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Reamon-Büttner, Stella Marie; Schondelmaier, J.; Jung, Christian

doi:10.1023/a:1009650221460

Cited by 63 publications

(2 citation statements)

References 17 publications

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“…Researchers have previously reported the identification of other types of sex specific DNA markers in asparagus such as the SCAR and RAPD markers (Jiang & Sink, 1997;Lewis & Sink, 1996;Restivo et al, 1995). Similarly, STS and AFLP markers were adopted to carry out the fine mapping of the L5 (sex-related chromosome) in asparagus (Reamon-Büttner et al, 1998;Reamon-Büttner & Jung, 2000). However, these genetic markers were found limited to a certain group of cultivars.…”

Section: Discussionmentioning

confidence: 99%

Establishment of male‐specific sequence‐tagged site markers in Asparagus officinalis: An efficient tool for sex identification

Ahmad

Tian

et al. 2022

Plant Breeding

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Garden asparagus is a major dioecious plant that serves as a model for understanding sex determination and sex chromosome evolution. The shared cytogenetic homology of the sex chromosomes, with the exception of a mega base region missing from the X chromosome, has considerably hindered the identification of male and female plants. In this study, two male-specific sequence-tagged site (STS) markers Asp-YS1 and Asp-YS2, located on the Y chromosome, were discovered for early sex identification. The Asp-YS1 and Asp-YS2 markers were particularly effective in amplifying a fragment of 490-bp and 296-bp, respectively. Most of the male progenies of selected cultivars, and one homozygous male cultivar ('Y14'), showed male-specific amplification using these two markers. Of the 24 tested cultivars, the results of 21 cultivars demonstrated male-specific amplification using the newly identified STS markers suggesting greater applicability and higher specificity over the previously existed Asp1-T7sp marker, which is limited to some commercial cultivars. These findings presented the establishment of an efficient molecular method for sex identification in asparagus, enabling the rapid selection of male parental lines in future breeding programmes.

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Section: Discussionmentioning

confidence: 99%

Establishment of male‐specific sequence‐tagged site markers in Asparagus officinalis: An efficient tool for sex identification

Ahmad

Tian

et al. 2022

Plant Breeding

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“…In general, genes with sexually dimorphic expression are often referred to as “sex‐biased” genes, including those genes with sex‐specific or sex‐enriched expression (Ellegren & Parsch, 2007; Martins et al., 2013). Sex‐biased gene expression has been well documented in plants, and the identification of specific sex‐linked markers is used in sex determination in many commercially important plants like asparagus (Reamon‐Büttner et al., 1998), papaya (Deputy et al., 2002), and kiwifruit (Shirkot et al., 2002). Sex‐biased genes often evolve rapidly, and their differential expression regulates the sexual dimorphism as males and females (Parsch & Ellegren, 2013).…”

Section: Introductionmentioning

confidence: 99%

Identification of reproductive sex‐biased gene expression in Asparagopsis taxiformis (lineage 6) gametophytes

Patwary,

Zhao,

Paul

et al. 2023

Journal of Phycology

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The sub‐tropical red seaweed Asparagopsis taxiformis is of significant interest due to its ability to store halogenated compounds, including bromoform, which can mitigate methane production in ruminants. Significant scale‐up of aquaculture production of this seaweed is required; however, relatively little is known about the molecular mechanisms that control fundamental physiological processes, including the regulatory factors that determine sexual dimorphism in gametophytes. In this study, we used comparative RNA‐sequencing analysis between different morphological parts of mature male and female A. taxiformis (lineage 6) gametophytes that resulted in greater number of sex‐biased gene expression in tips (containing the reproductive structures for both sexes), compared with the somatic main axis and rhizomes. Further comparative RNA‐seq against immature tips was used to identify 62 reproductive sex‐biased genes (59 male‐biased, 3 female‐biased). Of the reproductive male‐biased genes, 46% had an unknown function, while others were predicted to be regulatory factors and enzymes involved in signaling. We found that bromoform content obtained from female samples (8.5 ± 1.0 mg·g−1 dry weight) was ~10% higher on average than that of male samples (6.5 ± 1.0 mg·g−1 dry weight), although no significant difference was observed (p > 0.05). There was also no significant difference in the marine bromoform biosynthesis locus gene expression. In summary, our comparative RNA‐sequencing analysis provides a first insight into the potential molecular factors relevant to gametogenesis and sexual differentiation in A. taxiformis, with potential benefits for identification of sex‐specific markers.

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Asparagus

Anido

Cointry

2008

Vegetables II

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Untitled

Cited by 63 publications

References 17 publications

Establishment of male‐specific sequence‐tagged site markers in Asparagus officinalis: An efficient tool for sex identification

Establishment of male‐specific sequence‐tagged site markers in Asparagus officinalis: An efficient tool for sex identification

Identification of reproductive sex‐biased gene expression in Asparagopsis taxiformis (lineage 6) gametophytes

Asparagus

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