[42] Analytical procedures for the isolation, identification, estimation, and investigation of the chlorophylls

Strain, Harold H.; Cope, B. T.; Svec, Walter A.

doi:10.1016/s0076-6879(71)23118-9

Cited by 231 publications

(125 citation statements)

References 56 publications

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“…The content of bacteriochlorophyll was tentatively determined by assuming the molar extinction coefficient as 1 X IO5 [7,8]. Growth was measured turbidimetrically at 660 nm with an Akiyama D.S.…”

Section: Methodsmentioning

confidence: 99%

Bacteriochlorophyll formation by facultative methylotrophs, Protaminobacter ruber and Pseudomonas AM 1

Sato

1978

FEBS Letters

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Section: Methodsmentioning

confidence: 99%

Bacteriochlorophyll formation by facultative methylotrophs, Protaminobacter ruber and Pseudomonas AM 1

Sato

1978

FEBS Letters

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“…Chl was isolated from dark-incubated Arabidopsis and pea leaves, or from N. benthamiana according to Pružinská et al (2005) and chl concentrations were determined spectrophotometrically (Strain et al, 1971). Chl and green pigments (chlide and pheide) were extracted and analyzed as described (Pružinská et al, 2005).…”

Section: Analysis Of Chl and Chl Catabolitesmentioning

confidence: 99%

Stay-green protein, defective in Mendel’s green cotyledon mutant, acts independent and upstream of pheophorbide a oxygenase in the chlorophyll catabolic pathway

2008

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“…28 Basal electron transport was determined by illuminating chloroplasts (20 µg mL -1 of chlorophyll) during 1 min in 3.0 mL of the reacting medium: 100 mmol L -1 sorbitol, 5 mmol L -1 MgCl 2 , 10 mmol L -1 KCl, 0.5 mmol L -1 KCN, 30 mmol L -1 Na + -tricine, and 50 mmol L -1 MV at pH 8.0. The sample was illuminated in the presence or absence of 6 mmol L -1 NH 4 Cl.…”

Section: Measurement Of Non-cyclic Electron Transport Ratementioning

confidence: 99%

“…28 Measurement of non-cyclic electron transport rate Light-induced non-cyclic electron transport activity from water to MV was determined by using a Clark type electrode, in the presence of 50 mmol L -1 of MV as electron acceptor. 28 Basal electron transport was determined by illuminating chloroplasts (20 µg mL -1 of chlorophyll) during 1 min in 3.0 mL of the reacting medium: 100 mmol L -1 sorbitol, 5 mmol L -1 MgCl 2 , 10 mmol L -1 KCl, 0.5 mmol L -1 KCN, 30 mmol L -1 Na + -tricine, and 50 mmol L -1 MV at pH 8.0. The sample was illuminated in the presence or absence of 6 mmol L -1 NH 4 Cl.…”

mentioning

confidence: 99%

Evaluation of herbicidal potential of depsides from Cladosporium uredinicola, an endophytic fungus found in Guava fruit

Medeiros¹,

Sampaio²,

Silva³

et al. 2012

J. Braz. Chem. Soc.

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Dois produtos naturais produzidos por Cladosporium uredinicola, um fungo endofítico isolado da goiaba, tiveram seus efeitos sobre a fotossíntese avaliados. Os compostos inibiram o fluxo de elétrons (basal, fosforilante e desacoplado) de água a metilviologênio (MV). Esses compostos atuam como inibidores da reação de Hill em tilacóides de espinafre frescos. Esses policetídeos pertencem à classe dos depsídeos e inibiram o fluxo de elétrons das reações parciais do fotossistema II (PS II) de água a 2,5-dicloro-1,4-benzoquinona (DCBQ), de água a silicomolibdato de sódio (SiMo Na + ), e ainda inibiram parcialmente de 1,5-difenilcarbazida (DPC) a 2,6-dicloroindofenol (DCPIP). Esses resultados estabeleceram que os sítios de inibição dos depsídeos estão localizados tanto no lado doador quanto no aceptor do PS II, entre P 680 e Q A . Medidas de fluorescência da clorofila a corroboraram para elucidar esse mecanismo de ação. Nenhum dos compostos inibiu o fluxo de elétrons no fotossistema I (PS I).Two natural products produced by Cladosporium uredinicola, an endophytic fungus isolated from guava fruit, were evaluated for their effects on photosynthesis. Both of them inhibited electron flow (basal, phosphorylating, and uncoupled) from water to methylviologen (MV), acting as Hill reaction inhibitors in freshly lysed spinach thylakoids. These polyketides, belonging to depsides class, inhibited partial reactions of photosystem II (PS II) electron flow from water to 2,5-dichloro-1,4-benzoquinone (DCBQ), from water to sodium silicomolybdate (SiMo Na + ), and partially inhibited electron flow from 1,5-diphenylcarbazide (DPC) to 2,6-dichloroindophenol (DCPIP). These results established that the depsides sites of inhibition are located on the donor and acceptor sides of PS II, between P 680 and Q A . Chlorophyll a fluorescence measurements corroborated this mechanism of action. None of the tested compounds inhibited photosystem I (PS I) electron transport.Keywords: endophyte, Cladosporium, depsides, Hill reaction inhibitors, photosystem II IntroductionExtensive research has been carried out worldwide to find new effective and ecologically natural friendly alternatives due to ecological restrictions in applying synthetic herbicides. A possible solution is the use of weed pathogens for biological control, using fungal metabolites to produce new selective herbicides such as biolaphos. 1 Therefore, these compounds could act as communicators between plants and fungi. 2,3 The interests of chemical ecology are to describe the ecological interactions mediated by natural products and their potential application. 3 The interactions depend on the organisms involved and their environments.Natural products have an enormous diversity of chemical structures and could show modes of action different than the commercial herbicides, suggesting that they are ideal leads for new herbicide discovery. 2,4,5 Recently, herbicidal activity of many natural products isolated from fungi, like stagonalide, 6 stagonolide H, 2 alternethanoxin A and B, 7 lasiodiplodi...

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[42] Analytical procedures for the isolation, identification, estimation, and investigation of the chlorophylls

Cited by 231 publications

References 56 publications

Bacteriochlorophyll formation by facultative methylotrophs, Protaminobacter ruber and Pseudomonas AM 1

Bacteriochlorophyll formation by facultative methylotrophs, Protaminobacter ruber and Pseudomonas AM 1

Stay-green protein, defective in Mendel’s green cotyledon mutant, acts independent and upstream of pheophorbide a oxygenase in the chlorophyll catabolic pathway

Evaluation of herbicidal potential of depsides from Cladosporium uredinicola, an endophytic fungus found in Guava fruit

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