411 - Role of adsorption in the electrochemical behaviour of nicotinamide adenine dinucleotide at a gold electrode

Takamura, Kiyoko; Mori, Atsuko; Kusu, Fumiyo

doi:10.1016/0302-4598(81)80044-x

Cited by 25 publications

(13 citation statements)

References 17 publications

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“…Consequently, the probability for two radicals to dimerize also increases. Nevertheless, the amount of active NADH formed at low overpotentials was surprisingly high compared to the available literature data on the direct regeneration of NADH on other non-modified electrodes [2,6,12]. To the best of our knowledge, no literature reporting a potential dependence of the amount of active NADH formed on other electrodes is available, and thus it is not possible to compare the results in Fig.…”

Section: Enzymatic Assay-number Of Electrons Involved In Nad + Reductioncontrasting

confidence: 57%

“…−1.28 V versus SCE on a glassy carbon electrode (GC) in phosphate buffer at pH 7. Takamura et al [12] observed also one reduction peak on a gold electrode in phosphate buffer pH 8.3, but at lower overpotential, −0.945 V versus SCE. Omanovic and co-workers [25,26] have shown that the cathodic peak on a RuGC electrode corresponds to the two-electron reduction of NAD + to NADH (Scheme 1), while Moiroux and Elving [11] interpreted the peak recorded on a bare GC electrode as the reduction of NAD + to the NAD 2 dimer (one-electron process).…”

Section: Resultsmentioning

confidence: 94%

“…In addition, the polarographic wave of the second reduction step was observed only in the presence of a surface-active electrolyte. Thus, the direct reduction of NAD + on non-modified electrodes results in a low yield of enzymatically active 1,4-NADH [2,6,12].…”

Section: Introductionmentioning

confidence: 99%

“…that could be potentially used to influence both the kinetics and mechanisms of the NAD + reduction reaction in order to increase the yield of the active NADH regenerated [13,14]. To the best of our knowledge, only Takamura et al [12] have published a study on the reduction of NAD + on a polycrystalline gold electrode. However, the paper discusses the interaction of NAD + with the Au surface in terms of adsorption, rather than in terms of the reduction kinetics.…”

Section: Introductionmentioning

confidence: 99%

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Electrochemical reduction of NAD+ on a polycrystalline gold electrode

Damian

Omanovic

2006

Journal of Molecular Catalysis A: Chemical

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Section: Enzymatic Assay-number Of Electrons Involved In Nad + Reductioncontrasting

confidence: 57%

Section: Resultsmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Electrochemical reduction of NAD+ on a polycrystalline gold electrode

Damian

Omanovic

2006

Journal of Molecular Catalysis A: Chemical

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“…It has been established that the direct electrochemical reduction of NAD + proceeds through the following two simplified steps:

S t e p 1 : {N A D}^{+} + {normale}^{-} \to N A D •

S t e p 2 a : N A D • + {normale}^{-} + {normalH}^{+} \to N A D H

where Step 2a – Equation represents a rate‐determining step, favouring the dimerization of the radical to produce enzymatically inactive dimer (NAD 2 ):

S t e p 2b : N A D • + N A D • \to {N A D}_{2}

…”

Section: Introductionmentioning

confidence: 99%

Direct electrochemical regeneration of enzymatic cofactor 1,4‐NADH on a cathode composed of multi‐walled carbon nanotubes decorated with nickel nanoparticles

et al. 2017

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Multi‐walled carbon nanotubes (MWCNTs) were grown on a stainless steel mesh and decorated with nickel nanoparticles (Ni NPs). The developed Ni NP‐MWCNT material was then used as a cathode in an electrochemical batch reactor to electrocatalytically convert NAD+ to enzymatically‐active 1,4‐NADH. The regeneration of 1,4‐NADH was studied at various electrode potentials. At electrode potential of −1.6 V, a very high recovery (relative amount of 1,4‐NADH in the product mixture) was obtained, 98 ± 1 %. In comparison, to achieve the same recovery on a non‐decorated MWCNT cathode, a much higher cathodic potential was needed (−2.3 V), establishing the importance of Ni NPs on the electrocatalytic activity in reducing NAD+ to 1,4‐NADH. It was postulated that hydrogen adsorbs on Ni NPs immobilized on MWCNTs to form Ni‐Hads, and this activated hydrogen rapidly reacts with neighbouring NAD‐radicals, preventing the dimerization of the latter species, ultimately yielding 1,4‐NADH.

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Electro‐Optical Reflection Methods for Studying Bioactive Substances at Electrode‐Solution Interfaces—an Approach to Biosurface Behavior

Takamura

Kusu

1987

Methods of Biochemical Analysis

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411 - Role of adsorption in the electrochemical behaviour of nicotinamide adenine dinucleotide at a gold electrode

Cited by 25 publications

References 17 publications

Electrochemical reduction of NAD+ on a polycrystalline gold electrode

Electrochemical reduction of NAD+ on a polycrystalline gold electrode

Direct electrochemical regeneration of enzymatic cofactor 1,4‐NADH on a cathode composed of multi‐walled carbon nanotubes decorated with nickel nanoparticles

Electro‐Optical Reflection Methods for Studying Bioactive Substances at Electrode‐Solution Interfaces—an Approach to Biosurface Behavior

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