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Song, Kyung Hwa; Lee, Jung-Kul; Song, Ji‐Soo; Hong, Soon Gyu; Baek, Hong; Kim, Sang Yong; Hyun, Hyung Hwan

doi:10.1023/a:1013824309263

Cited by 44 publications

(5 citation statements)

References 11 publications

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“…In media with glucose, 7.2 g l −1 mannitol and 23.4 g l −1 erythritol were found, whereas 39 and 20 g l −1 mannitol and a lack of erythritol production were observed upon application of fructose and glycerol, respectively. Song et al [44] isolated a strain of C. magnoliae that produced 67.0 g l −1 mannitol in fructose-containing medium.…”

Section: Resultsmentioning

confidence: 99%

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Production of erythritol and mannitol by Yarrowia lipolytica yeast in media containing glycerol

Tomaszewska

Rywińska

Gładkowski

2012

Journal of Industrial Microbiology and Biotechnology

167

114

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Glycerol is a by-product generated in large amounts during the production of biofuels. This study presents an alternative means of crude glycerol valorization through the production of erythritol and mannitol. In a shake-flasks experiment in a buffered medium, nine Yarrowia lipolytica strains were examined for polyols production. Three strains (A UV’1, A-15 and Wratislavia K1) were selected as promising producers of erythritol or/and mannitol and used in bioreactor batch cultures and fed-batch mode. Pure and biodiesel-derived crude glycerol media both supplemented (to 2.5 and 3.25 %) and not-supplemented with NaCl were applied. The best results for erythritol biosynthesis were achieved in medium with crude glycerol supplemented with 2.5 % NaCl. Wratislavia K1 strain produced up to 80.0 g l −1 erythritol with 0.49 g g −1 yield and productivity of 1.0 g l −1 h −1 . Erythritol biosynthesis by A UV’1 and A-15 strains was accompanied by the simultaneous production of mannitol (up to 27.6 g l −1 ). Extracellular as well as intracellular erythritol and mannitol ratios depended on the glycerol used and the presence of NaCl in the medium. The results from this study indicate that NaCl addition to the medium improves erythritol biosynthesis, and simultaneously inhibits mannitol formation.

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Section: Resultsmentioning

confidence: 99%

“…In the case of Wratislavia K1, the mannitol concentration was obtained 12 and 23 g l −1 in the glycerol and glucose media, respectively [37]. In the fed-batch cultures with optimized fructose media, different strains of C. magnoliae produced about 211 g l −1 mannitol, which corresponded to ~0.84 g g −1 yield [22, 44]. …”

Section: Resultsmentioning

confidence: 99%

Production of erythritol and mannitol by Yarrowia lipolytica yeast in media containing glycerol

Tomaszewska

Rywińska

Gładkowski

2012

Journal of Industrial Microbiology and Biotechnology

167

114

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“…However, it should be noted that mannitol was not the main product of these processes, but rather a side product during the biosynthesis of erythritol or citric acid from glycerol. The possibility of mannitol production was also studied using other yeast species and different substrates, e.g., n-paraffin by Candida zeylanoides , glucose by Torulopsis versatilis , and fructose by Candida magnoliae , which resulted in mannitol production of 52–67 g/L [ 22 , 23 ]. According to the literature, the highest concentration of mannitol (223 g/L) was obtained with the use of C. magnoliae HH-04 in a batch culture conducted in fructose medium supplemented with Ca 2+ and Cu 2+ ions [ 45 ].…”

Section: Resultsmentioning

confidence: 99%

“…Lactobacilli are auxotrophs that are not very resistant to changing environmental conditions and are small, while fungi form hyphae and are characterized by a long generation time. Studies also indicate the possibility of producing mannitol by the use of yeast [ 12 , 14 , 15 , 22 , 23 ]. These microorganisms can produce mannitol using glucose and fructose, but also less traditional substrates such as five-carbon sugars (e.g., Candida , Brettanomyces , Decceromyces , Kluyveromyces , Saccharomyces , Schwanniomyces , and Trichosporon ), glycerol ( Torulopsis versatilis and Candida azyma ), mannose, galactose, maltose, or xylitol [ 22 , 24 ].…”

Section: Introductionmentioning

confidence: 99%

Sugar Alcohol Sweetener Production by Yarrowia lipolytica Grown in Media Containing Glycerol

Juszczyk,

Rywińska,

Kosicka

et al. 2023

Molecules

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Most of the world’s annual production of mannitol is by chemical means, but, due to increasing demand for natural sweeteners, alternative production methods are being sought. The aim of the study was to screen Yarrowia lipolytica yeast strains and select culture conditions for the efficient and selective biosynthesis of mannitol from glycerol. From 21 strains examined in the shake-flask culture for mannitol biosynthesis from glycerol (100 g/L), three strains were selected—S2, S3, and S4—and further evaluated in batch bioreactor cultures with technical and raw glycerol (150 g/L). The best production parameters were observed for strain S3, which additionally was found to be the most resistant to NaCl concentration. Next, strain S3 was examined in batch culture with regard to the initial glycerol concentration (from 50 to 250 g/L). It was found that the substrate concentrations of 50 and 75 g/L resulted in the highest mannitol selectivity, about 70%. The fed-batch culture system proposed in this paper (performed in two variants in which glycerol was dosed in four portions of about 50 or 75 g/L) resulted in increased mannitol production, up to 78.5 g/L.

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“…Erythritol secretion in this condition was not observed. Until now, the best producer of mannitol described in the literature has been C. magnoliae growing in a fed-batch culture, reaching a mannitol concentration in the medium of 209 g/L [59]. To date, there have been no efficient mannitol producers described among Y. lipolytica strains which can secrete mannitol with high purity.…”

Section: Bioreactor Studiesmentioning

confidence: 99%

Honey’s Yeast—New Source of Valuable Species for Industrial Applications

Ziuzia

Janiec

Wróbel‐Kwiatkowska

et al. 2023

IJMS

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Honey is a rich source of compounds with biological activity; moreover, it is a valuable source of various microorganisms. The aim of this study was to isolate and identify yeast from a sample of lime honey from Poland as well as to assess its ability to biosynthesize value-added chemicals such as kynurenic acid, erythritol, mannitol, and citric acid on common carbon sources. Fifteen yeast strains belonging to the species Yarrowia lipolytica, Candida magnolia, and Starmerella magnoliae were isolated. In shake-flask screening, the best value-added compound producers were chosen. In the last step, scaling up of the culture in the bioreactor was performed. A newly isolated strain of Y. lipolytica No. 12 produced 3.9 mg/L of kynurenic acid growing on fructose. Strain Y. lipolytica No. 9 synthesized 32.6 g/L of erythritol on technical glycerol with a low concentration of byproducts. Strain Y. lipolytica No. 5 produced 15.1 g/L of mannitol on technical glycerol, and strain No. 3 produced a very high amount of citric acid (76.6 g/L) on technical glycerol. In conclusion, to the best of our knowledge this is the first study to report the use of yeast isolates from honey to produce valuable chemicals. This study proves that natural products such as lime honey can be an excellent source of wild-type yeasts with valuable production properties.

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Untitled

Cited by 44 publications

References 11 publications

Production of erythritol and mannitol by Yarrowia lipolytica yeast in media containing glycerol

Production of erythritol and mannitol by Yarrowia lipolytica yeast in media containing glycerol

Sugar Alcohol Sweetener Production by Yarrowia lipolytica Grown in Media Containing Glycerol

Honey’s Yeast—New Source of Valuable Species for Industrial Applications

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