5-Aminolaevulinic acid (ALA) induced formation of different fluorescent porphyrins: A study of the biosynthesis of porphyrins by bacteria of the human digestive tract

Dietel, W.; Pottier, R.; Pfister, W.; Schleier, Peter; Zinner, Karen

doi:10.1016/j.jphotobiol.2006.07.006

Cited by 49 publications

(38 citation statements)

References 13 publications

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“…In minced pork meat, a peak at 590 nm was identified as ZnPP and two others at 636 and 705 nm as PP by Veberg et al (2006). Indeed as described by Dietel et al (2007) PP is also produced in the biosynthetic pathway from 5-Aminolaevulinic acid to heme in all living cells including those of microorganisms such as enterobacteria which play an important role in the contamination of meat.…”

Section: Fluorescence Investigationsmentioning

confidence: 96%

Non-invasive mobile monitoring of meat quality

et al. 2009

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Detailed investigations into the time-dependent changes of pork meat quality and composition have been carried out using standard analytical techniques and Raman and fluorescence spectroscopy. Characteristic changes in soluble protein content, microbial load and biogenic amine content have been measured in the time period from slaughter up to 20 to 30 days post mortem on samples of porcine musculus longissimus dorsi. Characteristic behaviour indicative of a deterioration of meat quality and an increase in microorganism population on the meat surface has been observed. Fluorescence and Raman optical signals have been identified which change over this time period, correlating with the standard measurements and these can be used as indicators of meat properties. These signals can be measured rapidly and without the destruction of the meat sample allowing non-invasive testing. The combination of these two powerful measurement techniques provides a pathway to comprehensive meat quality monitoring. Furthermore we have developed a hardware concept for carrying out these spectroscopic measurements using a compact and light handheld device and we present concepts for the use of such a device in the logistic chain in combination with a transponder chip and integrated sensors which provide product identification and information about storage conditions along the entire logistic chain to the supermarket shelf

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Section: Fluorescence Investigationsmentioning

confidence: 96%

Non-invasive mobile monitoring of meat quality

et al. 2009

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“…A) is a well‐established permeability barrier and it has been proposed that this barrier may contribute to the higher levels of resistance shown by these organisms to ALA‐based PDT by inhibiting ALA uptake . However, in addition to the organization of their membrane systems, a number of other factors have been shown to contribute to the susceptibility of both Gram‐negative bacteria and Gram‐positive bacteria to ALA‐based PACT, including: membrane composition, levels of PPIX, and other porphyrins induced by ALA, the lipophilicity and concentrations of the exogenous ALA compounds utilized . Given these variables, it would seem clear that ALA‐based PDT conducted at suboptimal conditions may lead to misleading results, which may explain some apparently conflicting reports, which have appeared in the literature.…”

Section: The Inactivation Of Bacteria By Ala‐based Pdtmentioning

confidence: 99%

“…In addition to the mainstream PDT regime described above, a unique photodynamic antimicrobial strategy has been developed, which is based on the use of 5‐aminolevulinic acid (ALA), also known as δ‐aminolevulinic acid, δ‐aminolevulinate, 5‐amino‐4‐oxo‐pentanoic acid, and γ‐keto‐δamino valerate . ALA itself is not photodynamically active but when taken up by target cells, it can act as prodrug, inducing the accumulation of endogenous PS whose light activation can lead to a range of antimicrobial effects . Based on these observations, ALA‐based PDT has been increasingly researched for medical application in addition to a number of nonmedical uses .…”

Section: Introductionmentioning

confidence: 99%

Photodynamic therapy based on 5‐aminolevulinic acid and its use as an antimicrobial Agent

Harris

Pierpoint

2011

Medicinal Research Reviews

127

106

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Exogenous 5-aminolevulinic acid (ALA) is taken up directly by bacteria, yeasts, fungi, and some parasites, which then induces the accumulation of protoporphyrin IX (PPIX). Subsequent light irradiation of PPIX leads to the inactivation of these organisms via photodamage to their cellular structures. ALA uptake and light irradiation of PPIX produced by host cells leads to the inactivation of other parasites, along with some viruses, via the induction of an immune response. ALA-mediated PPIX production by host cells and light irradiation result in the inactivation of other viruses via either the induction of a host cell response or direct photodynamic attack on viral particles. This ALA-mediated production of light-activated PPIX has been extensively used as a form of photodynamic therapy (PDT) and has shown varying levels of efficacy in treating conditions that are associated with microbial infection, ranging from acne and verrucae to leishmaniasis and onychomycosis. However, for the treatment of some of these conditions by ALA-based PDT, the role of an antimicrobial effect has been disputed and in general, the mechanisms by which the technique inactivates microbes are not well understood. In this study, we review current understanding of the antimicrobial mechanisms used by ALA-based PDT and its role in the treatment of microbial infections along with its potential medical and nonmedical applications.

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“…Protoporphyrin IX accumulates in the cellular mitochondria, which is used in PDT. Combined with a light source with a wavelength corresponding to the absorption maximum of the photosensitizing substance, the process gives rise to reactive oxygen species triggering cell death [12,13].…”

Section: Photodynamic Therapy Withmentioning

confidence: 99%

“…W komórce, w mitochondriach dochodzi do gromadzenia PpIX, co wykorzystuje się w PDT. W połącze-niu ze źródłem światła o długości fali odpowiadającej maksimum absorpcji dla substancji fotoczułej w procesie tym wytwarzają się reaktywne formy tlenu, któ-re powodują obumieranie komórki [12,13]. Do przeprowadzenia PDT niezbędne są trzy skład-niki -fotouczulacz, tlen oraz źródło światła.…”

Section: Photodynamic Therapy Withunclassified

Photodynamic therapy in the treatment of onychomycosis – a review

Kalinowska¹,

Hryncewicz-Gwóźdź²

2017

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Toenail onychomycosis is a common infection, affecting up to 14% of the population. Moreover, poor penetration of topical drugs into the nail bed leads to frequent recurrences of the disease. The cure rate of toenail onychomycosis is estimated at 63-76%. An alternative treatment for onychomycosis is photodynamic therapy. Following agents are used in this method: porfimer sodium, rose bengal, methylene blue, 5-aminolevulinic acid and its derivative -methyl ester. The therapy is well tolerated Photodynamic therapy offers an opportunity to achieve a significant cure rate in patients who cannot receive oral therapy. Thanks to systematic improvements in photodynamic therapy, including better photosensitizer penetration into the nail bed and shorter exposure time, photodynamic therapy may be more efficient and cost-effective than other onychomycosis treatments. However, randomized clinical trials evaluating the efficacy of photodynamic therapy of onychomycosis are needed. STRESZCZENIEGrzybica paznokci stóp jest częstym zakażeniem, może dotyczyć do 14% populacji. Ponadto utrudnione przenikanie substancji leczniczych do łożyska paznokcia w leczeniu miejscowym powoduje częste nawroty schorzenia. Szacuje się, że odsetek wyleczeń grzybicy paznokci stóp wynosi 63-76%. Terapia fotodynamiczna stanowi alternatywną metodę leczenia grzybicy paznokci. W tym zabiegu wykorzystuje się następu-jące substancje: porfimer sodu, róż bengalski, błękit metylenowy, kwas 5-aminolewulinowy oraz jego pochodną -ester metylowy. Leczenie jest dobrze tolerowane przez pacjentów. Dzięki zastosowaniu terapii fotodynamicznej można uzyskać znaczny odsetek wyleczeń wśród pacjentów, którzy nie mogą przyjmować terapii doustnej. Dzięki systematycznemu ulepszaniu terapii fotodynamicznej związanemu z lepszą penetracją fotouczulacza do łożyska paznokcia czy też ze skróceniem czasu naświetlania może być ona bardziej wydajna i kosztować mniej w porównaniu z innymi metodami leczenia onychomykozy. Konieczne są jednak randomizowane badania kliniczne oceniające skuteczność leczenia grzybicy paznokci za pomocą terapii fotodynamicznej.

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5-Aminolaevulinic acid (ALA) induced formation of different fluorescent porphyrins: A study of the biosynthesis of porphyrins by bacteria of the human digestive tract

Cited by 49 publications

References 13 publications

Non-invasive mobile monitoring of meat quality

Non-invasive mobile monitoring of meat quality

Photodynamic therapy based on 5‐aminolevulinic acid and its use as an antimicrobial Agent

Photodynamic therapy in the treatment of onychomycosis – a review

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