44. Characterization of a transplantable androgen-dependent human prostatic carcinoma (PC 82)

Romijn, J. A.; Steenbrugge, G.J. van; Oishi, Kenji; Bolt-deVries, Joan; Hohn, W.; Schröder, Fritz H.

doi:10.1016/0022-4731(79)90408-4

Cited by 4 publications

(4 citation statements)

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“…The human prostatic carcinoma PC-82 has been maintained in nude mice for almost ten years now and passed through 35 transplant generations. The hormone-dependence of the PC-82 tumor has been documented before [37], in particular with regard to the applications of steroid-containing Silastic implants to improve the androgen status of the host animal [38]. A standard T-implant (length 1.0 cm) was introduced to overcome the great variability of circulating T-levels in the plasma of male nude mice (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…The PC-82 tumor, a moderately differentiated adenocarcinoma of the prostate, initiated in the year 1977, has passed through 35 mouse generations so far and still possesses its original properties. Its origin and further characterization have been described extensively [28,37]. PC-82 tumor tissue was grown in nude mice of the Balb/c background obtained from our breeding facilities.…”

Section: Materials and Methods Tumor Materialsmentioning

confidence: 99%

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Transplantable human prostatic carcinoma (PC‐82) in athymic nude mice: I. Hormone dependence and the concentration of androgens in plasma and tumor tissue

Steenbrugge¹,

Dongen²,

Reuvers

et al. 1987

The Prostate

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This paper is the first part of a series of three describing a number of observations made on the PC-82 human prostatic carcinoma, xenografted into nude mice. The previously described androgen-dependence, one of the main properties of this tumor, has been the subject of subsequent studies. The impact of hormonal manipulation on the growth of the tumor and on plasma and tissue concentrations of androgens is discussed in this first part of the series. The great variability of plasma testosterone (T) levels in intact male mice (range: 1-90 nmol/liter) has been levelled out by the use of T-containing Silastic implants, resulting in levels ranging from 18 to 35 nmol/liter. Moreover, this route of administration also facilitated hormonal manipulation of tumor bearing mice. Androgen withdrawal from male mice with growing PC-82 tumors caused 80% tumor regression at ten weeks after androgen deprivation; the decline of the tumor volume followed a biphasic course. Delayed androgen substitution in castrated male mice grafted with PC-82 30 days before resulted in growth of the tumor tissue. This indicates that cells do not die and keep the capability to respond to androgens. It was concluded that the growth of the PC-82 tumor is not compatible with plasma T levels lower than 1 nmol/liter. Variable concentrations of endogenous T and dihydrotestosterone (DHT) were detected in total homogenates of PC-82 tumor tissue. Androgen withdrawal from T-implanted, tumor-bearing female mice caused a rapid reduction (90% within one day) of the tissue-T and a slower decline (up to 90% within seven to ten days) of tissue-DHT concentrations.

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Section: Discussionmentioning

confidence: 99%

Section: Materials and Methods Tumor Materialsmentioning

confidence: 99%

Transplantable human prostatic carcinoma (PC‐82) in athymic nude mice: I. Hormone dependence and the concentration of androgens in plasma and tumor tissue

Steenbrugge¹,

Dongen²,

Reuvers

et al. 1987

The Prostate

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“…Knowledge of prostatic tumors and their characteristics has increased by the isolation of several prostatic cancer cell lines derived from primary or secondary human tumor deposits [7-lo]. From these, the PC-82 cell line is one of the best characterized [8,11,12]. It can be propagated in nude mice and its growth is androgen dependent [ 13,141.…”

Section: Introductionmentioning

confidence: 99%

“…The solid tumor shows prostate carcinoma morphology, ie, a predominantly cribriform growth pattern. The stromal component and vascularization are of murine origin [8,11]. In this study we describe the generation and properties of monoclonal antibodies (McAbs) against this cell line.…”

Section: Introductionmentioning

confidence: 99%

Characterization of monoclonal antibodies raised against the prostatic cancer cell line PC‐82

et al. 1986

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For production of monoclonal antibodies (McAbs), hybrid cells were prepared by fusion of spleen cells of BALB/c mice immunized with the human prostatic cancer cell line PC-82 and the P3-X6(3)Ag8.653 murine myeloma cell line. Supernatants of approximately 500 hybrid clones were screened for prostate specific antibodies using an ELISA on PC-82 cells. A selection of antibodies was further tested for their specificity on a large series of different tissues. A broad cross reactivity pattern was obtained. Most cross reactivity was with pancreatic tissue, kidney, and bowel. One antibody turned out to react with prostate stromal cells. Two McAbs (ER-Pr 1 and ER-Pr 2) reacted solely with prostatic epithelium. Monoclonal antibody affinity chromatography combined with SDS-PAGE showed that both antibodies were directed against a 35-kD protein. Immunoblotting revealed that this protein is identical to prostatic antigen (PA). The epitope detected by ER-Pr 1 and ER-Pr 2 was largely preserved after formalin-fixation of prostatic tissues which renders these antibodies very suitable for routine examination of tissue sections.

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Human prostatic adenocarcinoma: Some characteristics of a serially transplantable line in nude mice (PC 82)

et al. 1980

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Transplantation of tissue from a cribriform human prostatic carcinoma resulted in a serially transplantable tumor line in nude mice, PC 82. During 2 1/2 years the tumor has not changed its histological appearance and remained a moderately differentiated adenocarcinoma. The cells contain large amounts of prostatic acid phosphatase. It has a slow rate of growth and regresses after castration and estrogen treatment. This preliminary information suggests that the PC 82 tumor line may be suitable model for hormone-dependent human prostatic cancer.

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44. Characterization of a transplantable androgen-dependent human prostatic carcinoma (PC 82)

Cited by 4 publications

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Transplantable human prostatic carcinoma (PC‐82) in athymic nude mice: I. Hormone dependence and the concentration of androgens in plasma and tumor tissue

Transplantable human prostatic carcinoma (PC‐82) in athymic nude mice: I. Hormone dependence and the concentration of androgens in plasma and tumor tissue

Characterization of monoclonal antibodies raised against the prostatic cancer cell line PC‐82

Human prostatic adenocarcinoma: Some characteristics of a serially transplantable line in nude mice (PC 82)

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