5,6-Dihydrouridine: a marker ribonucleoside for determining whole body degradation rates of transfer RNA in man and rats

Topp, Heinrich; Duden, Rolf; Schöch, G.

doi:10.1016/0009-8981(93)90223-q

Cited by 28 publications

(23 citation statements)

References 15 publications

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“…Oxidized nucleosides are generated post-transcriptionally by various modification enzymes during RNA turnover, and they circulate in the blood and are excreted in urine (Topp et al, 1993;Nakano et al, 1993). Their levels are increased in a number of malignant diseases such as those associated with tumors, aging, carcinogenesis, and neurodegeneration, so they can be used in clinical chemistry as biomarkers (Itoh et al, 1992;Thomale and Nass, 1983;Fischbein et al, 1983;Speer et al, 1979;Zambonin et al, 1999;Abe et al, 2002Abe et al, , 2003.…”

Section: Introductionmentioning

confidence: 98%

Direct determination of nucleosides in the urine of patients with breast cancer using column-switching liquid chromatography–tandem mass spectrometry

Cho

Jung²,

Lee³

et al. 2006

Biomed. Chromatogr.

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We developed an analytical method for a simple, sensitive and simultaneous determination of oxidized nucleosides in urine using column-switching liquid chromatography-electrospray/tandem mass spectrometry (LC-ESI/MS/MS). We connected two columns through a six-way switching valve and effectively separated nucleosides in the urine from the interference by column-switching liquid chromatography. We monitored separated nucleosides using positive ionization tandem mass spectrometry in selective reaction monitoring (SRM) mode. The calibration ranges of nucleosides were 0.2-100 nmol/mL. The linearity of the method was 0.994-0.999, and the limits-of-detection (LOD) at a signal-to-noise (S/N) ratio of 3 were 0.1-0.2 nmol/mL. The coefficients of variation were in the range 2.28-11.74% for within-day variation and 4.36-11.15% for day-to-day variation, respectively. To explore the relationship between breast cancer and the nucleosides level in human urine, we measured the concentrations of nucleosides in female patients with breast cancer (n = 30) and in normal female subjects (n = 30). The concentration of nucleosides was significantly increased in patients with breast cancer when compared with the normal controls (1-methyladenosine; p < 0.005, N(2),N(2)-dimethylguanosine; p < 0.01, 5-hydroxymethyl-2'-deoxyuridine; p < 0.001, 8-hydroxy-2-deoxyguanosine; p < 0.001). Therefore, the elevated levels of nucleosides could be used as an important biomarker for breast-cancer research.

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Section: Introductionmentioning

confidence: 98%

Direct determination of nucleosides in the urine of patients with breast cancer using column-switching liquid chromatography–tandem mass spectrometry

Cho

Jung²,

Lee³

et al. 2006

Biomed. Chromatogr.

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“…tion markers for tRNA because they occur only in this RNA class (2,5). Urinary C originates from degraded rRNA and tRNA and negligibly from uridine-rich small nuclear riboWe developed a noninvasive method for determining nucleic acid (U-snRNA).…”

Section: Rna Classes Is Known (2 3; Cf Materials and Methods)mentioning

confidence: 99%

“…The culturcorrelations exist between the degradation rates of the ing of the cells has been described in detail elsewhere different RNA classes and the respective metabolic (17). Briefly, freshly prepared hepatocyte suspensions rates (3)(4)(5)(6). Based on these findings we have hypothe-were seeded on gas-permeable, collagen I (rat tail)-sized that in differently sized mammals at metabolic coated Petriperm dishes.…”

Section: Rna Classes Is Known (2 3; Cf Materials and Methods)mentioning

confidence: 99%

Determination of Degradation Rates of Transfer and Ribosomal Ribonucleic Acids in Cultured Rat Hepatocytes by MeasuringN6-Threoninocarbonyladenosine, Dihydrouridine, and Pseudouridine in Medium Using High-Performance Liquid Chromatography

Topp

Dörrenhaus

Bolt

et al. 1997

Analytical Biochemistry

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“…As such, tRNA modification has been considered essential for tRNA maturation and stability. Modified nucleosides contained in most tRNA have been associated with turnover (Topp et al 1993;Nakano et al 1993;Sander et al 1986); therefore, modified nucleosides, such as m 1 A, 2-methylguanosine (m 2 G), and pseudouridine (Ψ), have been reported to be cancer biomarkers (Itoh et al 1992;Jeng et al 2009;Djukovic et al 2010;Cho et al 2009). Recent studies have shown that altering the tRNA modification profile plays a major role in the cellular stress response (Chan et al 2010(Chan et al , 2012(Chan et al , 2015Endres et al 2015), where 5-methylcytidine (m 5 C), 2′-O-methylcytidine (Cm), and N2,N2-dimethylguanosine (m 2 2 G) reportedly increase under oxidative conditions created by H 2 O 2 ; other oxidative stress reagents do not influence these modified nucleosides (Chan et al 2010(Chan et al , 2012.…”

Section: Introductionmentioning

confidence: 99%

Rapid and selective simultaneous quantitative analysis of modified nucleosides using multi-column liquid chromatography-tandem mass spectrometry

et al. 2017

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Background: The profiles of modified nucleosides could act as useful biomarkers for cancer and cellular stressinduced diseases. However, there are no reports of high throughput and simultaneous quantitative methods for using biomarker evaluation and discovery at the bedside. Methods: Modified nucleosides were separated on two CAPCELL PAK ADME S3 (100 mm × 2.1 mm i.d.; 3-μm particle size) analytical columns coupled with a CAPCELL PAK ADME cartridge (10 mm × 2 mm i.d.; 3-μm particle size) guard column. Both columns were used in tandem during multi-column LC analysis to reduce analysis time. Two mobile phases were used, including 20 mM ammonium acetate adjusted to pH 5.3 using acetic acid and 1. 0 M ammonium acetate/acetonitrile/water/acetic acid (1/95/5/0.03, v/v/v/v), with the post-column addition of methanol to enhance ionization efficiency. Tandem mass spectrometry detection was performed using a triple quadrupole mass spectrometer equipped with a heated electrospray ionization source in selected reaction monitoring mode. Results: Four major nucleosides and 11 modified nucleosides, including guanosine, adenosine, uridine (U), cytidine, inosine, 1-methyladenosine, 5-methylcytidine, 2′-O-methylcytidine, 3-methylcytidine, 7-methylguanosine (m 7 G), 5-methyluridine (m 5 U), pseudouridine, 2-thiocytidine, N 2 -methylguanosine (m

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5,6-Dihydrouridine: a marker ribonucleoside for determining whole body degradation rates of transfer RNA in man and rats

Cited by 28 publications

References 15 publications

Direct determination of nucleosides in the urine of patients with breast cancer using column-switching liquid chromatography–tandem mass spectrometry

Direct determination of nucleosides in the urine of patients with breast cancer using column-switching liquid chromatography–tandem mass spectrometry

Determination of Degradation Rates of Transfer and Ribosomal Ribonucleic Acids in Cultured Rat Hepatocytes by MeasuringN6-Threoninocarbonyladenosine, Dihydrouridine, and Pseudouridine in Medium Using High-Performance Liquid Chromatography

Rapid and selective simultaneous quantitative analysis of modified nucleosides using multi-column liquid chromatography-tandem mass spectrometry

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