[48] Assay of glutathione, glutathione disulfide, and glutathione mixed disulfides in biological samples

Akerboom, Theodorus P.M.; Sies, Helmut

doi:10.1016/s0076-6879(81)77050-2

Cited by 1,385 publications

(606 citation statements)

References 33 publications

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“…23 The concentration of GSH in bile and perfusate was measured together with GSSG in a kinetic assay using NADPH, GSSG reductase, and 5,5-dithiobis(nitrobenzoic acid) (DTNB). 21 GSH values were obtained by the difference between total glutathione (GSH + GSSG) and GSSG.…”

Section: Methodsmentioning

confidence: 99%

Prevention of ischemia/reperfusion injury in the rat liver by atrial natriuretic peptide

Bilzer¹,

Witthaut²,

Paumgartner³

et al. 1994

Gastroenterology

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Section: Methodsmentioning

confidence: 99%

Prevention of ischemia/reperfusion injury in the rat liver by atrial natriuretic peptide

Bilzer¹,

Witthaut²,

Paumgartner³

et al. 1994

Gastroenterology

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“…The levels of GPx, GSH, CAT and SOD were measured in cytosolic fractions following the standard analytical methods of Flohe and Gunzler [20], Akerboom and Sies [21], Nelson and Kiesow [22] and Misra and Fridovich [23], respectively. The experiments were repeated at least three times.…”

Section: Assays For Antioxidant Enzymesmentioning

confidence: 99%

Obesity increases the risk of UV radiation-induced oxidative stress and activation of MAPK and NF-κB signaling

Katiyar

Meeran

2007

Free Radical Biology and Medicine

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Obesity has been implicated in several diseases including cancer, however, the relationship of obesity and susceptibility to ultraviolet (UV) radiation-caused skin diseases has not been investigated. As UV-induced oxidative stress has been implicated in several skin diseases, we assessed the role of obesity on UVB-induced oxidative stress in genetically obese Lep ob /Lep ob (leptin-deficient) mice. Here, we report that chronic exposure to UVB (120 mJ/cm 2 ) resulted in greater oxidative stress in the skin of obese mice in terms of higher levels of H 2 O 2 and NO production, photo-oxidative damage of lipids and proteins, and greater depletion of antioxidant defense enzymes, like, glutathione, glutathione peroxidase and catalase. As UV-induced oxidative stress mediates activation of MAPK and NF-κB signaling pathways, we determined the effect of UVB on these pathways in obese mice. Exposure of obese mice to UVB resulted in phosphorylation of ERK1/2, JNK and p38 proteins of the MAPK family. Compared to wild-type mice, the obese mice exhibited higher levels of phosphorylation of these proteins, greater activation of NF-κB/p65, and higher levels of circulating proinflammatory cytokines, including TNF-α, IL-1-β and IL-6, on UVB irradiation. Together, our study suggests for the first time that obesity in mice is associated with greater susceptibility to UVBinduced oxidative stress, and therefore may be a risk factor for skin diseases associated with UVBinduced oxidative stress.

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“…GSH was evaluated according to the method of Akerboom and Sies (1981) which was slightly modified and based on the spectrophotometric evaluation (Uvikon 860, Kontron Instruments, France) of the reduction rate of 5,5 0 -dithiobis-2-nitrobenzoic acid (DTNB) into 5-thio-2-nitrobenzoate (TNB) at 412 nm. Values were determined by comparing the reduction rate against a standard curve of GSH.…”

Section: Laboratory Assessmentmentioning

confidence: 99%

Age-related oxidative stress and antioxidant parameters in middle-aged and older European subjects: the ZENITH study

et al. 2005

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Objective: Oxidative stress has been reported to increase with ageing. However, the data in healthy humans remain controversial and studies in free-living elderly people are scarce. The objective of the present study was to compare age-related oxidative stress in late middle-aged and older free-living subjects. Design: The effect of ageing on oxidative stress and antioxidant parameters was investigated in 188 middle-aged subjects from Clermont-Ferrand (France) and Coleraine (UK), and in 199 older subjects from Grenoble (France) and Roma (Italy). Plasma thiol (SH) groups, define definition (TBAR's) and total glutathione (GSH), define definition (FRAP), and superoxide dismutase (SOD) activity were measured at baseline of the ZENITH study. Results: Plasma SH groups and FRAP and, surprisingly, TBAR's were significantly lower in free-living older subjects compared to younger subjects (Po0.001, Po0.001, Po0.01, respectively), but there was no significant differences in GSH levels. Conclusion: European free-living healthy older do not appear to be exposed to an acute oxidative stress. However, the highly significant positive correlation between plasma SH group oxidation or decreased FRAP and ageing is predictive of an increased risk of oxidative stress in older subjects. Moreover, the comparison between middle-aged and older subjects regarding oxidative stress parameters suggests also a progressive and slow decline of antioxidant status in healthy free-living older elderly and underline the impact on life-style factors on successful ageing.

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[48] Assay of glutathione, glutathione disulfide, and glutathione mixed disulfides in biological samples

Cited by 1,385 publications

References 33 publications

Prevention of ischemia/reperfusion injury in the rat liver by atrial natriuretic peptide

Prevention of ischemia/reperfusion injury in the rat liver by atrial natriuretic peptide

Obesity increases the risk of UV radiation-induced oxidative stress and activation of MAPK and NF-κB signaling

Age-related oxidative stress and antioxidant parameters in middle-aged and older European subjects: the ZENITH study

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