[45] ADP-ribosylation of membrane components by pertussis and cholera toxin

Ribeiro-Neto, Fernando; Mattera, Rafael; Hildebrandt, John D.; Codina, Juan; Field, James B.; Birnbaumer, Lutz; Sekura, Ronald D.

doi:10.1016/0076-6879(85)09115-7

Cited by 202 publications

(83 citation statements)

References 12 publications

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“…Thus, the low Ca++ bath + TMB-8 blocked PGE2's capacity to produce a sustained increase in intracellular calcium, and reversed the inhibitory effect of PGE2 on lumento-bath 22Na flux. (19,20). 100 ng/ml pertussis toxin partially reversed the inhibitory effect of 10-' M PGE2 on AVP-induced Lp from 91.4±14.1 (PGE2 alone) to 134.5±19.2 PT + PGE2 (Fig.…”

Section: Calculationsmentioning

confidence: 97%

Prostaglandin E2 inhibits sodium transport in rabbit cortical collecting duct by increasing intracellular calcium.

Hébert¹,

Jacobson²,

Breyer³

1991

J. Clin. Invest.

123

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Section: Calculationsmentioning

confidence: 97%

Prostaglandin E2 inhibits sodium transport in rabbit cortical collecting duct by increasing intracellular calcium.

Hébert¹,

Jacobson²,

Breyer³

1991

J. Clin. Invest.

123

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“…Non-specific binding was estimated by addition of 2 mM GTP. CT-and PT-mediated ADP-ribosylation, followed by immunoprecipitation with antiserum AS 231 or pre-immune serum, was performed as described previously [26][27][28]. Protein concentrations were determined by the method of Lowry et al [29] with modifications [30].…”

Section: Sucrose Density Gradientsmentioning

confidence: 99%

Purification of the G-protein G13 from rat brain membranes

Harhammer

Nürnberg

Spicher

et al. 1994

Biochemical Journal

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Significant amounts of G13, a member of the recently described G12-subfamily of heterotrimeric G-proteins, have been detected in rat brain membranes by specific antisera. The a-subunits of G13 (Gac3) were purified by using a combination of conventional and subunit-exchange chromatography. Purification was facilitated by the fact that the initial anion-exchange chromatography separated G13 from most of the other G-proteins, including Gq/11. Moreover, Ga13-enriched fractions obtained from this chromatographic step were devoid of fly-dimers, despite

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“…BBM (5 mg protein) were incubated with a ribosylation buffer containing (mM) either 300 potassium phosphate (pH 7), 10 thymidine, 1 ATP, 0.1 GTP, 10 MgC12, 1 EDTA and 5-100 jig of pre-activated cholera toxin (Ribeiro-Neto et al, 1985), or 25 Tris-HCl (pH 8), 10 thymidine, 1 ATP, 0.1 GTP, 1 EDTA and 1,25-5 tLg of pre-activated pertussis toxin (Ribeiro-Neto et al, 1985). Reactions were started by addition of NAD+ to a final concentration of 0.1 mM.…”

Section: Pretreatment Ofpig Bbm With Toxinsmentioning

confidence: 99%