5-Aminolevulinic acid ester?induced protoporphyrin IX in a murine melanoma cell line

Vena, Flávia Cristina Bonilha; Turchiello, Rozane de Fátima; Laville, Isabelle; Pigaglio, Sophie; Blais, J.; Tedesco, Antônio Cláudio

doi:10.1007/s10103-004-0313-y

Cited by 16 publications

(10 citation statements)

References 33 publications

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“…As a further marker of differentiation, we monitored the production of PpIX. PpIX is a photosensitiser compound synthesized and localized in the mitochondria as intermediate molecules in the heme biosynthesis pathway, of which 5-aminolevulinic acid (ALA) is the first intermediate (31). The treated melanoma cells emitted a PpIX fluorescence higher than untreated cells probably due to a synthesis increase and intracellular accumulation of PpIX during chrysin treatment.…”

Section: Discussionmentioning

confidence: 99%

Chrysin-induced apoptosis is mediated through p38 and Bax activation in B16-F1 and A375 melanoma cells

Canini

2011

Int J Oncol

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Abstract. Chrysin (5,7-dihydroxyflavone) is a natural and biologically active compound extracted from honey, plants and propolis. It possesses anti-inflammatory activity, antioxidant properties and promotes cell death by perturbing cell cycle progression. In this study, our attention focused on the possible role that chrysin may have as a potential anti-cancer agent, and we tested its biological activity in murine and human melanoma cell lines (B16-F1 and A375). This study demonstrated that chrysin reduced melanoma cell proliferation and induced cell differentiation in both human and murine melanoma cells through synthesis increase and intracellular accumulation of protoporphirin IX (PpIX). Furthermore, following treatments with chrysin an increase in the expression of porphobilinogen deaminase (PBG-D) was noted. This study demontrated also that chrysin induces cell death in human and murine melanoma cells through caspase-dependent mechanisms, involving down-regulation of ERK 1/2, and activation of p38 MAP kinases. Induction of cell death may be a promising therapeutic approach in cancer therapy. Our results suggest that chrysin may be considered a potential candidate for both cancer prevention and treatment.

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Section: Discussionmentioning

confidence: 99%

Chrysin-induced apoptosis is mediated through p38 and Bax activation in B16-F1 and A375 melanoma cells

Canini

2011

Int J Oncol

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“…PpIX and PpIX dimethyl ester, a potential PAP) after exposing cells to MAL. Furthermore, Gaullier et al (50) and Vena et al (51) observed no differences in fluorescence localization after exposure to 5‐ALA or 5‐ALA esters at the cellular level.…”

Section: The Biochemistry Of 5‐ala and Its Derivativesmentioning

confidence: 96%

“…In an in vitro screening procedure Brunner et al (29) found that the dendron 5‐ALA conjugate (51) was more effective than the tetramer (53) in terms of PAP induction capacity at equimolar doses. Furthermore, compound 51 has been shown to induce fluorescence intensity in J82 and HT29 cells that is 3.5 times higher than that induced by 5‐ALA. In contrast, Di Venosa (110) found similar in vitro activity of a tetrameric 5‐ALA dendron, compared with 5‐ALA, in LM3 cells.…”

Section: Chemistry and Design Of 5‐ala Derivativesmentioning

confidence: 99%

5‐Aminolevulinic Acid Derivatives in Photomedicine: Characteristics, Application and Perspectives

Fotinos

Campo

Popowycz

et al. 2006

Photochem & Photobiology

206

128

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The introduction of lipophilic derivatives of the naturally occurring heme precursor 5-aminolevulinic acid (5-ALA) into photomedicine has led to a true revival of this research area. 5-ALA-mediated photodynamic therapy (PDT) and fluorescence photodetection (FD) of neoplastic disease is probably one of the most selective cancer treatments currently known in oncology. To date, this method has been assessed experimentally for the treatment of various medical indications. However, the limited local bioavailability of 5-ALA has widely prevented its use in daily clinical practice. Although researchers were already aware of this drawback early during the development of 5-ALA-mediated PDT, only recently have well-established concepts in pharmaceutical science been adapted to investigate ways to overcome this drawback. Recently, two derivatives of 5-ALA, methylaminolevulinate (MAL) and hexylaminolevulinate (HAL), gained marketing authorization from the regulatory offices in Europe and Australia. MAL is marketed under the trade name Metvix for the treatment of actinic keratosis and difficult-to-treat basal cell carcinoma. HAL has recently been launched under the trade name Hexvix to improve the detection of superficial bladder cancer in Europe. This review will first present the fundamental concepts underlying the use of 5-ALA derivatives in PDT and FD from a chemical, biochemical and pharmaceutical point of view. Experimental evidences from preclinical data on the improvements and limits observed with 5-ALA derivatives will then be introduced. The state-of-the-art from clinical studies with 5-ALA esters will be discussed, with special emphasis placed on the process that led to the development of MAL in dermatology and to HAL in urology. Finally, we will discuss promising medical fields in which use of 5-ALA derivatives might potentially lead to further use of this methodology in photomedicine.

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“…The choice of the source of light must be based on the absorption by the photosensitizer drug, the purpose of the action (treatment of a small cancerous lesion or wound healing stimulation), characteristics of the lesion or wound (local, size, accessibility, and characteristics of the tissue) costs and size [7]. A wide number of photosensitizers in different drug delivery systems (DDSs) have been tested for PDT [25][26][27][28][29]. Among them, phthalocyanines (Pc) have been under investigation as promising second-generation photosensitizers, not only for PDT, but also for other applications.…”

Section: Interaction Of Light In the Skinmentioning

confidence: 99%

Low Level Energy Photodynamic Therapy for Skin Processes and Regeneration

Tedesco¹,

Jesus²

2017

Photomedicine - Advances in Clinical Practice

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Skin is the largest human organ and displays multiple functions involving structure and protection against external agents that may affect the body. Solar radiation accelerates the normal aging process and may even cause great damage leading to many different cutaneous diseases and skin cancer. Moreover, a wound in the skin may be an open channel for the access of pathogens usually exposing blood vessels for infections and causing serious complications. For many reasons, regulatory skin processes are of great deal in different approaches: basic antiaging, wound healing, and skin cancer. In a good way, photoprocesses with specific wavelength at low energy levels associated with photoactive compounds are known to cause the opposite effect, promoting the healing of cutaneous diseases and leading to well-defined outcomes in rejuvenation and antiaging. This chapter will discuss the most relevant topics in photo skin regeneration using low energy levels associated with photodynamic therapy (PDT), which emerged as a combination to potentialize molecules with the already known effects of PDT and low level laser therapy (LLLT) in the treatment of skin pathologies, known as a photobiomodulation process.

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5-Aminolevulinic acid ester?induced protoporphyrin IX in a murine melanoma cell line

Cited by 16 publications

References 33 publications

Chrysin-induced apoptosis is mediated through p38 and Bax activation in B16-F1 and A375 melanoma cells

Chrysin-induced apoptosis is mediated through p38 and Bax activation in B16-F1 and A375 melanoma cells

5‐Aminolevulinic Acid Derivatives in Photomedicine: Characteristics, Application and Perspectives

Low Level Energy Photodynamic Therapy for Skin Processes and Regeneration

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