5-Aminosalicylic Acid attenuates allergen-induced airway inflammation and oxidative stress in asthma

Raju, Kalidhindi Rama Satyanarayana; Kumar, Monu; Gupta, Saurabh; Naga, Srinivas T.; Shankar, J; Murthy, Vishakantha; Madhunapanthula, Subba Rao V.; Mulukutla, Shashank; Ambhore, Nilesh Sudhakar; Tummala, Shashank; Vishnuvarthan, V.J.; Azam, Afzal; Elango, K

doi:10.1016/j.pupt.2014.07.007

Cited by 22 publications

(16 citation statements)

References 40 publications

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“…(Holgate et al, 2009;Prakash, 2013). Asthma is a multifaceted and intricate disease involving diverse pathologies, which makes it challenging to identify and address the core mechanisms involved (Hershenson et al, 2008;Raju et al, 2014;Sathish et al, 2015b;Prakash, 2016). Akin to clinical data, sex differences and sex steroids play a crucial role in the incidence and severity of asthma (Clough, 1993;Redline and Gold, 1994;Weiss and Gold, 1995;Caracta, 2003;Carey et al, 2007c;Card and Zeldin, 2009;Antunes et al, 2010;Woods et al, 2010;Townsend et al, 2012a;Bonds and Midoro-Horiuti, 2013;Sathish et al, 2015b;Sathish and Prakash, 2016).…”

Section: Discussionmentioning

confidence: 99%

“…Estimation of total and differential leukocyte count (DLC) in BALF was performed following previously published methods (Thompson et al, 1996;Raju et al, 2014;Ge et al, 2015;Ambhore et al, 2019a;Kalidhindi et al, 2019a). BALF was centrifuged at 2000 rpm for 5 min at 4°C and the supernatant was discarded.…”

Section: Total and Differential Leukocyte Count In Balfmentioning

confidence: 99%

“…Standard techniques were employed for histopathological studies using H&E for morphological analysis (Aravamudan et al, 2012;Raju et al, 2014;Liu et al, 2017;Ambhore et al, 2019a;Britt et al, 2019). For SRFG staining to detect collagen deposition standard technique was followed as per manufacturer's instructions (Chrondex, Inc., USA).…”

Section: Histopathology Using Hematoxylin and Eosin (Hande) And Sirius mentioning

confidence: 99%

“…Asthma is a chronic respiratory disorder causing significant morbidity and mortality worldwide. It is an intricate disorder involving diverse pathophysiologies affecting respiratory structure and thereby function (Townsend et al, 2012a;Prakash, 2013;Raju et al, 2014;Prakash, 2016;Raju et al, 2016). Asthma is characterized by inflammation and remodeling in the airways contributing to airway hyperresponsiveness (AHR) leading to episodic bronchoconstriction's (Holgate et al, 2009;Prakash, 2013;Prakash and Martin, 2014;Sathish et al, 2015b;Prakash, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Role of Estrogen Receptors α and β in a Murine Model of Asthma: Exacerbated Airway Hyperresponsiveness and Remodeling in ERβ Knockout Mice

et al. 2020

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Epidemiological data suggests increased prevalence of asthma in females than males, suggesting a plausible role for sex-steroids, especially estrogen in the lungs. Estrogen primarily acts through estrogen-receptors (ERa and ERb), which play a differential role in asthma. Our previous studies demonstrated increased expression of ERb in asthmatic human airway smooth muscle (ASM) cells and its activation diminished ASM proliferation in vitro and airway hyperresponsiveness (AHR) in vivo in a mouse (wild-type, WT) model of asthma. In this study, we evaluated the receptor specific effect of circulating endogenous estrogen in regulating AHR and remodeling using ERa and ERb knockout (KO) mice. C57BL/6J WT, ERa KO, and ERb KO mice were challenged intranasally with a mixedallergen (MA) or PBS. Lung function was measured using flexiVent followed by collection of broncho-alveolar lavage fluid for differential leukocyte count (DLC), histology using hematoxylin and eosin (H&E) and Sirius red-fast green (SRFG) and detecting asmooth muscle actin (a-SMA), fibronectin and vimentin expression using immunofluorescence (IF). Resistance (Rrs), elastance (Ers), tissue-damping (G) and tissue-elasticity (H) were significantly increased, whereas compliance (Crs) was significantly decreased in WT, ERa KO, and ERb KO mice (males and females) challenged with MA compared to PBS. Interestingly, ERb KO mice showed declined lung function compared to ERa KO and WT mice at baseline. MA induced AHR, remodeling and immune-cell infiltration was more prominent in females compared to males across all populations, while ERb KO females showed maximum AHR and DLC, except for neutrophil count. Histology using H&E suggests increased smooth muscle mass in airways with recruitment of inflammatory cells, while SRFG staining showed increased collagen deposition in MA challenged ERb KO mice compared to ERa KO and WT mice (males and females), with pronounced effects in ERb KO females. Furthermore, IF studies showed increased expression of a-SMA, fibronectin and vimentin in MA challenged populations compared to PBS, with prominent changes in ERb KO females. This novel study indicates ERb plays a pivotal role

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Section: Discussionmentioning

confidence: 99%

Section: Total and Differential Leukocyte Count In Balfmentioning

confidence: 99%

Section: Histopathology Using Hematoxylin and Eosin (Hande) And Sirius mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Role of Estrogen Receptors α and β in a Murine Model of Asthma: Exacerbated Airway Hyperresponsiveness and Remodeling in ERβ Knockout Mice

et al. 2020

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“…It is also a powerful scavenger of peroxy radical (Pearson et al, 1996). Raju et al (2014) evaluated the effect of 5-ASA on airway inflammation and oxidative stress in ovalbumin (OVA) induced allergic asthma in Balb/C mice. Treatment with oral 5-ASA (65, 130 and 195 mg/kg) for 6 days significantly reversed the OVA induced total and differential leucocyte count, IL-6, TNF-α, IL-13, nitrite and nitrate levels in BALF.…”

Section: Newer Targets For Respiratory Disordersmentioning

confidence: 99%

Status of Research in Respiratory Pharmacology in India during the last Five Years (2012-2017)

Gulati¹,

Rai²,

Ray³

2018

Proceedings of the Indian National Science Academy

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Research in the area of respiratory pharmacology and toxicologyis very exciting and vital for the development of new drugs with more efficacy and safety. Several medicinal plants from the Ayurveda and Unani systems of medicine are being evaluated for their efficacy and safety by using modern medical techniques;with an aim to bring them to the mainstream of the health care system. Effects of yogic intervention on pulmonary functions, cellular and molecular markers and quality of life in patients of bronchial asthma are also being assessed. Further, a lot of work is also going on novel aspects of immunotherapy and nanomedicine in the management of respiratory disorders. This review also highlights the relevance and pharmacoeconomic impact of nutraceuticals in prevention and treatment of respiratory diseases. Many clinical trials are being conducted to compare the efficacy and safety of already marketed individual drugs and combination therapy. Pharmacovigilance studies are being conducted to monitor the ADRs during pharmacotherapy of respiratory diseases, assess the causal relationship and find ways to prevent the ADRs to rationalize the therapy. However, more translational research is needed to bring scientific advances and knowledge into clinical practice; and there is a great need to find effective ways for collaboration between various research disciplines. There should be a focus on earlier and more specific diagnosis of respiratory diseases as well as better targeted and personalized treatments which may save cost, reduce adverse effects and improve disease outcomes and quality of life.

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Cellular and Biochemical Analysis of Bronchoalveolar Lavage Fluid from Murine Lungs

Kalidhindi

Ambhore

Sathish

2020

Methods in Molecular Biology

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5-Aminosalicylic Acid attenuates allergen-induced airway inflammation and oxidative stress in asthma

Cited by 22 publications

References 40 publications

Role of Estrogen Receptors α and β in a Murine Model of Asthma: Exacerbated Airway Hyperresponsiveness and Remodeling in ERβ Knockout Mice

Role of Estrogen Receptors α and β in a Murine Model of Asthma: Exacerbated Airway Hyperresponsiveness and Remodeling in ERβ Knockout Mice

Status of Research in Respiratory Pharmacology in India during the last Five Years (2012-2017)

Cellular and Biochemical Analysis of Bronchoalveolar Lavage Fluid from Murine Lungs

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