405 What Is the Most Accurate Mri Approach to Assess Synovitis and/or Effusion in Knee Oa?

Loeuille, Damien; Saulière, Norbert; Champigneulle, J.; Blum, Andrew S.; Rat, A.-C.; Pourel, J; Chary‐Valckenaere, Isabelle

doi:10.1016/s1063-4584(08)60446-x

Cited by 7 publications

(6 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Synovial fibroblasts synthesize hyaluronic acid (HA), a glycosaminoglycan, and proteoglycan-4 (PRG4), a mucinous glycoprotein, which are major components of synovial fluid (SF) with important roles in joint lubrication [29,30]. In synovitis, synovial membranes become hyperplastic with increased accumulation of macrophages and other inflammatory cells, along with subintimal fibrosis and angiogenesis [13,14,31]. Synovial fibrosis can be considered a maladaptive healing response to chronic synovitis which is characterized by the upregulation of collagen type-I expression, fibroblast transition to a myofibroblast-like phenotype through de-novo expression of alpha smooth muscle actin (a-SMA) and enhanced migratory behavior, and upregulation of procollagen-lysine, 2-oxogluterate 5-dioxygenase 2 (PLOD2) leading to accumulation of cross-linked collagen in the synovium [32][33][34].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Proteoglycan-4 Regulates Fibroblast to Myofibroblast Transition and Expression of Fibrotic Genes in the Synovium

Qadri

Jay

Zhang

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Synovial tissue fibrosis is common in advanced OA with features including the presence of stress fiber-positive myofibroblasts and deposition of cross-linked collagen type-I. Proteoglycan-4 (PRG4) is a mucinous glycoprotein secreted by synovial fibroblasts and is a major component of synovial fluid. PRG4 is a ligand of the CD44 receptor. Our objective was to examine the role of PRG4-CD44 interaction in regulating synovial tissue fibrosis in vitro and in vivo. Methods: OA synoviocytes were treated with TGF-β ± PRG4 for 24 hours and α-SMA content was determined using immunofluorescence. Rhodamine labeled rhPRG4 was incubated with OA synoviocytes ± anti-CD44 or isotype control antibodies and cellular uptake of rhPRG4 was determined following a 30-min incubation and a-SMA expression following a 24-hour incubation. HEK-TGF-β cells were treated with TGF-β ± rhPRG4 and Smad3 phosphorylation was determined using immunofluorescence and TGF-β/Smad pathway activation was determined colorimetrically. We probed for stress fibers and focal adhesions (FAs) in TGF-β treated murine fibroblasts and fibroblast migration was quantified ± rhPRG4. Synovial expression of fibrotic markers: α-SMA, collagen type-I and PLOD2 in Prg4 gene trap (Prg4GT) and recombined Prg4GTR animals was studied at 2 and 9 months of age. Synovial expression of α-SMA and PLOD2 was determined in 2-months old Prg4GT/GT&Cd44-/- and Prg4GTR/GTR&Cd44-/- animals. Results: PRG4 reduced α-SMA content in OA synoviocytes (p<0.001). rhPRG4 was internalized by OA synoviocytes via CD44 and CD44 neutralization attenuated rhPRG4’s antifibrotic effect (p<0.05). rhPRG4 reduced pSmad3 signal in HEK-TGF-β cells (p<0.001) and TGF-β/Smad pathway activation (p<0.001). rhPRG4 reduced the number of stress fiber-positive myofibroblasts, FAs mean size, and cell migration in TGF-β treated NIH3T3 fibroblasts (p<0.05). rhPRG4 inhibited fibroblast migration in a macrophage and fibroblast co-culture model without altering active or total TGF-β levels. Synovial tissues of 9-months old Prg4GT/GT animals had higher α-SMA, collagen type-I and PLOD2 (p<0.001) content and Prg4 re-expression reduced these markers (p<0.01). Prg4 re-expression also reduced α-SMA and PLOD2 staining in CD44-deficient mice. Conclusion: PRG4 is an endogenous antifibrotic modulator in the joint and its effect on myofibroblast formation is partially mediated by CD44, but CD44 is not required to demonstrate an antifibrotic effect in vivo.

show abstract

Section: Introductionmentioning

confidence: 99%

“…These pathological changes are induced in the OA synovium by transforming growth factor beta (TGF-b1) and its associated signaling pathways [35,36]. Synovial fibrosis also contributes to joint pain and stiffness observed in advanced OA [31,37].…”

Section: Introductionmentioning

confidence: 99%

Proteoglycan-4 Regulates Fibroblast to Myofibroblast Transition and Expression of Fibrotic Genes in the Synovium

Qadri

Jay

Zhang

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…Another study in knee OA patients has shown a very good correlation between the degree of synovitis (thickness) seen by CE MRI and the degree of synovial inflammation measured by histology 10 . The same group has recently shown 11 that a good correlation also exists between the WORMS synovitis score (non-CE imaging) and the CE MRI score used in their previous study (r ¼ 0.54, P < 0.016). In that context, it is therefore not surprising that the results from our study 1 and the above study 10 are quite similar.…”

mentioning

confidence: 59%

“…The statistical analysis method used, the non-parametric Spearman correlation, is one simple way to assess synovial tissue inflammation reliability among and between readers over time on a semi-quantitative score. These simple correlations of intra and inter-reader reliability of MRI synovitis reading have previously been used by other investigators 11,13 . The excellent correlations demonstrated by our study compared to others may be related to the fact that we specifically measured the synovial thickness in mm using a software tool and then transposed the value into a semi-quantitative scale.…”

mentioning

confidence: 99%

Response to the Letter to the Editor by Roemer and collaborators entitled “Semiquantitative assessment of synovitis in osteoarthritis on non contrast-enhanced MRI” concerning the article published by Pelletier et al. entitled “A new non-invasive method to assess synovitis severity in relation to symptoms and cartilage volume loss in knee osteoarthritis patients using MRI”

Pelletier

Raynauld

Abram

et al. 2009

Osteoarthritis and Cartilage

View full text Add to dashboard Cite

show abstract

“…The production of mediators in synovial tissue leads to the activation of chondrocytes, which produce catabolic factors, and, at the same time, stimulate production of other cytokines, which promotes angiogenesis, perpetuating the cascade of cartilage destruction. Synovitis has been correlated with pain and associated with disease progression and severity ( 66 , 67 ). In the OA synovitis is not usually randomly distributed, but has a predilection for cartilage-pannus junction.…”

Section: Mrimentioning

confidence: 99%

Imaging of knee osteoarthritis: a review of multimodal diagnostic approach

Piccolo¹,

Mallio²,

Vaccarino³

et al. 2023

Quant Imaging Med Surg

View full text Add to dashboard Cite

Knee osteoarthritis (KOA) is a common chronic condition among the elderly population that significantly affects the quality of life. Imaging is crucial in the diagnosis, evaluation, and management of KOA. This manuscript reviews the various imaging modalities available until now, with a little focus on the recent developments with Artificial Intelligence. Currently, radiography is the first-line imaging modality recommended for the diagnosis of KOA, owing to its wide availability, affordability, and ability to provide a clear view of bony components of the knee. Although radiography is useful in assessing joint space narrowing (JSN), osteophytes and subchondral sclerosis, it has limited effectiveness in detecting early cartilage damage, soft tissue abnormalities and synovial inflammation. Ultrasound is a safe and affordable imaging technique that can provide information on cartilage thickness, synovial fluid, JSN and osteophytes, though its ability to evaluate deep structures such as subchondral bone is limited. Magnetic resonance imaging (MRI) represents the optimal imaging modality to assess soft tissue structures. New MRI techniques are able to detect early cartilage damage measuring the T1ρ and T2 relaxation time of knee cartilage. Delayed gadolinium-enhanced MRI of cartilage, by injecting a contrast agent to enhance the visibility of the cartilage on MRI scans, can provide information about its integrity. Despite these techniques can provide valuable information about the biochemical composition of knee cartilage and can help detect early signs of osteoarthritis (OA), they may not be widely available. Computed tomography (CT) has restricted utility in evaluating OA; nonetheless, weight-bearing CT imaging, using the joint space mapping technique, exhibits potential in quantifying knee joint space width and detecting structural joint ailments. PET-MRI is a hybrid imaging technique able to combine morphological information on bone and soft tissue alterations with the biochemical changes, but more research is needed to justify its high cost and time involved. The new tools of artificial intelligence, including machine learning models, can assist in detecting patterns and correlations in KOA that may be useful in the diagnosis, grading, predicting the need for arthroplasty, and improving surgical accuracy.

show abstract

405 What Is the Most Accurate Mri Approach to Assess Synovitis and/or Effusion in Knee Oa?

Cited by 7 publications

References 0 publications

Proteoglycan-4 Regulates Fibroblast to Myofibroblast Transition and Expression of Fibrotic Genes in the Synovium

Proteoglycan-4 Regulates Fibroblast to Myofibroblast Transition and Expression of Fibrotic Genes in the Synovium

Imaging of knee osteoarthritis: a review of multimodal diagnostic approach

Contact Info

Product

Resources

About