5′-(CGA) _n sequence-assisted pH-controlled assembly of supramolecular DNA nanostructure

Abstract

Herein, the DNA strands containing 5′-(CGA)n and consecutive guanines are used to construct supramolecular DNA nanostructures that are size-controlled by pH values. Additionally, the introduction of thymine linkers within DNA nanostructures is necessary to maintain the stability of long-sized nanostructures. This work also demonstrates a method for accurately building DNA nanostructures.

Attenuation of c-Myc expression in breast cancer by hesperidin-mediated stabilization of its promoter proximal G quadruplex region

“…The analytes were spread evenly on the mica surface for 5-8 min. Subsequently, the mica surface was washed with Milli-Q water to wipe off the excess salt and nally dried in the air (31).…”

Characterization of Parallel-Stranded DNA Duplexes by Surface-Enhanced Raman Spectroscopy and Bromide-Modified Gold Nanoparticles

“…2,3 Additionally, parallel dsDNA can be used in diagnostics and molecular assembly. 4,5 For example, autonomous DNA recognition systems employing parallel DNA duplexes facilitate the specific identification and diagnostic analysis of particular gene sequences. 6 The pH robust multimeric assembly of chimeric DNA composed of parallel dsDNAs and i-motif structures can be employed for the construction of nanodevices or therapeutic oligonucleotides.…”

“…However, in some cases, two DNA strands can also associate in the same direction forming parallel double-stranded DNA (dsDNA) (5′–3′/5′–3′), which are stabilized by Hoogsteen or other hydrogen bonds and base stacking, as shown in Figure . Parallel dsDNA has been shown to exist in biological genes and may play a key role in biological processes such as replication and transcription. , Additionally, parallel dsDNA can be used in diagnostics and molecular assembly. , For example, autonomous DNA recognition systems employing parallel DNA duplexes facilitate the specific identification and diagnostic analysis of particular gene sequences . The pH robust multimeric assembly of chimeric DNA composed of parallel dsDNAs and i-motif structures can be employed for the construction of nanodevices or therapeutic oligonucleotides …”