[43] Monitoring of air and water for enzyme inhibitors

Goodson, Louis H.; Jacobs, William B.

doi:10.1016/s0076-6879(76)44045-4

Cited by 39 publications

(13 citation statements)

References 2 publications

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“…According to some researchers use of acidic conditions is a better choice for electrochemical detection of cysteine [33]. This may be attributed to the availability of more sulfhydryl groups in acidic pH than in basic, as in acidic pH the oxidation of sulfhydryl to disulfide is kinetically slow [34]. …”

Section: Effect Of Phmentioning

confidence: 99%

Ormosil Encapsulated Pyrroloquinoline Quinone‐Modified Electrochemical Sensor for Thiols

et al. 2004

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An organically modified sol-gel glass (ORMOSIL) encapsulating pyrroloquinoline quinone (PQQ)-modified electrode for the rapid, sensitive and simple determination of thiol-containing compounds such as cysteine and glutathione is reported. The effect of applied potential, nature of thiol compound and pH on the response of the sensor was examined and optimum conditions were determined. The electrochemical responses and detection limits were found to be sensitive to the nature of thiols and pH. The electrochemical responses for cysteine and glutathione at an applied potential of À 0.2 V (vs. Ag/AgCl) were found to be linear with detection limits of 18 nM for cysteine and 36 nM for glutathione at pH 3.5, whereas the detection limits at pH 8.5 were 0.5 mM for cysteine and 1 mM for glutathione. The electrode retained 95% of the original response for 7 days when stored at 4 8C. The ORMOSILencapsulated PQQ was also characterized by spectrophotometry. The absorbance measurement using 5,5'-dithiobis-(2-nitrobenzoic acid) at 412 nm justify the PQQ-mediated oxidation of glutathione whereas fluorescence measurements (excitation wavelength ¼ 380 nm; emission wavelength ¼ 480 nm) justify the successful encapsulation of PQQ in ORMOSIL matrix.

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Section: Effect Of Phmentioning

confidence: 99%

Ormosil Encapsulated Pyrroloquinoline Quinone‐Modified Electrochemical Sensor for Thiols

et al. 2004

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“…Pesticides (organophosphorus compounds and carbamates) are known to be inhibitors of cholinesterase activity and this effect has been monitored through potentiometry [9,10], piezoelectric devices [11], fibre optic systems [12,13], and amperometry [14][15][16][17][18]. Previous work in this laboratory [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19] showed the use of platinum electrodes for amperometric measurements with choline oxidase and acetylcholinesterase.…”

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confidence: 99%

Ruthenized screen-printed choline oxidase-based biosensors for measurement of anticholinesterase activity

et al. 1995

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Abstract. Plastic disposable choline biosensors based on ruthenized-carbon screen-printed electrodes were prepared and their use for monitoring organophosphorus pesticides and carbamates is described. The presence of 0.5% ruthenium on activated carbon mixed to form a simple graphite-based ink for the working electrode surface increased the sensitivity towards hydrogen peroxide. The choline biosensor is based on such an electrode coupled with choline oxidase immobilized by adsorption and was used to detect the inhibition effect of carbamates and organophosphorus pesticides on acetylcholinesterase. With the optimized procedure described (pH, buffer composition, incubation time, substrate concentration), concentrations of pesticides (Carbofuran) as low as 1 nM could be detected.

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“…It can easily be replaced by a fresh one when the enzyme activity decreases below a desired level. Using the above components, Goodson and Jacobs (1976) constructed a continuous aqueous monitor (CAM-I) for an unattended operation. The system works on the basis of a computerized detection cycle.…”

Section: Pesticidesmentioning

confidence: 99%

Biosensors for Environmental Control

Neujahr

1984

Biotechnology and Genetic Engineering Reviews

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[43] Monitoring of air and water for enzyme inhibitors

Cited by 39 publications

References 2 publications

Ormosil Encapsulated Pyrroloquinoline Quinone‐Modified Electrochemical Sensor for Thiols

Ormosil Encapsulated Pyrroloquinoline Quinone‐Modified Electrochemical Sensor for Thiols

Ruthenized screen-printed choline oxidase-based biosensors for measurement of anticholinesterase activity

Biosensors for Environmental Control

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