its efficacy and safety have been proved in many clinical trials [24][25][26]. However, its inhibitory effect on bone tumor isn't often mentioned and the molecular mechanism behind this inhibition remains unexplored.…”

“…On the other side, in stromal cells, the expression of some cytokines is regulated by NF-κB, such as interleukin-1β (IL-1β), which can stimulate osteoclastogenesis and bone resorption in the presence of RANKL [7]; monocyte chemoattractant protein (MCP-1), which participates in unfavorable osteoclastogenesis and bone destruction [22]; and vascular endothelial cell growth factor (VEGF), which takes part in the process of angiogenesis [23]. , as a commonly used chemotherapy drug in clinical oncologic practice [24], is widely used for the treatment of various cancers, including gastrointestinal cancer, breast cancer, and neck cancer, and moreover, its efficacy and safety have been proved in many clinical trials [24][25][26]. It is well established that 5-FU targets a critical enzyme, thymidylate synthase (TS), which is the critical enzyme in DNA synthesis and repair [27].…”

Feature of Experimental Breast Cancer Induced by Intermammary Administration of N-Methyl-N-Nitrosourea in Wistar Rat

“…vitro 5-fluorouracil declined levels of miRNA-221, miRNA-210, miRNA-21 and miRNA-10b in luminal androgen receptor-type triple-negative breast cancer MDA-MB-453 cell line [31]. Cytokines and growth factors mediate not only intercellular communication in the immune system, but also influenced on the other cell types [32][33].…”