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Chapon, Alain; Boutin, Morgane; Rimé, Delphine; Delalande, Laurie; Guillerm, Anne-Yvonne; Sarniguet, Alain

doi:10.1023/a:1022084728087

Cited by 15 publications

(3 citation statements)

References 39 publications

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“…Instead, a sequence that is unique for OSU142 was sought and primers were designed to detect the bacteria, a technique called RAPD-SCAR (28). It can quantify the bacteria and does not require genetic engineering (19,29), thus advantageous over other techniques such as molecular probes and hybridization-based techniques. The release of genetically engineered organisms to the environment is strictly forbidden in many countries, including Turkey (30).…”

Section: Discussionmentioning

confidence: 99%

“…These primers can be used individually or as a complementary tool to cultivation-based techniques to monitor the bacteria in any medium. Several primers have been successfully developed for other beneficial bacteria, including Pseudomonas fluorescens, Pantoea agglomerans, Bacillus subtilis, Pseudomonas brassicacearum , Azospirillum brasilense in strain-specific levels (19,(33)(34)(35)(36). These primers can detect and quantify the bacteria of interest in complex matrices such as rhizosphere soil.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, once a polymorphic RAPD band is obtained, the RAPD primer that amplifies the band is usually converted into a set of SCAR (Sequence-Characterized Amplified Region) primers. These are typically elongated versions of the RAPD primers (19). Tracking bacteria with SCAR primers provides more specificity, simplicity, and reproducibility over RAPD primers.…”

Section: Introductionmentioning

confidence: 99%

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Development of strain-specific SCAR primers to beneficial rhizobacteria Bacillus subtilis OSU-142

Eroğlu

2022

Period Biol

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Background and purpose: Synthetic fertilizers damage the environment. Biofertilizers that consist of microorganisms emerge as an environmentally friendly alternative. Biofertilizers improve plant growth by mobilizing soil nutrients, triggering plant hormone synthesis, or competing with pathogenic bacteria. However, biofertilizers often fail due to insufficient colonization of the plant roots.Materials and methods: To explore the colonization dynamics of a bacterial strain commonly used in biofertilizers, Bacillus subtilis OSU-142 (OSU-142), developing a set of primers specific to OSU-142 was aimed. Since its genome is unknown, to identify genomic regions unique to OSU-142 strain, DNAs of more than 40 bacterial strains were fingerprinted, most of which belong to Bacillus subtilis using the Randomly Amplified Polymorphic DNA (RAPD) method.Results: This approach identified a polymorphic band at 880 bp, which was then cloned and sequenced. The sequence showed no perfect match to any known sequences in the tested genomic databases indicating that the region of OSU-142 DNA is highly unique. The primer was converted to Sequence-Characterized Amplified Region (SCAR) primers, and its functionality in detecting OSU-142 genome was confirmed. Newly designed primer set can specifically detect OSU-142.Conclusions: These primers can be useful for basic science or commercial applications on tracking OSU-142 in various environments, thus contributing to biofertilizers’ adoption in the long run.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Development of strain-specific SCAR primers to beneficial rhizobacteria Bacillus subtilis OSU-142

Eroğlu

2022

Period Biol

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The Biocontrol BacteriumPseudomonas FluorescensPf29Arp Strain Affects the Pathogenesis‐Related Gene Expression of the Take‐All FungusGaeumannomyces GraminisVar.Triticion Wheat Roots

Daval

Lebreton

Sarniguet

2013

Molecular Microbial Ecology of the Rhizosphere

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SCAR marker: A potential tool for authentication of agriculturally important microorganisms

Ambreetha

Balachandar

2022

J Basic Microbiol

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Microbial inoculants are globally recommended for plant growth promotion and control of plant pathogens. These inoculants require stringent quality checks for sustainable field efficacy. Questionable regulatory frameworks constantly deteriorate the reliability of bio‐inoculant technology. Existing global regulations do not involve any rapid molecular technique for the routine inspection of microbial preparations. Sequence characterized amplified region (SCAR) marker offers rapid and precise strain‐level authentication of target microbes. Such advanced molecular techniques must be exploited to accurately validate the microbial formulations. Besides, the global dissemination of plant pathogenic microbes has always been an alarming threat to food security. SCAR markers could be used at the plant quarantine centers to rapidly detect catastrophic pathogens, thereby circumventing the import and export of contagious plant materials. The current review is focused on promoting the SCAR marker technology to validate commercial bio‐inoculants and predict plant pandemics.

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Untitled

Cited by 15 publications

References 39 publications

Development of strain-specific SCAR primers to beneficial rhizobacteria Bacillus subtilis OSU-142

Development of strain-specific SCAR primers to beneficial rhizobacteria Bacillus subtilis OSU-142

The Biocontrol BacteriumPseudomonas FluorescensPf29Arp Strain Affects the Pathogenesis‐Related Gene Expression of the Take‐All FungusGaeumannomyces GraminisVar.Triticion Wheat Roots

SCAR marker: A potential tool for authentication of agriculturally important microorganisms

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