[42] Industrial production of l-aspartic acid using polyurethane-immobilized cells containing aspartase

Fusee, Murray C.

doi:10.1016/s0076-6879(87)36044-6

Cited by 10 publications

(2 citation statements)

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“…Undoubtedly, aspartase represents one of the earliest success stories of biocatalysis for industrial applications. Its high specificity and activity were immediately recognized and exploited in several industrial processes for the preparation of l -aspartic acid from fumaric acid and ammonium salts, comprehensively reviewed elsewhere. , The earliest applications employed wild-type E. coli cells immobilized on various supports, such as polyacrylamide, polyurethane, or carrageenan, with high productivity, good stability, and, most importantly, low cost. Subsequent improvements on this process involved the use of recombinant cells overproducing aspartase and the application of thermotolerant DALs with increased stability under the process conditions .…”

Section: Synthetic Applications Of Ammonia-lyases and Aminomutasesmentioning

confidence: 99%

Synthetic and Therapeutic Applications of Ammonia-lyases and Aminomutases

et al. 2017

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Ammonia-lyases and aminomutases are mechanistically and structurally diverse enzymes which catalyze the deamination and/or isomerization of amino acids in nature by cleaving or shifting a C-N bond. Of the many protein families in which these enzyme activities are found, only a subset have been employed in the synthesis of optically pure fine chemicals or in medical applications. This review covers the natural diversity of these enzymes, highlighting particular enzyme classes that are used within industrial and medical biotechnology. These highlights detail the discovery and mechanistic investigations of these commercially relevant enzymes, along with comparisons of their various applications as stand-alone catalysts, components of artificial biosynthetic pathways and biocatalytic or chemoenzymatic cascades, and therapeutic tools for the potential treatment of various pathologies.

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Section: Synthetic Applications Of Ammonia-lyases and Aminomutasesmentioning

confidence: 99%

Synthetic and Therapeutic Applications of Ammonia-lyases and Aminomutases

et al. 2017

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“…Aspartase has been one of the early success stories in these applications, with the high specificity and catalytic activity of aspartase being exploited for the commercial production of L-aspartic acid. Escherichia coli cells have been immobilized in a polyacrylamide gel lattice (Tosa et al, 1974), in polyurethane (Fusee, 1987), or with carrageenan (Nishida et al, 1979). The aspartase activity in these immobilized cells shows a significantly higher stability against detergents and thermal denaturation (Tosa et al, 1977).…”

Section: Commercial Applicationsmentioning

confidence: 99%