4‐Methylumbelliferone promotes the migration and odontogenetic differentiation of human dental pulp stem cells exposed to lipopolysaccharide in vitro

Chen, Weiting; Guan, Yun; Xu, Fangfang; Jiang, Beizhan

doi:10.1002/cbin.11579

Cited by 7 publications

(13 citation statements)

References 29 publications

(35 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They also proved that activation of p38 mitogen-activated protein kinase (MAPK) and extracellular signal-regulated kinase (ERK), but not NF-κB, signaling pathways are involved in LPS-mediated differentiation of hDPSCs [ 61 ]. LPS alone promotes the migration of DPSCs, however 4-Methylumbelliferone (4-mu) can further accelerate the migration and odontogenetic differentiation by downregulating the expression of inflammatory cytokines [ 62 ].…”

Section: Resultsmentioning

confidence: 99%

“…Some agents act antagonistically to E. coli LPS in hDPSCs. 4-Methylumbelliferone (4-mu) downregulates the expression of inflammatory cytokines (decreased CD44 expression, preventing LMW HA-TLR4-CD44 complex formation) and facilitates cell differentiation [ 62 ]. Epigallocatechin gallate (EGCG) has an anti-inflammatory effect, not affecting cell proliferation or differentiation [ 67 ].…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Role of Lipopolysaccharide, Derived from Various Bacterial Species, in Pulpitis—A Systematic Review

et al. 2022

View full text Add to dashboard Cite

Lipopolysaccharide (LPS) is widely used for induction of inflammation in various human tissues, including dental pulp. The purpose of this study was to summarize current medical literature focusing on (1) cell types used by researchers to simulate dental pulp inflammation, (2) LPS variants utilized in experimental settings and how these choices affect the findings. Our study was conducted in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA). We searched for studies reporting outcomes of lipopolysaccharide application on dental pulp cells in vitro using electronic databases: MEDLINE, Web of Science and Scopus. Having gathered data from 115 papers, we aimed to present all known effects LPS has on different cell types present in dental pulp. We focused on specific receptors and particles that are involved in molecular pathways. Our review provides an essential foundation for further research using in vitro models of pulpitis.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Role of Lipopolysaccharide, Derived from Various Bacterial Species, in Pulpitis—A Systematic Review

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Tooth decay, dental trauma, tooth erosion, and other dental problems can all stimulate inflammation in the dental pulp tissue, leading to pulp necrosis and even periapical periodontitis [ 1 ]. One of the prevalent dental conditions known as pulpitis is an opportunistic infection caused by oral bacteria, which cause tooth pulp inflammation [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of HM-Exos on the migration and inflammatory response of LPS-exposed dental pulp stem cells

et al. 2023

View full text Add to dashboard Cite

Aim The purpose of this study was to investigate the effects of human milk exosomes (HM-Exos) on the viability, migration, and inflammatory responses of lipopolysaccharide (LPS)-exposed human dental pulp stem cells (HDPSCs) in vitro. Methods HM-Exos were isolated, and dynamic light scattering (DLS), scanning electron microscopy (SEM), and transmission electron microscopy (TEM) were used to analyze their physical properties (size and shape). To construct an in vitro inflammation model, HDPSCs were exposed to LPS. The MTT test and migration assay were used to investigate the effect of HM-Exos on cell proliferation and migration, and the quantitative polymerase chain reaction (qPCR) was used to assess the expression of inflammatory genes in HDPSCs. Data were analyzed using a one-way analysis of variance (ANOVA) with Tukey's post-test. Results DLS measurement revealed that HM-Exos were 116.8 ± 3.6 nm in diameter. The SEM and TEM images revealed spherical shapes with diameters of 97.2 ± 34.6 nm. According to the results of the cell viability assay, the nontoxic concentration of HM-Exos (200 µg/ml) was chosen for the subsequent investigations. The migration assay results showed that HM-Exos improved the potential of LPS-exposed HDPSCs to migrate. The qPCR results indicated that HM-Exos significantly reduced the expression of inflammatory cytokines such as TNF-α, IL-1β, and IL-6 in HDPSCs after LPS stimulation. Conclusions HM-Exos increased LPS-exposed HDPSCs migration and proliferation and reduced gene expression of inflammatory cytokines. They may be a viable candidate for pulpitis therapy.

show abstract

“…The immunomodulatory potential of DPSCs may be of particular importance for pulp tissue to repair or regenerate under conditions of pulpitis. To date, many studies have focused on the role of DPSCs in the progression and treatment of pulpitis via establishing in vitro pulpitis models to simulate an inflammatory environment of DPSCs [ 7 , 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

“…Different stimuli such as LPS, TNF, bacterial extracts are used to imitate an inflammatory dental pulp microenvironment [ 27 – 29 ]. To stimulate DPSCs in establishing in vitro pulpitis models, many researchers use E. coli LPS [ 10 , 30 ] whereas others use P. gingivalis LPS [ 27 , 31 ]. LPS from E. coli , targets TLR4 and activates the downstream NF-κB signaling pathway, leading to the expression of inflammatory cytokines [ 32 ].…”

Section: Introductionmentioning

confidence: 99%

Different expression patterns of inflammatory cytokines induced by lipopolysaccharides from Escherichia coli or Porphyromonas gingivalis in human dental pulp stem cells

et al. 2022

View full text Add to dashboard Cite

Background Lipopolysaccharide (LPS) is one of the leading causes of pulpitis. The differences in establishing an in vitro pulpitis model by using different lipopolysaccharides (LPSs) are unknown. This study aimed to determine the discrepancy in the ability to induce the expression of inflammatory cytokines and the underlying mechanism between Escherichia coli (E. coli) and Porphyromonas gingivalis (P. gingivalis) LPSs in human dental pulp stem cells (hDPSCs). Material and methods Quantitative real-time polymerase chain reaction (QRT-PCR) was used to evaluate the mRNA levels of inflammatory cytokines including IL-6, IL-8, COX-2, IL-1β, and TNF-α expressed by hDPSCs at each time point. ELISA was used to assess the interleukin-6 (IL-6) protein level. The role of toll-like receptors (TLR)2 and TLR4 in the inflammatory response in hDPSCs initiated by LPSs was assessed by QRT-PCR and flow cytometry. Results The E. coli LPS significantly enhanced the mRNA expression of inflammatory cytokines and the production of the IL-6 protein (p < 0.05) in hDPSCs. The peaks of all observed inflammation mediators’ expression in hDPSCs were reached 3–12 h after stimulation by 1 μg/mL E. coli LPS. E. coli LPS enhanced the TLR4 expression (p < 0.05) but not TLR2 in hDPSCs, whereas P. gingivalis LPS did not affect TLR2 or TLR4 expression in hDPSCs. The TLR4 inhibitor pretreatment significantly inhibited the gene expression of inflammatory cytokines upregulated by E. coli LPS (p < 0.05). Conclusion Under the condition of this study, E. coli LPS but not P. gingivalis LPS is effective in promoting the expression of inflammatory cytokines by hDPSCs. E. coli LPS increases the TLR4 expression in hDPSCs. P. gingivalis LPS has no effect on TLR2 or TLR4 expression in hDPSCs.

show abstract

4‐Methylumbelliferone promotes the migration and odontogenetic differentiation of human dental pulp stem cells exposed to lipopolysaccharide in vitro

Cited by 7 publications

References 29 publications

Role of Lipopolysaccharide, Derived from Various Bacterial Species, in Pulpitis—A Systematic Review

Role of Lipopolysaccharide, Derived from Various Bacterial Species, in Pulpitis—A Systematic Review

Effect of HM-Exos on the migration and inflammatory response of LPS-exposed dental pulp stem cells

Different expression patterns of inflammatory cytokines induced by lipopolysaccharides from Escherichia coli or Porphyromonas gingivalis in human dental pulp stem cells

Contact Info

Product

Resources

About