4′-phosphopantetheine acts as a potential antioxidant to limit atherosclerotic plaque formation by inhibiting ROS generation

Abstract

Coronary heart disease (CHD) is caused by coronary atherosclerosis and has a high morbidity and mortality rate worldwide. There are challenges in both early screening and treatment of CHD. The appearance and development of CHD is a complex metabolic disorder process. Therefore, to search for new biomarkers of CHD, we analyzed the peripheral blood metabolome in patients with CHD. In the study, a plasma metabolite, 4′-Phosphopantetheine (4-PPanSH), which was discovered by HPLC-MS/MS, as peripheral blood 4-PPanSH decreases, the degree of coronary blockage gradually aggravates. In addition, the 4-PPanSH supplement limited atherosclerotic plaque formation and endothelial injury in mice. Further, in vascular endothelial cells, 4-PPanSH effectively inhibited ROS generation and ox-LDL accumulation. In summary, 4-PPanSH was associated with the degree of coronary stenosis, and the 4-PPanSH supplement reduced atherosclerotic plaque generation, which could be associated with 4-PPanSH acting as a potent antioxidant that inhibits ROS generation and alleviates vascular endothelial injury.

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“…However, the uptake of this intermediate in CoA biosynthesis primarily depends on the degradation of extracellular CoA by heat-unstable enzymes present in serum [121,122]. In addition to the scarce presence of endogenous CoA and 4'-phosphopantetheine in serum [121,123,124], our experiments, conducted exclusively with heat-inactivated FBS, support the presence of PA, rather than a PA derivative, in the C. neoformans-containing phagolysosome. Nonetheless, it remains to be precisely determined whether C. neoformans employs PA and/or its derivatives during intracellular residence, considering possible implications in the development of antifungal drugs.…”

“…However, the uptake of this intermediate in CoA biosynthesis primarily depends on the degradation of extracellular CoA by heat-unstable enzymes present in serum [ 121 , 122 ]. In addition to the scarce presence of endogenous CoA and 4’-phosphopantetheine in serum [ 121 , 123 , 124 ], our experiments, conducted exclusively with heat-inactivated FBS, support the presence of PA, rather than a PA derivative, in the C . neoformans -containing phagolysosome.…”